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Hdroxyproline (HYP) Colorimetric Assay Kit

Cat.No.:E-BC-K062-S
Detection instrument: Spectrophotometer (550-570 nm, optimum wavelength: 558 nm)

Detection method: Colorimetric method

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Protocols for metabolism assays

FAQ for metabolism assays

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Detection principle

The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558 nm.

Performance characteristics

Synonyms	HYP
Sample type	serum, animal tissue and urine samples
Sensitivity	0.032 μg/mL
Detection range	0.032-10 μg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	45 min
Precision	Average inter-assay CV: 5.700%Average intra-assay CV: 4.900%
Other instruments required	Vortex mixer, Centrifuge, Water bath
Other reagents required	6 mol/L Hydrochloric acid, Concentrated hydrochloric acid (12 mol/L), N-propyl alcohol
Storage	2-8℃
Valid period	12 months

Ralated product

Cat Number	Product Name
E-BC-K055-M	Total Amino Acids (T-AA) Colorimetric Assay Kit

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Materials Science & Engineering C-Materials for Biological Applications (2021) IF: 7.328
Development of Si doped nano hydroxyapatite reinforced bilayer chitosan nanocomposite barrier membranes for guided bone regeneration

DOI: 10.1016/j.msec.2021.112298

PMID: 34474849
Sample: NIH/3 T3 cells
COLLOIDS AND SURFACES B-BIOINTERFACES (2023) IF: 5.999
A Multifunctional Sateen Woven Dressings for Treatment of Skin Injuries

DOI: 10.1016/j.colsurfb.2023.113197

Sample: human adipose tissue-derived mesenchymal stem cell
INFLAMMATION (2021) IF: 4.092
Simultaneous Modulation of NLRP3 Inflammasome and Nrf2/ARE Pathway Rescues Thioacetamide-Induced Hepatic Damage in Mice: Role of Oxidative Stress and Inflammation

DOI: 10.1007/s10753-021-01571-3

PMID: 34664134
Sample: liver
Biomedical Materials (2021) IF: 3.715
Bioactive snail mucus-slime extract loaded chitosan scaffolds for hard tissue regeneration: the effect of mucoadhesive and antibacterial extracts on physical characteristics and bioactivity of chitosan matrix

DOI: 10.1088/1748-605X/ac2352

Sample: SW1353 cell,Saos-2 cell

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Reviews/Q&A

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Reviews
Q&A

N*****************mSubmitted [ Feb 07 2023 ]

Asked: Hi, Can I use this assay to test samples consisting of Collagen at a concentration of 3mg/mL? If not, is it possible to dilute the sample and perform the assay? Also, is the assay compatible for tissue samples. Can you please share the assay manual.

adminSubmitted [ Feb 07 2023 ]

Answered: Thanks for your question. We noticed that your sample is very special, but the concentration is higher than the sensitivity of the kit. Theoretically this kit is compatible for the sample you mentioned. Please do a pre-test before your formal experiment. And we would email you the manual for your information. Have a nice day!

M**********dSubmitted [ Aug 30 2022 ]

Asked: Do I have to finish the whole process in one day? As the sample preparation takes long time.

adminSubmitted [ Aug 30 2022 ]

Answered: You don't have to. The sample can be stored at 4 degree overnight after neutralized to pH 6.5-7.0.

D********dSubmitted [ Aug 05 2022 ]

Asked: How long does the whole process take?

adminSubmitted [ Aug 05 2022 ]

Answered: The operation time is half an hour. The preparation time of reagents varies from person to person. If the operation is fast, it can be completed within half an hour; the sample processing time and sample type are related to the specific part of the manual.

G************rSubmitted [ Jul 27 2018 ]

Asked: I am a research scholar in department of pharmaceutical sciences and drug research; Punjabi University,Patiala; Punjab;(India). I need hydroxyproline kit for collagen estimation in experimental animals. please send me a quotation for the same.

adminSubmitted [ Jul 27 2018 ]

Answered: Dear Sir, Greetings from Elabscience~ I am Marina from Elabscience, the sales for India. There are two kits for hydroxyproline kit as below, E-BC-K572 Hydroxyproline (HYP) Colorimetric Assay Kit Spectrophotometry or Microplate reader E-BC-K061 Hydroxyproline Colorimetric Assay Kit (Alkali hydrolysis method) Spectrophotometry If you need the manuals pls contact with me for email Any further assistance,pls feel free to contact me again. Thanks and best wishes,

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