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Hydrogen Peroxide (H2O2) Colorimetric Assay Kit

  • Cat.No.:E-BC-K102-M

  • Detection instrument: Microplate reader(402-407 nm,optimum wavelength: 405 nm)

To Purchase E-BC-K102-M

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  • 96T
  • 48T
Price: $150
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Protocols for metabolism assays

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Detection principle

Hydrogen peroxide can react with ammonium molybdate to form a yellow complex which has a maximum absorption peak at 405 nm. H2O2 content can be calculated by measuring the absorbance value at 405 nm.

Performance characteristics

Synonyms H2O2
Sample type Serum,plasma,urine,tissue,cells
Sensitivity 0.41 mmol/L
Detection range 0.41-125 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 30 min
Precision Average inter-assay CV: 3.600%Average intra-assay CV: 3.200%
Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

Electrical stimulation for more than 8 hours could lead to a significant increase of H2O2 in the media.

S Du et al investigate the promotion effect of wounds healing using programmed electric field together with minocycline. Hydrogen peroxide (H2O2) level in bacteria NB media was determined using H2O2 colorimetric assay kit (E-BC-K102-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.41-125 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Rat serum

1

Mouse serum

1

Mouse plasma

1

Porcine serum

1

Human urine

1

Cell homogenate

1

10% Plant tissue homogenate

1

10% Rat heart tissue homogenate

1

10% Rat brain tissue homogenate

1

10% Rat kidney tissue homogenate

1

10% Rat liver tissue homogenate

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. Journal of Plant Interactions (2018) IF: 1.655
    Brevibacillus sp. promotes maize root colonization by Acaulospora tuberculata and the alteration of associated plant protein responses

    DOI: 10.1080/17429145.2018.1547844

    Sample: Maize Roots
  2. JOURNAL OF PHYSICS D-APPLIED PHYSICS (2021) IF: 3.207
    Effect of pulsed electric field on diabetes-induced glycated enzyme, oxidative stress, and inflammatory markers in vitro and in vivo

    DOI: 10.1088/1361-6463/ac2530

    Sample: Serum
  3. PLANT PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 3.72
    Overexpression of ethylene response factor ERF96 gene enhances selenium tolerance in Arabidopsis

    DOI: 10.1016/j.plaphy.2020.02.024

    PMID: 32097848

    Sample: Plant Tissue
  4. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
    Evaluation of testicular torsion management in Ogbomoso, South-Western Nigeria and surgical detorsion-augmented treatment with phytochemical fractions of Corchorus olitorius leaf in experimental rats

    DOI: 10.1016/j.sjbs.2022.103495

    Sample: Testicular Tissue
  5. Drug Design Development and Therapy (2022) IF: 4.319
    The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study

    DOI: 10.2147/DDDT.S344247

    PMID: 35965961

    Sample: Serum
  6. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556
    Nox2 Upregulation and p38α MAPK Activation in Right Ventricular Hypertrophy of Rats Exposed to Long-Term Chronic Intermittent Hypobaric Hypoxia

    DOI: 10.3390/ijms21228576

    PMID: 33202984

    Sample: Tissue Homogenate
  7. Frontiers in Plant Science (2022) IF: 6.627
    Halotolerant Bacillus altitudinis WR10 improves salt tolerance in wheat via a multi-level mechanism

    DOI: 10.3389/fpls.2022.941388

    PMID: 35909740

    Sample: Roots Or Shoots
  8. PLANT CELL AND ENVIRONMENT (2022) IF: 7.228
    The rice heavy-metal transporter OsNRAMP1 regulates disease resistance by modulating ROS homoeostasis

    DOI: 10.1111/pce.14263

    PMID: 35040151

    Sample: Leaf
  9. MOLECULAR THERAPY (2020) IF: 8.986
    miR-204-3p/Nox4 Mediates Memory Deficits in a Mouse Model of Alzheimer’s Disease

    DOI: 10.1016/j.ymthe.2020.09.006

    Sample: Hippocampus
  10. PHARMACOLOGICAL RESEARCH (2022) IF: 10.334
    Sodium-Glucose Cotransporter-2 (SGLT2) expression in diabetic and non-diabetic failing human cardiomyocytes

    DOI: 10.1016/j.phrs.2022.106448

    PMID: 36096423

    Sample: Ac16 Cell
  11. Journal of Advanced Research (2022) IF: 10.479
    Cadmium accumulation regulated by a rice heavy-metal importer is harmful for host plant and leaf bacteria

    DOI: 10.1016/j.jare.2022.05.010

    PMID: 35640876

    Sample: Leaf
  12. Nano Energy (2021) IF: 17.881
    Surface-engineered triboelectric nanogenerator patches with drug loading and electrical stimulation capabilities: Toward promoting infected wounds healing

    DOI: 10.1016/j.nanoen.2021.106004

    Sample: Nutrient Broth
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