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KCNJ2 Polyclonal Antibody

Cat:E-AB-18197
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human; Mouse; Rat
  • Applications: WB;IHC
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For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:A549
Verified Samples in IHC:Human liver cancer
Dilution

WB 1:500-1:2000, IHC 1:30-1:150

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthetic peptide of human KCNJ2
Abbre KCNJ2
Synonyms Cardiac inward rectifier potassium channel;HHBIRK 1;HHBIRK1;HHIRK 1;HHIRK1;HIRK 1;hIRK1;Inward rectifier K;Inward rectifier K(+) channel Kir2.1;Inward rectifier potassium channel 2;inwardly rectifying subfamily J member 2;IRK 1;IRK-1;IRK1;IRK2;KCNJ2;KIR2.1;LQT 7;LQT7;Potassium channel;Potassium channel inwardly rectifying subfamily J member 2;Potassium inwardly rectifying channel J2;Potassium inwardly rectifying channel subfamily J member 2;SQT 3;SQT3
Swissprot
Calculated MW 48 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Membrane.
Concentration 0.72 mg/mL
Buffer PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Purification Method Antigen affinity purification
Research Areas Cancer; Neuroscience
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Mutations in this gene have been associated with Andersen syndrome, which is characterized by periodic paralysis, cardiac arrhythmias, and dysmorphic features.