Human KRT10 (Keratin, Type I Cytoskeletal 10) CLIA Kit (E-CL-H1268)

Name: Human KRT10 (Keratin, Type I Cytoskeletal 10) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1268
Price: 180.0 USD
Availability: InStock

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Human KRT10 (Keratin, Type I Cytoskeletal 10) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human KRT10 in samples.No significant cross-reactivity or interference between Human KRT10 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	48593.000	58655.000	53624.000	53595
250	20712.000	24816.000	22764.000	22735
125	10618.000	9200.000	9909.000	9880
62.5	4376.000	5068.000	4722.000	4693
31.25	2549.000	2275.000	2412.000	2383
15.63	1358.000	1166.000	1262.000	1233
7.81	775.000	807.000	791.000	762
0	28.000	30.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	27.480	65.370	171.250	27.440	67.270	165.600
Standard deviation	2.700	6.920	10.280	3.360	5.110	12.040
CV（%）	9.830	10.590	6.000	12.240	7.600	7.270

Recovery

The recovery of Human KRT10 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	97-108	102
EDTA plasma （n=8）	88-103	96
Cell culture media （n=8）	86-99	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human KRT10 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-102	94-110	100-113
1:2	Average	95	101	107
1:4	Range	96-111	86-99	101-113
1:4	Average	102	91	107
1:8	Range	96-113	84-96	88-102
1:8	Average	104	90	93

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human KRT10. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KRT10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KRT10, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human KRT10. You can calculate the concentration of Human KRT10 in the samples by comparing the RLU value of the samples to the standard curve.

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