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Human VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit

  • Cat.No.:E-EL-H0111

  • Reactivity: Human

To Purchase E-EL-H0111

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human VEGF-A in samples. No significant cross-reactivity or interference between Human VEGF-A and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human VEGF-A concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGF-A. You can calculate the concentration of Human VEGF-A in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human VEGF-A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human VEGF-A were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 94.10 279.80 737.20 92.40 303.90 745.00
Standard deviation 5.50 14.30 34.60 4.80 15.50 31.30
CV (%) 5.84 5.11 4.69 5.19 5.10 4.20

Recovery

The recovery of Human VEGF-A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-105 98
EDTA plasma (n=8) 95-110 101
Cell culture media (n=8) 88-103 96

Linearity

Samples were spiked with high concentrations of Human VEGF-A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P15692
SynonymsVEGF-A, VEGFA, MVCD1, VPF
Research AreaCancer,Cardiovascular,Signaling transduction,Metabolism

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Cancer letters (2020) IF: 7.36
    Small extracellular vesicle-bound vascular endothelial growth factor secreted by carcinoma-associated fibroblasts promotes angiogenesis in a bevacizumab-resistant manner

    DOI: 10.1016/j.canlet.2020.08.030

    PMID: 32860852

    Sample: Cell culture supernatant
  2. IF: 6.638
    A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility
    Sample: Cell supernatant
  3. Acta Biomaterialia (2019) IF: 6.638
    A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility

    DOI: 10.1016/j.actbio.2019.05.012

    PMID: 31075515

    Sample: Cell culture supernatant
  4. Journal of Agricultural and Food Chemistry (2020) IF: 4.192
    Chrysin Inhibits Melanoma Tumor Metastasis via Interfering with the FOXM1/β-Catenin Signaling

    DOI: 10.1021/acs.jafc.0c03123

    PMID: 32797754

    Sample: Cell culture medium
  5. Scientific reports (2018) IF: 4.259
    Activation of the complement system in an osteosarcoma cell line promotes angiogenesis through enhanced production of growth factors.

    DOI: 10.1038/s41598-018-23851-z

    PMID: 29615744

  6. Molecular and Cellular Endocrinology (2020) IF: 3.871
    Melatonin stimulates VEGF expression in human granulosa-lutein cells: A potential mechanism for the pathogenesis of ovarian hyperstimulation syndrome

    DOI: 10.1016/j.mce.2020.110981

    PMID: 32791190

    Sample: Follicular Fluid
  7. Biological Research (2020) IF: 3.092
    REC8 suppresses tumor angiogenesis by inhibition of NF-κB-mediated vascular endothelial growth factor expression in gastric cancer cells

    DOI: 10.1186/s40659-020-00307-1

    PMID: 32958054

    Sample: Cell lysate
  8. Journal of clinical medicine (2020) IF: 3.303
    Association of rs699947 (-2578 C/A) and rs2010963 (-634 G/C) Single Nucleotide Polymorphisms of the VEGF Gene, VEGF-A and Leptin Serum Level, and Cardiovascular Risk in Patients with Excess Body Mass: A Case-Control Study

    DOI: 10.3390/jcm9020469

    PMID: 32046348

    Sample: Serum
  9. Acta Diabetologica (2020) IF: 3.418
    Association of IGF1 and VEGFA polymorphisms with diabetic retinopathy in Pakistani population

    DOI: 10.1007/s00592-019-01407-5

    PMID: 31473834

    Sample: Serum
  10. Investigational new drugs (2019) IF: 3.502
    The rhenium(I)-diselenoether Anticancer Drug Targets ROS, TGF-β1, VEGF-A, and IGF-1 in an in Vitro Experimental Model of Triple-Negative Breast Cancers

    DOI: 10.1007/s10637-019-00727-1

    PMID: 30632005

    Sample: Cell culture medium
  11. Biomedicine & Pharmacotherapy (2019) IF: 3.457
    Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro

    DOI: 10.1016/j.biopha.2019.108706

    PMID: 30844656

    Sample: Wound drainage fluid
  12. Colloids and Surfaces B: Biointerfaces (2018) IF: 3.997
    The effects of biomimetically conjugated VEGF on osteogenesis and angiogenesis of MSCs (human and rat) and HUVECs co-culture models.

    DOI: 10.1016/j.colsurfb.2018.04.060

    PMID: 29730577

    Sample: VEGF solution after immobilization
  13. Life sciences (2018) IF: 3.234
    The relationship of plasma miR-503 and coronary collateral circulation in patients with coronary artery disease.

    DOI: 10.1016/j.lfs.2018.06.001

    PMID: 29870767

    Sample: Plasma
  14. Cellular Signalling (2017) IF: 3.937
    Identification Of NCK1 As A Novel Downstream Effector Of STAT3 In Colorectal Cancer Metastasis And Angiogenesis.

    DOI: 10.1016/j.cellsig.2017.04.020

    PMID: 28455144

    Sample: Cell culture supernatant
  15. Toxicology (2016) IF: 3.582
    NLRC5 Promotes Cell Proliferation Via Regulating The AKT/VEGF-A Signaling Pathway In Hepatocellular Carcinoma.

    DOI: 10.1016/j.tox.2016.06.012

    PMID: 27338800

    Sample: Cell culture supernatant
  16. BioMed research international (2018) IF: 2.583
    Future Prospective and Current Trend of Biomaterials and Growth Factor Used for Maxillofacial Hard and Soft Tissue Reconstruction

    DOI: 10.1155/2018/3249653

    PMID: 30581851

    Sample: Error information
  17. IF: 2.589
    In vitro photodynamic therapy of endothelial cells using hematoporphyrin monomethyl ether (Hemoporfin): Relevance to treatment of port wine stains
    Sample: Cell supernatant
  18. Photodiagnosis and Photodynamic Therapy (2019) IF: 2.589
    In vitro photodynamic therapy of endothelial cells using hematoporphyrin monomethyl ether (Hemoporfin): Relevance to treatment of port wine stains

    DOI: 10.1016/j.pdpdt.2019.06.003

    PMID: 31185325

    Sample: Cell culture supernatant
  19. BioMed Research International (2016) IF: 2.476
    Reduced Plasma miR-146a Is A Predictor Of Poor Coronary Collateral Circulation In Patients With Coronary Artery Disease.

    DOI: 10.1155/2016/4285942

    PMID: 28050558

    Sample: Plasma
  20. Life Sciences (2017) IF: 2.936
    The Relationship Of Plasma Decoy Receptor 3 And Coronary Collateral Circulation In Patients With Coronary Artery Disease.

    DOI: 10.1016/j.lfs.2017.09.025

    PMID: 28942284

    Sample: Plasma
  21. International Journal of General Medicine (2016) IF: 1.927
    Thicker carotid intima-media thickness and increased plasma VEGF levels suffered by post-acute thrombotic stroke patients

    DOI: 10.2147/IJGM.S114577

    PMID: 28008280

    Sample: Plasma
  22. Growth Factors (2018) IF: 1.8
    Evaluation of the growth factors VEGF-a and VEGF-B in the vitreous and serum of patients with macular and retinal vascular diseases.

    DOI: 10.1080/08977194.2018.1477140

    PMID: 29969324

    Sample: Serum
  23. Journal of Ocular Pharmacology And Therapeutics (2017) IF: 1.679
    The Antiangiogenesis Effect Of Pirfenidone In Wound Healing In Vitro.

    DOI: 10.1089/jop.2017.0007

    PMID: 28933986

    Sample: Cell culture supernatant
  24. JMSCR (2018)
    Evaluation of Cytokines Response in Plasmodium falciparum Infection among Adolescents in Port Harcourt, Nigeria

    DOI: 10.18535/jmscr/v6i9.54

    PMID: 99999999

    Sample: Plasma
  25. International Journal of Biological and Chemical Sciences (2020)
    Level of VEGF-A and interleukin 6 in lacrimal fluid of patients with diabetic retinopathy

    DOI: 10.4314/ijbcs.v14i3.2

    PMID: 99999999

    Sample: Tears
  26. Open Journal of Ophthalmology (2020)
    Relationship between Angiogenic and Inflammatory Biomarkers and Diabetic Retinopathy

    DOI: 10.4236/ojoph.2020.101006

    PMID: 99999999

    Sample: Serum
  27. Clinical Nutrition Experimental (2019)
    Growth factors profile in conditioned medium human adipose tissue-derived mesenchymal stem cells (CM-hATMSCs)

    DOI: 10.1016/j.yclnex.2019.01.002

    PMID: 99999999

    Sample: Cell culture medium
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