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Cat.No.:E-BC-K102-M
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Synonyms | H2O2 |
Sample type | Serum,plasma,urine,tissue,cells |
Sensitivity | 0.41 mmol/L |
Detection range | 0.41-125 mmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 30 min |
Precision | Average inter-assay CV: 3.600%Average intra-assay CV: 3.200% |
Other instruments required | Micropipettor, Centrifuge, Incubator, Vortex mixer |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
Electrical stimulation for more than 8 hours could lead to a significant increase of H2O2 in the media.
S Du et al investigate the promotion effect of wounds healing using programmed electric field together with minocycline. Hydrogen peroxide (H2O2) level in bacteria NB media was determined using H2O2 colorimetric assay kit (E-BC-K102-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.41-125 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Rat serum |
1 |
Mouse serum |
1 |
Mouse plasma |
1 |
Porcine serum |
1 |
Human urine |
1 |
Cell homogenate |
1 |
10% Plant tissue homogenate |
1 |
10% Rat heart tissue homogenate |
1 |
10% Rat brain tissue homogenate |
1 |
10% Rat kidney tissue homogenate |
1 |
10% Rat liver tissue homogenate |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Sample: Maize Roots |
Sample: Serum |
PMID: 32097848 |
Sample: Plant Tissue |
Sample: Testicular Tissue |
DOI: 10.2147/DDDT.S344247 |
|
PMID: 35965961 |
Sample: Serum |
DOI: 10.3390/ijms21228576 |
|
PMID: 33202984 |
Sample: Tissue Homogenate |
PMID: 35909740 |
Sample: Roots Or Shoots |
DOI: 10.1111/pce.14263 |
|
PMID: 35040151 |
Sample: Leaf |
Sample: Hippocampus |
PMID: 36096423 |
Sample: Ac16 Cell |
PMID: 35640876 |
Sample: Leaf |
Sample: Nutrient Broth |