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Hydrogen Peroxide (H2O2) Fluorometric Assay Kit

  • Cat.No.:E-BC-F001

  • Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)

To Purchase E-BC-F001

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Price: $150

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Detection principle

In the presence of peroxidase, hydrogen peroxide reacts with the fluorescent probe, and the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm is proportional to the hydrogen peroxide concentration.

Performance characteristics

Sample type Serum, plasma, tissue, cells
Sensitivity 0.02 μmol/L
Detection range 0.02-10 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 50 min
Precision Average inter-assay CV: 3.6%Average intra-assay CV: 1.1%
Other instruments required Micropipettor, Incubator, Vortex mixer, Centrifuge
Storage Reagent 5 & Reagent 6, 2-8℃; others, -20℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.02-10 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Rat serum


Mouse serum


Mouse plasma


Porcine serum


HepG2 cell culture supernatant


10% Mouse liver tissue homogenate


10% Mouse brain tissue homogenate


10% Rat lung tissue homogenate


HepG2 cell homogenate (6.44 gprot/L)


Note: The diluent is reagent 1.

Ralated product

Cat Number Product Name
E-BC-K102-M Hydrogen Peroxide (H2O2) Colorimetric Assay Kit


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