L-Lactic Acid (LA) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
    • Lactic Acid Standard Curve-Elabscience
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    >
    • Biochemical Assay Kit-Elabscience
    • Lactic Acid Standard Curve-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Lactic Acid Standard Curve-Elabscience

      Catalog number:E-BC-K044-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $300
      Can detect 96 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(530 nm)

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      Using NAD+ as hydrogen acceptor, LDH catalyzes the conversion of both lactate and NAD+ into pyruvic acid and NADH respectively. 1-Methoxy-5-methyl phenazine methyl sulfate (PMS) transfers hydrogen from NADH to NBT which deoxidize into purple chromogenic substrate. Lactic acid content can be calculated by measuring the OD value at 530 nm.

      Performance characteristics

      Sample type serum,plasma,cell culture supernatant,tissue,cells
      Sensitivity 0.05 mmol/L
      Detection range 0.05-6.0 mmol/L
      Precision Average inter-assay CV: 1.9%Average intra-assay CV: 1.1%
      Other instruments required Micropipettor, Vortex mixer, Incubator, Centrifuge
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months
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      • Reviews (2)
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      Verified Customer

      p****uSubmitted [ Jun 19 2019 ]

      • Application:Biochemical
      • Description:This kit can be fast, reaction only takes 10 minutes, can accurately get the experimental results, less interference factors. I use this kit to detect human serum, each sample is tested three times, the difference between the results of samples is small, and all samples have a good trend. So the quality of this kit is very good.
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      Verified Customer

      C***ySubmitted [ May 31 2019 ]

      • Application:Biochemical
      • Description:This biochemical kit is good, easy and fast. with a small sample size, but it needs fast detection
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      MSDS for ELISA ISO 9001

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