×

Human sRANKL(Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit

  • Cat.No.:E-EL-H5558

  • Reactivity: Human

To Purchase E-EL-H5558

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human sRANKL in samples. No significant cross-reactivity or interference between Human sRANKL and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human sRANKL concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sRANKL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sRANKL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sRANKL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sRANKL. You can calculate the concentration of Human sRANKL in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human sRANKL were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human sRANKL were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 52.28 122.42 372.33 55.30 121.01 370.30
Standard deviation 3.20 5.66 17.61 3.65 5.14 16.11
CV (%) 6.12 4.62 4.73 6.60 4.25 4.35

Recovery

The recovery of Human sRANKL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-104 99
EDTA plasma (n=8) 88-101 93
Cell culture media (n=8) 92-102 97

Linearity

Samples were spiked with high concentrations of Human sRANKL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   O14788
SynonymsTNFSF11, CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf, TNLG6B, tumor necrosis factor superfamily member 11, TNF superfamily member 11
Research AreaSignaling transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Cytokine (2019) IF: 3.514
    Evaluation of gingival crevicular fluid and peri-implant crevicular fluid levels of sclerostin, TWEAK, RANKL and OPG

    DOI: 10.1016/j.cyto.2018.10.021

    PMID: 30377055

    Sample: Gingival Crevicular Fluid
  2. BMC musculoskeletal disorders (2018) IF: 1.998
    Sera of patients with axial spondyloarthritis (axSpA) enhance osteoclastogenic potential of monocytes isolated from healthy individuals

    DOI: 10.1186/s12891-018-2356-4

    PMID: 30522483

    Sample: Serum
  3. BioRxiv (2019)
    Crif1 Promotes Osteoporosis in Mice after Radiation

    DOI: 10.1101/725408

    PMID: 99999999

    Sample: Serum
  4. Journal of Physics: Conference Series (2018)
    RANKL concentrations in early orthodontic treatment using passive self-ligating and preadjusted edgewise appliance bracket systems

    DOI: 10.1088/1742-6596/1073/4/042002

    PMID: 99999999

    Sample: gingival crevicular fluid
  5. Journal of Oral Science (2017) IF: 0.876
    Assessment Of local And Systemic 25-hydroxy-vitamin D, RANKL, OPG, and TNF Levels In Patients With Rheumatoid Arthritis And Periodontitis.

    DOI: 10.2334/josnusd.16-0677

    PMID: 28904316

    Sample: Gingival crevicular fluid,Serum
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for Free Shipping 24T ELISA Kit
*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
How do you know
Elabscience:

*Captcha: