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Human MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit

  • Cat.No.:E-EL-H6005

  • Reactivity: Human

To Purchase E-EL-H6005

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 62.50-4000 pg/mL
Sensitivity 37.50 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human MCP-1 in samples. No significant cross-reactivity or interference between Human MCP-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human MCP-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MCP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MCP-1. You can calculate the concentration of Human MCP-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MCP-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MCP-1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 198.91 488.33 1680.55 164.36 433.06 1730.78
Standard deviation 10.01 24.12 90.58 9.45 20.74 76.85
CV (%) 5.03 4.94 5.39 5.75 4.79 4.44


The recovery of Human MCP-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 97-108 101
EDTA plasma (n=8) 89-99 94
Cell culture media (n=8) 87-95 92


Samples were spiked with high concentrations of Human MCP-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P13500
SynonymsCCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF
Research AreaCancer, Cardiovascular, Metabolism, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


  1. Molecular Cancer (2021) IF: 27.401
    KDM6A-ARHGDIB axis blocks metastasis of bladder cancer by inhibiting Rac1

    DOI: 10.1186/s12943-021-01369-9

    PMID: 34006303

    Sample: Cell culture supernatant
  2. Theranostics (2021) IF: 11.556
    A SCID mouse-human lung xenograft model of SARS-CoV-2 infection

    DOI: 10.7150/thno.58321

    PMID: 33995679

    Sample: Tissue homogenate
  3. THROMBOSIS RESEARCH (2022) IF: 10.407
    High mobility group box 1 derived mainly from platelet microparticles exacerbates microvascular obstruction in no reflow

    DOI: 10.1016/j.thromres.2022.12.003

    Sample: Human cardiac microvascular endothelial cells (HCM
  4. Cell Death & Disease (2020) IF: 8.4689
    Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion

    DOI: 10.1038/s41419-020-2665-z

    PMID: 32532953

    Sample: Cell culture supernatant
    Tumor-Contacted Neutrophils Promote Metastasis by a CD90-TIMP-1 Juxtacrine–Paracrine Loop

    DOI: 10.1158/1078-0432.CCR-18-2544

    Sample: tumor-infiltrating neutrophils (TINs),neutrophils
  6. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Antidyslipidemic, Antioxidant, and Anti-inflammatory Effects of Jelly Drink Containing Polyphenol-Rich Roselle Calyces Extract and Passion Fruit Juice with Pulp in Adults with Dyslipidemia: A Randomized, Double-Blind, Placebo-Controlled Trial.

    DOI: 10.1155/2022/4631983

    Sample: plasma
    Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo

    DOI: 10.3390/ijms232416134

    Sample: THP-1 cells
  8. Cancers (2022) IF: 6.575
    The GBM Tumor Microenvironment as a Modulator of Therapy Response: ADAM8 Causes Tumor Infiltration of Tams through HB-EGF/EGFR-Mediated CCL2 Expression and Overcomes TMZ Chemosensitization in Glioblastoma

    DOI: 10.3390/cancers14194910

    PMID: 36230833

    Sample: U87MG cell,G1 cell
  9. Nutrients (2023) IF: 6.706
    Safety and Effects of Lactobacillus paracasei TISTR 2593 Supplementation on Improving Cholesterol Metabolism and Atherosclerosis-Related Parameters in Subjects with Hypercholesterolemia: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial

    DOI: 10.3390/nu15030661

    Sample: serum
  10. Drug Design Development and Therapy (2021) IF: 4.162
    Prunella vulgaris L. Attenuates Experimental Autoimmune Thyroiditis by Inhibiting HMGB1/TLR9 Signaling

    DOI: 10.2147/DDDT.S325814

    PMID: 34764638

    Sample: Nthy-ori 3–1 cells
    Gynura procumbens (Lour.) Merr. extract attenuates monocyte adherence to endothelial cells through suppression of the NF-κB signaling pathway

    DOI: 10.1016/j.jep.2022.115391

    PMID: 35589022

    Sample: HUVECs
  12. INFLAMMATION RESEARCH (2021) IF: 4.575
    Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis

    DOI: 10.1007/s00011-021-01459-w

    PMID: 33852061

    Sample: Serum,Bronchoalveolar Lavage Fluid
  13. ADDICTION BIOLOGY (2017) IF: 4.603
    Young alcohol binge drinkers have elevated blood endotoxin, peripheral inflammation and low cortisol levels: neuropsychological correlations in women

    DOI: 10.1111/adb.12543

    Sample: Plasma
  14. INFLAMMATION RESEARCH (2019) IF: 3.061
    Common variants of genes encoding TLR4 and TLR4 pathway members TIRAP and IRAK1 are effective on MCP1, IL6, IL1β, and TNFα levels in type 2 diabetes and insulin resistance

    DOI: 10.1007/s00011-019-01263-7

    Sample: serum
  15. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2021) IF: 3.168
    Inflammatory Biomarkers Levels in T2DM Emirati Patients with Diabetic Neuropathy

    DOI: 10.2147/DMSO.S319863

    PMID: 34345175

    Sample: serum
  16. American Journal of Translational Research (2019) IF: 3.266
    Profiling and functional characterization of circulation LncRNAs that are associated with coronary atherosclerotic plaque stability

    PMID: 31312390

    Sample: THP-1 cells
    Chronic fatigue and fibromyalgia symptoms are key components of deficit schizophrenia and are strongly associated with activated immune-inflammatory pathways

    DOI: 10.1016/j.schres.2020.05.003

    PMID: 32467068

    Sample: Serum
  18. BIOSCIENCE REPORTS (2019) IF: 3.84
    Up-regulated MCPIP1 in abdominal aortic aneurysm is associated with vascular smooth muscle cell apoptosis and MMPs production

    DOI: 10.1042/BSR20191252

    PMID: 31651935

    Sample: Serum,Plasma,Cell sample
  19. Journal of the International Society of Sports Nutrition (2019) IF: 3.841
    Effects of a polysaccharide-based multi-ingredient supplement on salivary immunity in non-elite marathon runners

    DOI: 10.1186/s12970-019-0281-z

    Sample: saliva
  20. Scientific Reports (2020) IF: 3.998
    Intermittent hypoxia modulates redox homeostasis, lipid metabolism associated inflammatory processes and redox post-translational modifications: Benefits at high altitude

    DOI: 10.1038/s41598-020-64848-x

    PMID: 32404929

    Sample: Plasma
  21. Trials (2021) IF: 2.279
    Effect of Lycium barbarum polysaccharide supplementation in non-alcoholic fatty liver disease patients: study protocol for a randomized controlled trial

    DOI: 10.1186/s13063-021-05529-6

    PMID: 34521466

    Sample: blood
  22. GENE (2019) IF: 2.638
    Downregulation of hsa_circ_0068087 ameliorates TLR4/NF-κB/NLRP3 inflammasome-mediated inflammation and endothelial cell dysfunction in high glucose conditioned by sponging miR-197

    DOI: 10.1016/j.gene.2019.05.012

    Sample: Human Umbilical Vein Endothelial Cells (HUVECs)
  23. PLoS One (2018) IF: 2.766
    Salivary immunity and lower respiratory tract infections in non-elite marathon runners

    DOI: 10.1371/journal.pone.0206059

    PMID: 30462646

    Sample: saliva
  24. PLoS One (2017) IF: 2.806
    Depot-specific inflammation with decreased expression of ATM2 in white adipose tissues induced by high-margarine/lard intake

    DOI: 10.1371/journal.pone.0188007

    Sample: Cell culture supernatant
    The Neuroimmune and Neurotoxic Fingerprint of Major Neurocognitive Psychosis or Deficit Schizophrenia: a Supervised Machine Learning Study

    DOI: 10.1007/s12640-019-00112-z

    Sample: serum
  26. International Journal of Clinical and Experimental Pathology (2018) IF: 1.396
    MicroRNA-124 alleviates hyperoxia-induced inflammatory response in pulmonary epithelial cell by inhibiting TLR4/NF-κB/CCL2

    PMID: 31938089

    Sample: Beas2B cells
  27. Experimental and Therapeutic Medicine (2020) IF: 1.785
    Curcumin affects ox‑LDL‑induced IL‑6, TNF‑α, MCP‑1 secretion and cholesterol efflux in THP‑1 cells by suppressing the TLR4/NF‑κB/miR33a signaling pathway

    DOI: 10.3892/etm.2020.8915

    Sample: Cell culture supernatant
  28. Therapeutic Advances in Ophthalmology (2020)
    Serum malondialdehyde, monocyte chemoattractant protein-1, and vitamin C levels in wet type age-related macular degeneration patients:

    DOI: 10.1177/2515841420951682

    Sample: Serum
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