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IP3(Inositol Triphosphate) ELISA Kit

  • Cat.No.:E-EL-0059

  • Reactivity: Universal

To Purchase E-EL-0059

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes IP3 in samples. No significant cross-reactivity or interference between IP3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of IP3 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with IP3. During the reaction, IP3 in the sample or standard competes with a fixed amount of IP3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to IP3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IP3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level IP3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level IP3 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 54.40 130.40 426.20 58.40 132.30 424.50
Standard deviation 2.70 5.20 16.20 4.00 7.70 14.90
CV (%) 4.96 3.99 3.80 6.85 5.82 3.51


The recovery of IP3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-100 93
EDTA plasma(n=8) 88-98 93
Cell culture media(n=8) 85-101 92


Samples were spiked with high concentrations of IP3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsInositol trisphosphate, inositol 1, 4, 5-trisphosphate, triphosphoinositol, InsP3, Ins3P, IP3
Research AreaImmunology, Cancer

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Molecular Cancer (2019) IF: 10.679
    Epigenetically upregulated oncoprotein PLCE1 drives esophageal carcinoma angiogenesis and proliferation via activating the PI-PLCε-NF-κB signaling pathway and VEGF-C/ Bcl-2 expression

    DOI: 10.1186/s12943-018-0930-x

    Sample: Esophageal squamous cell carcinoma (ESCC) cells
  2. Communications Biology (2021) IF: 6.268
    Iron induces two distinct Ca2+ signalling cascades in astrocytes

    DOI: 10.1038/s42003-021-02060-x

    Sample: Cell culture supernatant
  3. Nutrients (2022) IF: 6.706
    Dihydromyricetin Enhances Exercise-Induced GLP-1 Elevation through Stimulating cAMP and Inhibiting DPP-4

    DOI: 10.3390/nu14214583

  4. ENDOCRINOLOGY (2023) IF: 5.051
    Aspirin suppresses hepatic glucagon signaling through decreasing production of thromboxane A2

    DOI: 10.1210/endocr/bqac217

    Sample: Hepatocyte
  5. Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
    The Degree of Helicobacter pylori Infection Affects the State of Macrophage Polarization through Crosstalk between ROS and HIF-1α

    DOI: 10.1155/2020/5281795

    Sample: Cell culture supernatant
    A2A R mediated modulation in IP3 levels altering the [Ca2+]i through cAMP-dependent PKA signalling pathway

    DOI: 10.1016/j.bbagen.2022.130242

    PMID: 36084905

    Sample: HEK 293 cell
  7. FEBS Journal (2019) IF: 4.739
    Impaired calcium homeostasis via advanced glycation end products promotes apoptosis through endoplasmic reticulum stress in human nucleus pulposus cells and exacerbates intervertebral disc degeneration in rats

    DOI: 10.1111/febs.14972

    PMID: 31230413

    Sample: Cell culture supernatant,Tissue homogenate
    A new missense mutation of calcium sensing receptor with isoleucine replaced by serine at codon 857 leading to type V Bartter syndrome

    DOI: 10.1016/j.yexcr.2022.113080

    PMID: 35192837

    Sample: Urine stem cell
  9. Scientific Reports (2020) IF: 3.998
    From extraocular photoreception to pigment movement regulation: a new control mechanism of the lanternshark luminescence

    DOI: 10.1038/s41598-020-67287-w

    PMID: 32576969

    Sample: Tissue homogenate
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