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Human SEMA3A(Semaphorin 3A) ELISA Kit

  • Cat.No.:E-EL-H1274

  • Reactivity: Human

To Purchase E-EL-H1274

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human SEMA3A in samples. No significant cross-reactivity or interference between Human SEMA3A and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human SEMA3A concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SEMA3A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SEMA3A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SEMA3A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SEMA3A. You can calculate the concentration of Human SEMA3A in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human SEMA3A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human SEMA3A were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.55 0.89 4.77 0.60 0.88 5.24
Standard deviation 0.03 0.05 0.18 0.03 0.04 0.22
CV (%) 5.45 5.62 3.77 5.00 4.55 4.20

Recovery

The recovery of Human SEMA3A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-108 99
EDTA plasma (n=8) 87-98 92
Cell culture media (n=8) 89-104 96

Linearity

Samples were spiked with high concentrations of Human SEMA3A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q14563
Research AreaCancer, Cardiovascular, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Bone Research (2020) IF: 11.508
    Osteoblasts are inherently programmed to repel sensory innervation

    DOI: 10.1038/s41413-020-0096-1

    Sample: Cell culture medium
  2. Oncotarget (2016) IF: 5.008
    Axon guidance molecule semaphorin3A is a novel tumor suppressor in head and neck squamous cell carcinoma

    DOI: 10.18632/oncotarget.6831

    Sample: Cell culture supernatant
  3. JOURNAL OF PERIODONTAL RESEARCH (2022) IF: 4.419
    Gingival crevicular fluid lipocalin-2 and semaphorin3A in stage III periodontitis: Non-surgical periodontal treatment effects

    DOI: 10.1111/jre.12995

    PMID: 35468224

    Sample: gingival crevicular fluid (GCF)
  4. PLoS One (2021) IF: 3.24
    Extract of Scutellaria baicalensis induces semaphorin 3A production in human epidermal keratinocytes

    DOI: 10.1371/journal.pone.0250663

    PMID: 33905439

    Sample: Cell lysate
  5. RHEUMATOLOGY INTERNATIONAL (2022) IF: 3.58
    Assessment of semaphorin 3A and semaphorin 7A levels in primary Sjogren's syndrome

    DOI: 10.1007/s00296-022-05197-y

    PMID: 36030414

    Sample: serum
  6. INTERNATIONAL OPHTHALMOLOGY (2022) IF: 2.029
    Evaluation of serum semaphorin 3A and interleukin 6 levels in patients with pseudoexfoliation syndrome

    DOI: 10.1007/s10792-022-02599-6

    Sample: serum
  7. GENE (2017) IF: 2.415
    Decreased expression of Sema3A, an immune modulator, in blood sample of multiple sclerosis patients

    DOI: 10.1016/j.gene.2017.02.013

    Sample: Serum
  8. International Journal of Endocrinology (2022) IF: 2.803
    Identification and Functional Characterization of a Novel Variant in the SEMA3A Gene in a Chinese Family with Kallmann Syndrome

    DOI: 10.1155/2022/2504660

    Sample: COS-7 cells
  9. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS (2016) IF: 2.881
    SEMA3A suspended in matrigel improves titanium implant fixation in ovariectomized rats

    DOI: 10.1002/jbm.b.33730

    Sample: Others
  10. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology (2018) IF: 1.373
    The Neuropilin‐1 Ligand, Sema3A, Acts as a Tumor Suppressor in the Pathogenesis of Acute Leukemia

    DOI: 10.1002/ar.24016

    Sample: Plasma
  11. Iranian Journal of Medical Sciences (2019)
    Negative Regulation of Semaphorin-3A Expression in Peripheral Blood Mononuclear Cells Using MicroRNA-497-5p

    DOI: 10.30476/IJMS.2019.44955

    PMID: 31439976

    Sample: Cell culture supernatant
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