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Hydrogen Peroxide (H2O2) Fluorometric Assay Kit

Cat.No.:E-BC-F001
Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)

Detection method: Fluorescence method

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Detection principle

In the presence of peroxidase, hydrogen peroxide reacts with the fluorescent probe, and the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm is proportional to the hydrogen peroxide concentration.

Performance characteristics

Sample type	Serum, plasma, tissue, cells
Sensitivity	0.02 μmol/L
Detection range	0.02-10 μmol/L
Detection method	Fluorescence method
Assay type	Quantitative
Assay time	50 min
Precision	Average inter-assay CV: 3.600%Average intra-assay CV: 1.100%
Other instruments required	Micropipettor, Incubator, Vortex mixer, Centrifuge
Storage	Reagent 5 & Reagent 6, 2-8℃; others, -20℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.02-10 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1-3
Rat serum	1-4
Mouse serum	1-4
Mouse plasma	1-2
Porcine serum	1-3
HepG2 cell culture supernatant	1
10% Mouse liver tissue homogenate	1-3
10% Mouse brain tissue homogenate	1-3
10% Rat lung tissue homogenate	1-3
HepG2 cell homogenate (6.44 gprot/L)	1-2

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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