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Iron Colorimetric Assay Kit

  • Cat.No.:E-BC-K139-M

  • Detection instrument: Microplate reader(510-530 nm,optimum wavelength: 520 nm)

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Detection principle

Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly.

Performance characteristics

Synonyms Fe
Sample type Serum,tissue
Sensitivity 0.08 mg/L
Detection range 0.29-10 mg/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 25 min
Precision Average inter-assay CV: 2.8%Average intra-assay CV: 1.2%
Other instruments required Micropipettor, Centrifuge, Water bath, Incubator, Vortex mixer
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.29-10 mg/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Mouse serum


10% Mouse liver tissue homogenate


10% Rat kidney tissue homogenate


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


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