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IL6 Polyclonal Antibody

Cat:E-AB-30095 Citations (3)
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human
  • Applications: WB;IHC-p
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Product Details
Verified Samples Verified Samples in WB:K562
Verified Samples in IHC:Human lung
Dilution

WB 1:500-1:2000, IHC 1:100-1:300

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthesized peptide derived from the Internal region of human IL-6.
Abbre IL-6
Synonyms Interleukin BSF 2;B cell differentiation factor;B cell stimulatory factor 2;B-cell stimulatory factor 2;BSF 2;BSF-2;BSF2;CDF;CTL differentiation factor;Hepatocyte stimulatory factor;HGF;HSF;Hybridoma growth factor;Hybridoma growth factor Interferon beta-2;IFN-beta-2;IFNB2;IL 6;IL-6;IL6;IL6;Interferon beta 2;Interferon beta-2;Interleukin 6;Interleukin 6 (interferon beta 2);Interleukin BSF 2;Interleukin-6
Swissprot
Calculated MW 21-30kDa
Observed MW 23kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Secreted.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer; Metabolism; Immunology; Neuroscience
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.