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Lactase Activity Assay Kit

Cat:E-BC-K131-M Citations (2)
Manual MSDS

Price: $ 200

Size:
96T
Quantity:
  • Sample Type: animal tissue
  • Detection Instrument: Microplate reader(495 nm-510 nm,optimum wavelength: 505 nm)
  • Reasearch Areas: Glycolysis Lipid Metabolism
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For research use only. Order now, ship in 3 days

Product Details
Detection Principle Lactase decomposes lactose to produce glucose. Under the action of enzyme, glucose produces hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide to produce colored substances. Lactase activity can be calculated by measuring the OD value at 505 nm.
Sample Type animal tissue
Sensitivity 3.94 U/mL
Detection Range 12.5-2000 U/mL.
Detection Method Colorimetric method
Assay Time 60 min
Precision inter-assay CV: 8.5% | intra-assay CV: 4.5%
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
10% Rat lleal tissue homogenate 1
10% Rat jejunum tissue homogenate 1
10% Rat liver tissue homogenate 1
10% Rat kidney tissue homogenate 1
The diluent is normal saline (0.9% NaCl). For the dilution of other sample types, please do pretest to confirm the dilution factor.