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IL17RA Polyclonal Antibody

Cat:E-AB-70147
Manual MSDS

Price: $ 410

Price: $ 260

Price: $ 160

Size:
200μL 120μL 60μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB
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Product Details
Verified Samples Verified Samples in WB:Hela,Jurkat,Mouse lung,Mouse colon,Rat colon
Dilution

WB 1:500-1:1000

Western Blot Operation Guide
Clonality Polyclonal
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse IL17RA
Abbre IL17RA
Synonyms CANDF5;CD217;CD217 antigen;CDw217;CTLA8;HIL 17R;hIL17R;I17RA;IL 17 receptor A;IL 17 receptor;IL 17RA;IL-17 receptor A;IL-17RA;IL17;IL17A receptor;IL17R;IL17RA;IMD51;Interleukin 17 receptor A;Interleukin-17 receptor A;MGC10262
Swissprot
Calculated MW 96kDa
Observed MW 96kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Membrane.
Concentration 0.96 mg/mL
Buffer PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer; Immunology
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The transmembrane protein encoded by this gene (interleukin 17A receptor; IL17RA) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Alternative splicing results in multiple transcript variants encoding different isoforms.