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Human VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit

Citations(27)Uniprot : P15692

Cat.No.:E-EL-H0111
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human VEGF-A in samples. No significant cross-reactivity or interference between Human VEGF-A and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human VEGF-A concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGF-A. You can calculate the concentration of Human VEGF-A in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human VEGF-A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human VEGF-A were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	94.10	279.80	737.20	92.40	303.90	745.00
Standard deviation	5.50	14.30	34.60	4.80	15.50	31.30
CV (%)	5.84	5.11	4.69	5.19	5.10	4.20

Recovery

The recovery of Human VEGF-A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-105	98
EDTA plasma (n=8)	95-110	101
Cell culture media (n=8)	88-103	96

Linearity

Samples were spiked with high concentrations of Human VEGF-A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P15692
Synonyms	VEGF-A, VEGFA, MVCD1, VPF
Research Area	Cancer, Cardiovascular, Metabolism, Developmental biology, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

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Cancer letters (2020) IF: 7.36

Small extracellular vesicle-bound vascular endothelial growth factor secreted by carcinoma-associated fibroblasts promotes angiogenesis in a bevacizumab-resistant manner

DOI: 10.1016/j.canlet.2020.08.030
PMID: 32860852	Sample: Cell culture supernatant

IF: 6.638

A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility


	Sample: Cell supernatant

Acta Biomaterialia (2019) IF: 6.638

A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility

DOI: 10.1016/j.actbio.2019.05.012
PMID: 31075515	Sample: Cell culture supernatant

Journal of Agricultural and Food Chemistry (2020) IF: 4.192

Chrysin Inhibits Melanoma Tumor Metastasis via Interfering with the FOXM1/β-Catenin Signaling

DOI: 10.1021/acs.jafc.0c03123
PMID: 32797754	Sample: Cell culture medium

Scientific reports (2018) IF: 4.259

Activation of the complement system in an osteosarcoma cell line promotes angiogenesis through enhanced production of growth factors.

DOI: 10.1038/s41598-018-23851-z
PMID: 29615744

Molecular and Cellular Endocrinology (2020) IF: 3.871

Melatonin stimulates VEGF expression in human granulosa-lutein cells: A potential mechanism for the pathogenesis of ovarian hyperstimulation syndrome

DOI: 10.1016/j.mce.2020.110981
PMID: 32791190	Sample: Follicular Fluid

Biological Research (2020) IF: 3.092

REC8 suppresses tumor angiogenesis by inhibition of NF-κB-mediated vascular endothelial growth factor expression in gastric cancer cells

DOI: 10.1186/s40659-020-00307-1
PMID: 32958054	Sample: Cell lysate

Journal of clinical medicine (2020) IF: 3.303

Association of rs699947 (-2578 C/A) and rs2010963 (-634 G/C) Single Nucleotide Polymorphisms of the VEGF Gene, VEGF-A and Leptin Serum Level, and Cardiovascular Risk in Patients with Excess Body Mass: A Case-Control Study

DOI: 10.3390/jcm9020469
PMID: 32046348	Sample: Serum

Acta Diabetologica (2020) IF: 3.418

Association of IGF1 and VEGFA polymorphisms with diabetic retinopathy in Pakistani population

DOI: 10.1007/s00592-019-01407-5
PMID: 31473834	Sample: Serum

Investigational new drugs (2019) IF: 3.502

The rhenium(I)-diselenoether Anticancer Drug Targets ROS, TGF-β1, VEGF-A, and IGF-1 in an in Vitro Experimental Model of Triple-Negative Breast Cancers

DOI: 10.1007/s10637-019-00727-1
PMID: 30632005	Sample: Cell culture medium

Biomedicine & Pharmacotherapy (2019) IF: 3.457

Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro

DOI: 10.1016/j.biopha.2019.108706
PMID: 30844656	Sample: Wound drainage fluid

Colloids and Surfaces B: Biointerfaces (2018) IF: 3.997

The effects of biomimetically conjugated VEGF on osteogenesis and angiogenesis of MSCs (human and rat) and HUVECs co-culture models.

DOI: 10.1016/j.colsurfb.2018.04.060
PMID: 29730577	Sample: VEGF solution after immobilization

Life sciences (2018) IF: 3.234

The relationship of plasma miR-503 and coronary collateral circulation in patients with coronary artery disease.

DOI: 10.1016/j.lfs.2018.06.001
PMID: 29870767	Sample: Plasma

Cellular Signalling (2017) IF: 3.937

Identification Of NCK1 As A Novel Downstream Effector Of STAT3 In Colorectal Cancer Metastasis And Angiogenesis.

DOI: 10.1016/j.cellsig.2017.04.020
PMID: 28455144	Sample: Cell culture supernatant

Toxicology (2016) IF: 3.582

NLRC5 Promotes Cell Proliferation Via Regulating The AKT/VEGF-A Signaling Pathway In Hepatocellular Carcinoma.

DOI: 10.1016/j.tox.2016.06.012
PMID: 27338800	Sample: Cell culture supernatant

BioMed research international (2018) IF: 2.583

Future Prospective and Current Trend of Biomaterials and Growth Factor Used for Maxillofacial Hard and Soft Tissue Reconstruction

DOI: 10.1155/2018/3249653
PMID: 30581851	Sample: Error information

IF: 2.589

In vitro photodynamic therapy of endothelial cells using hematoporphyrin monomethyl ether (Hemoporfin): Relevance to treatment of port wine stains


	Sample: Cell supernatant

Photodiagnosis and Photodynamic Therapy (2019) IF: 2.589

In vitro photodynamic therapy of endothelial cells using hematoporphyrin monomethyl ether (Hemoporfin): Relevance to treatment of port wine stains

DOI: 10.1016/j.pdpdt.2019.06.003
PMID: 31185325	Sample: Cell culture supernatant

BioMed Research International (2016) IF: 2.476

Reduced Plasma miR-146a Is A Predictor Of Poor Coronary Collateral Circulation In Patients With Coronary Artery Disease.

DOI: 10.1155/2016/4285942
PMID: 28050558	Sample: Plasma

Life Sciences (2017) IF: 2.936

The Relationship Of Plasma Decoy Receptor 3 And Coronary Collateral Circulation In Patients With Coronary Artery Disease.

DOI: 10.1016/j.lfs.2017.09.025
PMID: 28942284	Sample: Plasma

International Journal of General Medicine (2016) IF: 1.927

Thicker carotid intima-media thickness and increased plasma VEGF levels suffered by post-acute thrombotic stroke patients

DOI: 10.2147/IJGM.S114577
PMID: 28008280	Sample: Plasma

Growth Factors (2018) IF: 1.8

Evaluation of the growth factors VEGF-a and VEGF-B in the vitreous and serum of patients with macular and retinal vascular diseases.

DOI: 10.1080/08977194.2018.1477140
PMID: 29969324	Sample: Serum

Journal of Ocular Pharmacology And Therapeutics (2017) IF: 1.679

The Antiangiogenesis Effect Of Pirfenidone In Wound Healing In Vitro.

DOI: 10.1089/jop.2017.0007
PMID: 28933986	Sample: Cell culture supernatant

JMSCR (2018)

Evaluation of Cytokines Response in Plasmodium falciparum Infection among Adolescents in Port Harcourt, Nigeria

DOI: 10.18535/jmscr/v6i9.54
	Sample: Plasma

International Journal of Biological and Chemical Sciences (2020)

Level of VEGF-A and interleukin 6 in lacrimal fluid of patients with diabetic retinopathy

DOI: 10.4314/ijbcs.v14i3.2
	Sample: Tears

Open Journal of Ophthalmology (2020)

Relationship between Angiogenic and Inflammatory Biomarkers and Diabetic Retinopathy

DOI: 10.4236/ojoph.2020.101006
	Sample: Serum

Clinical Nutrition Experimental (2019)

Growth factors profile in conditioned medium human adipose tissue-derived mesenchymal stem cells (CM-hATMSCs)

DOI: 10.1016/j.yclnex.2019.01.002
	Sample: Cell culture medium

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