Current Position:Home>>Kits>>ELISA Kits>> Human VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit
Cat.No.:E-EL-H0111
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 31.25-2000 pg/mL |
Sensitivity | 18.75 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human VEGF-A in samples. No significant cross-reactivity or interference between Human VEGF-A and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human VEGF-A concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human VEGF-A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human VEGF-A were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 94.10 | 279.80 | 737.20 | 92.40 | 303.90 | 745.00 |
Standard deviation | 5.50 | 14.30 | 34.60 | 4.80 | 15.50 | 31.30 |
CV (%) | 5.84 | 5.11 | 4.69 | 5.19 | 5.10 | 4.20 |
The recovery of Human VEGF-A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 92-105 | 98 |
EDTA plasma (n=8) | 95-110 | 101 |
Cell culture media (n=8) | 88-103 | 96 |
Samples were spiked with high concentrations of Human VEGF-A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P15692
|
Synonyms | VEGF-A, VEGFA, MVCD1, VPF |
Research Area | Cancer, Cardiovascular, Metabolism, Developmental biology, Signal transduction |
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1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
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PMID: 32860852 |
Sample: Cell culture supernatant |
Sample: Cell supernatant |
PMID: 31075515 |
Sample: Cell culture supernatant |
PMID: 32797754 |
Sample: Cell culture medium |
PMID: 29615744 |
PMID: 32791190 |
Sample: Follicular Fluid |
PMID: 32958054 |
Sample: Cell lysate |
DOI: 10.3390/jcm9020469 |
|
PMID: 32046348 |
Sample: Serum |
PMID: 31473834 |
Sample: Serum |
PMID: 30632005 |
Sample: Cell culture medium |
PMID: 30844656 |
Sample: Wound drainage fluid |
PMID: 29730577 |
Sample: VEGF solution after immobilization |
PMID: 29870767 |
Sample: Plasma |
PMID: 28455144 |
Sample: Cell culture supernatant |
PMID: 27338800 |
Sample: Cell culture supernatant |
DOI: 10.1155/2018/3249653 |
|
PMID: 30581851 |
Sample: Error information |
Sample: Cell supernatant |
PMID: 31185325 |
Sample: Cell culture supernatant |
DOI: 10.1155/2016/4285942 |
|
PMID: 28050558 |
Sample: Plasma |
PMID: 28942284 |
Sample: Plasma |
DOI: 10.2147/IJGM.S114577 |
|
PMID: 28008280 |
Sample: Plasma |
PMID: 29969324 |
Sample: Serum |
PMID: 28933986 |
Sample: Cell culture supernatant |
Sample: Plasma |
Sample: Tears |
Sample: Serum |
Sample: Cell culture medium |