Iron Colorimetric Assay Kit (E-BC-K139-M)
For research use only.
| Detection Principle | Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly. |
| Synonyms | Fe |
| Sample Type | Serum,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (510-530 nm, optimum wavelength: 520 nm) |
| Research Area | Inorganic Ions , Plant Physiology Series , Ferroptosis |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.08 mg/L |
| Detection Range | 0.29-10 mg/L |
| Precision | inter-assay CV: 2.8% | intra-assay CV: 1.2% |
| Sample Volume | 75 μL |
| Assay Time | 25 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Mouse serum | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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