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Human INS(Insulin) ELISA Kit

  • Cat.No.:E-EL-H2665

  • Reactivity: Human

To Purchase E-EL-H2665

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.78-50 μIU/mL
Sensitivity 0.47 μIU/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human INS in samples. No significant cross-reactivity or interference between Human INS and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human INS concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human INS. You can calculate the concentration of Human INS in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human INS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human INS were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (μIU/mL) 2.38 5.03 23.63 2.21 6.19 21.47
Standard deviation 0.13 0.25 1.25 0.11 0.29 0.97
CV (%) 5.46 4.97 5.29 4.98 4.68 4.52

Recovery

The recovery of Human INS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-99 94
EDTA plasma (n=8) 96-108 101
Cell culture media (n=8) 94-106 102

Linearity

Samples were spiked with high concentrations of Human INS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsIDDM2, ILPR, IRDN, MODY10
Research AreaCancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Science Advances (2022) IF: 14.957
    Glucose-responsive microneedle patch for closed-loop dual-hormone delivery in mice and pigs

    DOI: 10.1126/sciadv.add3197

    Sample: plasma
  2. MOLECULAR NUTRITION & FOOD RESEARCH (2022) IF: 6.575
    Branched Chain Amino Acids, New Target of Germinated Brown Rice against Type 2 Diabetes Mellitus: A Randomized Controlled Trial

    DOI: 10.1002/mnfr.202200481

    Sample: serum
  3. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2021) IF: 4.219
    Prevalence of ApoB100 rs693 gene polymorphism in metabolic syndrome among female students at King Abdulaziz University

    DOI: 10.1016/j.sjbs.2021.02.064

    PMID: 34121862

    Sample: Serum
  4. Nutrients (2020) IF: 4.546
    Vitamin E Levels in Ethnic Communities in Malaysia and Its Relation to Glucose Tolerance, Insulin Resistance and Advanced Glycation End Products: A Cross-Sectional Study

    DOI: 10.3390/nu12123659

    PMID: 33261162

    Sample: serum
  5. Nutrients (2020) IF: 4.546
    Intermittent Fasting Enhanced the Cognitive Function in Older Adults with Mild Cognitive Impairment by Inducing Biochemical and Metabolic changes: A 3-Year Progressive Study

    DOI: 10.3390/nu12092644

    PMID: 32872655

    Sample: Plasma
  6. Clinical Epidemiology (2021) IF: 4.79
    Cardiovascular Disease Prediction by Machine Learning Algorithms Based on Cytokines in Kazakhs of China

    DOI: 10.2147/CLEP.S313343

    PMID: 34135637

    Sample: blood
  7. AMINO ACIDS (2020) IF: 3.063
    Metabolomics and correlation network analyses of core biomarkers in type 2 diabetes

    DOI: 10.1007/s00726-020-02891-8

    PMID: 32930872

    Sample: Plasma
  8. DIABETES RESEARCH AND CLINICAL PRACTICE (2019) IF: 3.239
    Ramadan diurnal intermittent fasting modulates SOD2, TFAM, Nrf2, and sirtuins (SIRT1, SIRT3) gene expressions in subjects with overweight and obesity

    DOI: 10.1016/j.diabres.2019.107801

    Sample: serum
  9. ANIMAL FEED SCIENCE AND TECHNOLOGY (2021) IF: 3.247
    The effect of hydrothermal processing on the performance of broiler chicks fed corn or sorghum-based diets

    DOI: 10.1016/j.anifeedsci.2021.114953

    Sample: Serum
  10. DRUG AND CHEMICAL TOXICOLOGY (2022) IF: 3.356
    The protective effect of N-acetyl cysteine against selenium toxicity and gamma irradiation in rats

    DOI: 10.1080/01480545.2022.2058010

    PMID: 35361025

    Sample: blood
  11. MEDIATORS OF INFLAMMATION (2019) IF: 3.545
    Nomogram Based on Cytokines for Cardiovascular Diseases in Xinjiang Kazakhs

    DOI: 10.1155/2019/4756295

    Sample: plasma
  12. PLoS One (2022) IF: 3.752
    Comparing acute effects of extra virgin coconut oil and extra virgin olive oil consumption on appetite and food intake in normal-weight and obese male subjects

    DOI: 10.1371/journal.pone.0274663

    PMID: 36112590

    Sample: serum
  13. APPETITE (2021) IF: 3.868
    Acute and short-term effects of Lactobacillus paracasei subsp. paracasei 431 and inulin intake on appetite control and dietary intake: A two-phases randomized, double blind, placebo-controlled study

    DOI: 10.1016/j.appet.2021.105855

    PMID: 34915104

    Sample: serum
  14. Journal of Cosmetic Dermatology (2022) IF: 2.189
    Assessment of Serum Chemerin Levels in Acanthosis Nigricans: A Case Control Study

    DOI: 10.1111/jocd.15311

    Sample: serum
  15. Trials (2021) IF: 2.279
    Effect of Lycium barbarum polysaccharide supplementation in non-alcoholic fatty liver disease patients: study protocol for a randomized controlled trial

    DOI: 10.1186/s13063-021-05529-6

    PMID: 34521466

    Sample: plasma
  16. Obesity Research & Clinical Practice (2021) IF: 2.288
    Fermented soybean enhances post-meal response in appetite-regulating hormones among Indonesian girls with obesity

    DOI: 10.1016/j.orcp.2021.06.005

    PMID: 34147377

    Sample: plasma
  17. BIOSCIENCE REPORTS (2019) IF: 2.535
    Serum profile of cytokines and their genetic variants in metabolic syndrome and healthy subjects: a comparative study

    DOI: 10.1042/BSR20181202

    Sample: serum
  18. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2020) IF: 2.842
    Insulin Resistance is Associated with Gut Permeability Without the Direct Influence of Obesity in Young Adults

    DOI: 10.2147/DMSO.S256864

    PMID: 32922055

    Sample: Plasma
  19. Medicina-Lithuania (2022) IF: 2.948
    Ramadan Intermittent Fasting Is Associated with Changes in Circulating Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) in Metabolically Healthy Obese Subjects

    DOI: 10.3390/medicina58040503

    Sample: serum
  20. Journal of General and Family Medicine (2023)
    The role of biofield energy treatment on psychological symptoms, mental health disorders, and stress-related quality of life in adult subjects: A randomized controlled clinical trial

    DOI: 10.1002/jgf2.606

    Sample: serum
  21. Clinical Nutrition ESPEN (2022)
    Impacts of a nutrition education intervention in individuals with cardiometabolic risk: Protocol of a randomized controlled trial

    DOI: 10.1016/j.clnesp.2022.03.037

    Sample: plasma
  22. Diabetes & Metabolic Syndrome-Clinical Research & Reviews (2022)
    Effect of saccharin, a non-nutritive sweeteners, on insulin and blood glucose levels in healthy young men: A crossover trial

    DOI: 10.1016/j.dsx.2022.102500

    Sample: plasma
  23. Gene Reports (2021)
    Association of Apelin gene rs3115757 variation and expression with metabolic syndrome among female students in King Abdulaziz University

    DOI: 10.1016/j.genrep.2021.101305

    Sample: serum
  24. JOURNAL OF THE PAKISTAN MEDICAL ASSOCIATION (2019) IF: 0.642
    Peroxisome proliferator activated receptor gamma 34C>G variant and anthropometric parameters in metabolic syndrome

    PMID: 31511709

    Sample: serum
  25. Journal of PeriAnesthesia Nursing (2020) IF: 0.805
    Effect of Preoperative Oral Carbohydrate Administration on Patients Undergoing Cesarean Section with Epidural Anesthesia: A Pilot Study

    DOI: 10.1016/j.jopan.2020.05.006

    PMID: 33239219

    Sample: serum
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