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Cat.No.:E-BC-K044-M
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Protocols for metabolism assays
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Sample type | Serum,plasma,cell culture supernatant,tissue,cells |
Sensitivity | 0.10 mmol/L |
Detection range | 0.12-7.0 mmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 30 min |
Precision | Average inter-assay CV: 3.500%Average intra-assay CV: 1.400% |
Other instruments required | Micropipettor, Centrifuge, Incubator, Vortex mixer |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
Lactate secretion was significantly increased in HG group. (*P<0.05)
H Mu at al investigate the glucose metabolism that controlled human tumor immunosurveillance in diabetes. L-Lactate (L-LA) of cell supernatant was determined using L-lactate colorimetric assay kit (E-BC-K044-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.12-7.0 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
2-5 |
10% Rat kidney tissue homogenate |
1-3 |
10% Rat brain tissue homogenate |
1 |
HepG2 cell culture supernatant |
1 |
HepG2 cells |
1 |
PMID: 36669474 |
Sample: Serum |
PMID: 35821253 |
Sample: serum |
DOI: 10.7150/thno.62302 |
|
PMID: 34522223 |
Sample: heart tissue |
Sample: microglia |
Sample: H1975 cell |
Sample: lung tissue |
PMID: 35842652 |
Sample: SGC-996 cell,EH-GB1 cell,GBC-SD cell |
Sample: KIJ265T cell,KIJ308T cell,RPTEC/TERT1 cell,Caki-1 |
DOI: 10.1111/cas.15351 |
|
Sample: AGS cell,HGC-27 cell |
PMID: 34646996 |
Sample: THP-1 cell |
Sample: HepG2 cell |
DOI: 10.7150/jca.57926 |
|
Sample: SW480 cell |
Sample: SKBR-3 breast cancer cell |
DOI: 10.1530/REP-20-0381 |
|
PMID: 33112813 |
Sample: testis |
Sample: MCF-7 cell,HepG2 cell,Caco-2 cell |
DOI: 10.1002/hed.27229 |
|
PMID: 36268878 |
Sample: CAL27 cells,HSC3 cells |
PMID: 34217312 |
Sample: Huh-8 cells |
PMID: 35438362 |
Sample: SW480 cell,HCT116 cell |