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Human sRANKL(Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit

  • Cat.No.:E-EL-H5558

  • Reactivity: Human

To Purchase E-EL-H5558

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human sRANKL in samples. No significant cross-reactivity or interference between Human sRANKL and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human sRANKL concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sRANKL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sRANKL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sRANKL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sRANKL. You can calculate the concentration of Human sRANKL in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human sRANKL were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human sRANKL were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 52.28 122.42 372.33 55.30 121.01 370.30
Standard deviation 3.20 5.66 17.61 3.65 5.14 16.11
CV (%) 6.12 4.62 4.73 6.60 4.25 4.35


The recovery of Human sRANKL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-104 99
EDTA plasma (n=8) 88-101 93
Cell culture media (n=8) 92-102 97


Samples were spiked with high concentrations of Human sRANKL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   O14788
Research AreaCancer, Cardiovascular, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Frontiers in Oncology (2021) IF: 6.244
    Single-Cell Transcriptomics Reveals the Complexity of the Tumor Microenvironment of Treatment-Naive Osteosarcoma

    DOI: 10.3389/fonc.2021.709210

    PMID: 34367994

    Sample: MG63 cells,U-2 OS cells,Saos-2 cells, 143B cells,K
  2. Frontiers in Pharmacology (2020) IF: 4.225
    Corydalis Saxicola Bunting Total Alkaloids Attenuate Walker 256-Induced Bone Pain and Osteoclastogenesis by Suppressing RANKL-Induced NF-κB and c-Fos/NFATc1 Pathways in Rats

    DOI: 10.3389/fphar.2020.609119

    PMID: 33574755

    Sample: serum,cell culture media
    BML-111 inhibits osteoclast differentiation by suppressing the MAPK and NF-κB pathways, alleviating deterioration of the knee joints in a CIA rat model

    DOI: 10.1002/cbin.11990

    Sample: serum
  4. World Journal of Stem Cells (2020) IF: 3.231
    CR6-interacting factor-1 contributes to osteoclastogenesis by inducing receptor activator of nuclear factor κB ligand after radiation

    DOI: 10.4252/wjsc.v12.i3.222

    Sample: H-BM-MSCs
  5. Journal of Immunology Research (2019) IF: 3.404
    Assessment of Salivary Levels of RANKL and OPG in Aggressive versus Chronic Periodontitis

    DOI: 10.1155/2019/6195258

    Sample: saliva
  6. CYTOKINE (2018) IF: 3.514
    Evaluation of gingival crevicular fluid and peri-implant crevicular fluid levels of sclerostin, TWEAK, RANKL and OPG

    DOI: 10.1016/j.cyto.2018.10.021

    PMID: 30377055

    Sample: Gingival Crevicular Fluid
  7. American Journal of Translational Research (2023) IF: 3.94
    Effects of joint irrigation combined with ozone injection on bone metabolism, inflammatory factors, and joint function in knee osteoarthritis.

    PMID: 36777823

    Sample: serum
  8. JOURNAL OF ENDODONTICS (2019) IF: 2.833
    Effect of Calcium Hydroxide Alone or in Combination with Ibuprofen and Ciprofloxacin on Nuclear Factor Kappa B Ligand and Osteoprotegerin Level in Periapical Lesions: A Randomized Controlled Clinical Study

    DOI: 10.1016/j.joen.2019.09.011

    Sample: interstitial fluid
    OPG/RANK/RANKL Axis in Egyptian Children With Acute Lymphoblastic Leukemia After Maintenance Therapy: Relationship to Bone Mineral and Vitamin D Status

    DOI: 10.1097/MPH.0000000000002603

    Sample: serum
  10. International Journal of Lower Extremity Wounds (2019) IF: 1.277
    RANKL Gene Polymorphism as a Potential Biomarker to Identify Acute Charcot Foot Among Indian Population With Type 2 Diabetes: A Preliminary Report:

    DOI: 10.1177/1534734619859730

    Sample: serum
    Sera of patients with axial spondyloarthritis (axSpA) enhance osteoclastogenic potential of monocytes isolated from healthy individuals

    DOI: 10.1186/s12891-018-2356-4

    PMID: 30522483

    Sample: Serum
  12. Journal of Oral Science (2017) IF: 0.876
    Assessment of local and systemic 25-hydroxy-vitamin D, RANKL, OPG, and TNF levels in patients with rheumatoid arthritis and periodontitis

    DOI: 10.2334/josnusd.16-0677

    Sample: Gingival crevicular fluid,Serum
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