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Lactase Activity Assay Kit

Cat.No.:E-BC-K131-M
Detection instrument: Microplate reader(495 nm-510 nm,optimum wavelength: 505 nm)

Detection method: Colorimetric method

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96T

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Detection principle

Lactase decomposes lactose to produce glucose. Under the action of enzyme, glucose produces hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide to produce colored substances. Lactase activity can be calculated by measuring the OD value at 505 nm.

Performance characteristics

Sample type	Animal tissue
Sensitivity	3.94 U/mL
Detection range	12.5-2000 U/mL.
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	60 min
Precision	Average inter-assay CV: 8.5%Average intra-assay CV: 4.5%
Other instruments required	Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	Reagent 3: -20℃; Others: 2-8℃
Valid period	6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (12.5-2000 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Rat lleal tissue homogenate	1
10% Rat jejunum tissue homogenate	1
10% Rat liver tissue homogenate	1
10% Rat kidney tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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