IL17RA Polyclonal Antibody
Price: $ 410
Price: $ 260
Price: $ 160
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: WB
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:Hela,Jurkat,Mouse lung,Mouse colon,Rat colon |
Dilution |
WB 1:500-1:1000 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse IL17RA |
Abbre | IL17RA |
Synonyms | CANDF5;CD217;CD217 antigen;CDw217;CTLA8;HIL 17R;hIL17R;I17RA;IL 17 receptor A;IL 17 receptor;IL 17RA;IL-17 receptor A;IL-17RA;IL17;IL17A receptor;IL17R;IL17RA;IMD51;Interleukin 17 receptor A;Interleukin-17 receptor A;MGC10262 |
Swissprot | |
Calculated MW | 96kDa |
Observed MW |
96kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Membrane. |
Concentration | 0.96 mg/mL |
Buffer | PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4 |
Purification Method | Affinity purification |
Research Areas | Cancer; Immunology |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The transmembrane protein encoded by this gene (interleukin 17A receptor; IL17RA) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Alternative splicing results in multiple transcript variants encoding different isoforms. |