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Hydrogen Peroxide (H2O2) Fluorometric Assay Kit

Cat:E-BC-F001
Manual MSDS

Price: $ 150

Price: $ 120

Size:
96T 48T
Quantity:
  • Sample Type: serum;plasma;tissue;cells
  • Detection Instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
  • Reasearch Areas: Oxidative stress;Plant Physiology Series;Ferroptosis
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Product Details
Detection Principle In the presence of peroxidase, hydrogen peroxide reacts with the fluorescent probe, and the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm is proportional to the hydrogen peroxide concentration.
Sample Type serum,plasma,tissue,cells
Sensitivity 0.02 μmol/L
Detection Range 0.02-10 μmol/L
Detection Method Fluorescence method
Assay Time 50 min
Precision inter-assay CV: 3.6% | intra-assay CV: 1.1%
Storage This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 1-3
Mouse serum 1-4
Mouse plasma 1-2
Porcine serum 1-3
Rat serum 1-4
HepG2 culture supernatant 1
10% Mouse liver tissue homogenate 1-3
10% Mouse brain tissue homogenate 1-3
10% Rat lung tissue homogenate 1-3
HepG2 cells homogenate (the concentration of protein is 6.44 gprot/L) 1-2
The diluent is buffer solution. For the dilution of other sample types, please do pretest to confirm the dilution factor.