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Iron Colorimetric Assay Kit

Cat.No.:E-BC-K139-M
Detection instrument: Microplate reader(510-530 nm,optimum wavelength: 520 nm)

Detection method: Colorimetric method

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Detection principle

Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly.

Performance characteristics

Synonyms	Fe
Sample type	Serum,tissue
Sensitivity	0.08 mg/L
Detection range	0.29-10 mg/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	25 min
Precision	Average inter-assay CV: 2.800%Average intra-assay CV: 1.200%
Other instruments required	Micropipettor, Centrifuge, Water bath, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

The iron levels in A549 cells were significantly increased after treatment with BPQDs. (*P<0.05, **P<0.01)

F Ruan et al investigate the nanotoxicological assessment of Black Phosphorus Quantum Dots (BPQD). Iron (Fe) level in lung A549 cells were determined using iron colorimetric assay kit (E-BC-K139-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.29-10 mg/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	1
10% Mouse liver tissue homogenate	1
10% Rat kidney tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Small Methods (2021) IF: 14.188
RNA m6A Modification Alteration by Black Phosphorus Quantum Dots Regulates Cell Ferroptosis: Implications for Nanotoxicological Assessment

DOI: 10.1002/smtd.202001045

PMID: 34927824
Sample: Cell sample
Cell Death & Disease (2022) IF: 9.685
UHRF1-mediated ferroptosis promotes pulmonary fibrosis via epigenetic repression of GPX4 and FSP1 genes

DOI: 10.1038/s41419-022-05515-z

Sample: lung tissue
JOURNAL OF CONTROLLED RELEASE (2022) IF: 9.776
A targeted nanomodulator capable of manipulating tumor microenvironment against metastasis

DOI: 10.1016/j.jconrel.2022.06.022

PMID: 35716882
Sample: 4 T1 cell-xenograft
ANTIOXIDANTS & REDOX SIGNALING (2021) IF: 8.401
A novel identified circular RNA, mmu_mmu_circRNA_0000309 involves in Germacrone-mediated the improvement of diabetic nephropathy through regulating ferroptosis by targeting miR-188-3p/GPX4 signaling axis

DOI: 10.1089/ars.2021.0063

Sample: kidney,Primary mouse podocyte MPC5
Cell Death & Disease (2022) IF: 8.4689
STAT6 inhibits ferroptosis and alleviates acute lung injury via regulating P53/SLC7A11 pathway

DOI: 10.1038/s41419-022-04971-x

PMID: 35668064
Sample: lung tissue,HBE cell
Antioxidants (2022) IF: 7.675
Cathelicidin Attenuates Hyperoxia-Induced Lung Injury by Inhibiting Ferroptosis in Newborn Rats

DOI: 10.3390/antiox11122405

Sample: lung tissues
Frontiers in Oncology (2022) IF: 6.244
Sevoflurane Induces Ferroptosis of Glioma Cells Through Activating the ATF4-CHAC1 Pathway.

DOI: 10.3389/fonc.2022.859621

Sample: U251 cell,U87 cell
Antioxidants (2022) IF: 6.313
Hyperoxia Induces Ferroptosis and Impairs Lung Development in Neonatal Mice

DOI: 10.3390/antiox11040641

PMID: 35453326
Sample: lung tissues
Food & Function (2022) IF: 6.317
Arthrospira platensis (Spirulina) fortified functional foods ameliorates iron and protein malnutrition by improving growth, modulating oxidative stress and gut microbiota in rats

DOI: 10.1039/D2FO02226E

Sample: serum
International Journal of Nanomedicine (2022) IF: 6.4
A Composite Deferoxamine/Black Phosphorus Nanosheet/Gelatin Hydrogel Scaffold for Ischemic Tibial Bone Repair

DOI: 10.2147/IJN.S351814

PMID: 35299865
Sample: BPN-DFO hydrogel
Nutrients (2022) IF: 6.706
Iron Depletion in Systemic and Muscle Compartments Defines a Specific Phenotype of Severe COPD in Female and Male Patients: Implications in Exercise Tolerance

DOI: 10.3390/nu14193929

PMID: 36235581
Sample: Muscle
JOURNAL OF ETHNOPHARMACOLOGY (2023) IF: 5.195
Verbenalin attenuates hepatic damage and mitochondrial dysfunction in alcohol-associated steatohepatitis by regulating MDMX/PPARα-mediated ferroptosis

DOI: 10.1016/j.jep.2023.116227

Sample: serum,Liver tissue,AML-12 cell
TOXICOLOGY (2020) IF: 4.099
Inhibition of ferroptosis attenuates busulfan-induced oligospermia in mice

DOI: 10.1016/j.tox.2020.152489

Sample: testicular tissue
BIOLOGY OF REPRODUCTION (2021) IF: 4.285
Chronic overload of concentration-dependent iron exerts different effects on ovarian function in C57BL/6J mice

DOI: 10.1093/biolre/ioab040

Sample: serum，ovary
MOLECULAR IMMUNOLOGY (2021) IF: 4.407
Hydrogen sulfide attenuates ferroptosis and stimulates autophagy by blocking mTOR signaling in sepsis-induced acute lung injury

DOI: 10.1016/j.molimm.2021.12.003

PMID: 34952420
Sample: lung tissue
Bioengineered (2022) IF: 3.269
Amentoflavone promotes ferroptosis by regulating reactive oxygen species (ROS) /5’AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) to inhibit the malignant progression of endometrial carcinoma cells

DOI: 10.1080/21655979.2022.2079256

Sample: KLE cell

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