Pyruvic Acid Colorimetric Assay Kit (E-BC-K130-M)
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For research use only.
| Detection Principle | Pyruvic acid can react with chromogenic agent and the reaction product is reddish brown in alkaline solution. The depth of color is directly proportional to the pyruvate content. The pyruvate content can be calculated by measuring the OD value at 505 nm. |
| Sample Type | Serum,Plasma,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (480-520 nm, optimum wavelength: 505 nm) |
| Research Area | Amino Acids And Proteins , Mitochondria Function , Tricarboxylic Acid（TCA）Cycle , Cuproptosis , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.003 μmol/mL |
| Detection Range | 0.003-2.0 μmol/mL |
| Precision | inter-assay CV: 3.6% | intra-assay CV: 2.3% |
| Sample Volume | 15 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat heart tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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