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Lactase Activity Assay Kit

  • Cat.No.:E-BC-K131-M

  • Detection instrument: Microplate reader(495 nm-510 nm,optimum wavelength: 505 nm)

To Purchase E-BC-K131-M

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  • 96T
Price: $200
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Detection principle

Lactase decomposes lactose to produce glucose. Under the action of enzyme, glucose produces hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide to produce colored substances. Lactase activity can be calculated by measuring the OD value at 505 nm.

Performance characteristics

Sample type Animal tissue
Sensitivity 3.94 U/mL
Detection range 12.5-2000 U/mL.
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 60 min
Precision Average inter-assay CV: 8.5%Average intra-assay CV: 4.5%
Other instruments required Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage Reagent 3: -20℃; Others: 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (12.5-2000 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Rat lleal tissue homogenate

1

10% Rat jejunum tissue homogenate

1

10% Rat liver tissue homogenate

1

10% Rat kidney tissue homogenate

1

Note: The diluent is normal saline (0.9% NaCl).

Citations

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