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Lactate Dehydrogenase (LDH) Activity Assay Kit

Citations(35)

  • Cat.No.:E-BC-K046-M

  • Detection instrument: Microplate reader(440-460 nm,optimum wavelength: 450 nm)

Manual MSDS

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Detection principle

Using coenzyme I as a hydrogen carrier, LDH catalyze lactic acid to produce pyruvate. Pyruvate reacted with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which was red-brown in alkaline solution, and the color depth was proportional to pyruvate concentration. The activity of LDH could be calculated by measuring OD value.

Performance characteristics

Synonyms LDH
Sample type Serum,plasma,hydrothorax,tissue,cells
Sensitivity 6 U/L
Detection range 6-1000 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 45 min
Precision Average inter-assay CV: 2.400%Average intra-assay CV: 1.800%
Other instruments required Micropipettor, Multichannel pipette, Incubator
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Images

The activity of LDH that shows the membrane leakage was significantly increased after treating with CuO. (**P<0.01 vs. Control, #P<0.05, ###P<0.001 vs. untreated group)

L A Jaragh-Alhadad et al investigates the neurotoxicity of copper oxide that may promote Alzheimer’s diseases. Lactate Dehydrogenase (LDH) activity of human cell was determined using LDH activity assay kit (E-BC-K046-M).

Citations

  1. SCIENCE OF THE TOTAL ENVIRONMENT (2021) IF: 7.963
    Polystyrene microplastics induce hepatotoxicity and disrupt lipid metabolism in the liver organoids

    DOI: 10.1016/j.scitotenv.2021.150328

    PMID: 34571217

    		 Sample: liver organoids (LOs)
  2. FOOD AND CHEMICAL TOXICOLOGY (2022) IF: 6.025
    Procyanidins inhibit zearalenone-induced apoptosis and oxidative stress of porcine testis cells through activation of Nrf2 signaling pathway

    DOI: 10.1016/j.fct.2022.113061

    PMID: 35489465

    		 Sample: pig testicular cell (ST cell)
  3. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022) IF: 6.953
    Copper oxide nanoparticles promote amyloid-β-triggered neurotoxicity through formation of oligomeric species as a prelude to Alzheimer's diseases

    DOI: 10.1016/j.ijbiomac.2022.03.006

    PMID: 35259430

    		 Sample: SH-SY5Y cell
  4. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY (2021) IF: 5.085
    Activation of CD137 signaling promotes macrophage apoptosis dependent on p38 MAPK pathway-mediated mitochondrial fission

    DOI: 10.1016/j.biocel.2021.106003

    PMID: 33971320

    		 Sample: Cell culture supernatant
  5. Food Bioscience (2022) IF: 5.318
    Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

    DOI: 10.1016/j.fbio.2022.102079
    Sample: livers
  6. Foods (2022) IF: 5.561
    Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

    DOI: 10.3390/foods11193048

    PMID: 36230124

    		 Sample: serum
  7. Frontiers in Pharmacology (2021) IF: 5.811
    Identification of the Antithrombotic Mechanism of Leonurine in Adrenalin Hydrochloride-Induced Thrombosis in Zebrafish via Regulating Oxidative Stress and Coagulation Cascade.

    DOI: 10.3389/fphar.2021.742954
  8. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2022) IF: 4.101
    Epithelial‑derived exosomes promote M2 macrophage polarization via Notch2/SOCS1 during mechanical ventilation

    DOI: 10.3892/ijmm.2022.5152

    PMID: 35616134

    		 Sample: MLE-12 cell
  9. Journal of Cardiovascular Translational Research (2022) IF: 4.132
    CD137 Signal Mediates Cardiac Ischemia–Reperfusion Injury by Regulating the Necrosis of Cardiomyocytes

    DOI: 10.1007/s12265-022-10240-1
    Sample: serum,HL-1 cell
  10. EUROPEAN JOURNAL OF PHARMACOLOGY (2021) IF: 4.432
    Glycyrrhizic acid ameliorates myocardial ischemia-reperfusion injury in rats through inhibiting endoplasmic reticulum stress

    DOI: 10.1016/j.ejphar.2021.174353

    PMID: 34274339

    		 Sample: serum
  11. CELL BIOLOGY INTERNATIONAL (2022) IF: 4.473
    Overexpression of TRIM32 promotes pancreatic β-cell autophagic cell death through Akt/mTOR pathway under high glucose conditions

    DOI: 10.1002/cbin.11897
    Sample: INS-1 cell
  12. AMERICAN JOURNAL OF CHINESE MEDICINE (2021) IF: 4.667
    Identification of Chemical Compounds from Artemisia gmelinii using UPLC-QTOF-MS/MS and their Regulatory Effects on Immune Responses in DSS-Induced Colitis Mice

    DOI: 10.1142/S0192415X21500452

    PMID: 33827384

    		 Sample: serum
  13. Vaccines (2022) IF: 4.961
    Development of a Bispecific Nanobody Targeting CD20 on B-Cell Lymphoma Cells and CD3 on T Cells

    DOI: 10.3390/vaccines10081335

    PMID: 36016223

    		 Sample: PBMC
  14. Scientific Reports (2022) IF: 4.996
    Gum Arabic nanoformulation rescues neuronal lesions in bromobenzene-challenged rats by its antioxidant, anti-apoptotic and cytoprotective potentials

    DOI: 10.1038/s41598-022-24556-0
    Sample: Brain
  15. NEUROSCIENCE LETTERS (2021) IF: 3.046
    Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence

    DOI: 10.1016/j.neulet.2021.136385

    PMID: 34871743

    		 Sample: serum
  16. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2023) IF: 3.195
    Glycoside combinations of Buyang Huanwu decoction ameliorate atherosclerosis via STAT3, HIF-1, and VEGF

    DOI: 10.1007/s00210-023-02389-6
    Sample: rat thoracic aortic endothelial cells (RTAEC)
  17. REPRODUCTION (2020) IF: 3.206
    Orlistat reverses intratesticular lactate transport decline and infertility in male obese rats

    DOI: 10.1530/REP-20-0381

    PMID: 33112813

    		 Sample: testis
  18. JOURNAL OF GENE MEDICINE (2020) IF: 3.258
    miR‐129‐5p attenuates hypoxia‐induced apoptosis in rat H9c2 cardiomyocytes by activating autophagy

    DOI: 10.1002/jgm.3200
    Sample: H9c2 cell
  19. RSC Advances (2021) IF: 3.361
    Immunomodulatory and antimicrobial non-mulberry Antheraea mylitta silk fibroin accelerates in vitro fibroblast repair and regeneration by protecting oxidative stress

    DOI: 10.1039/D0RA08538C
    Sample: Cell sample
  20. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018) IF: 3.575
    Celastrol pretreatment attenuates rat myocardial ischemia/ reperfusion injury by inhibiting high mobility group box 1 protein expression via the PI3K/Akt pathway

    DOI: 10.1016/j.bbrc.2018.02.121
    Sample: serum
  21. METABOLIC BRAIN DISEASE (2022) IF: 3.584
    Hepatoprotective and neuroprotective effect of taxifolin on hepatic encephalopathy in rats

    DOI: 10.1007/s11011-022-00952-3

    PMID: 35298730

    		 Sample: serum
  22. LIFE SCIENCES (2020) IF: 3.647
    Naringin ameliorates type 2 diabetes mellitus-induced steatohepatitis by inhibiting RAGE/NF-κB mediated mitochondrial apoptosis

    DOI: 10.1016/j.lfs.2020.118118
    Sample: serum
  23. MICROVASCULAR RESEARCH (2023) IF: 3.75
    Co-administration of metformin and/or glibenclamide with losartan reverse NG-nitro-l-arginine-methyl ester-streptozotocin-induced hypertensive diabetes and haemodynamic sequelae in rats

    DOI: 10.1016/j.mvr.2023.104497
    Sample: Serum
  24. JOURNAL OF BIOMATERIALS APPLICATIONS (2021) IF: 2.646
    Multi-layered in vitro 3D-bone model via combination of osteogenic cell sheets with electrospun membrane interlayer:

    DOI: 10.1177/08853282211027889
    Sample: MC3T3-E1 cells
  25. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
    San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

    DOI: 10.1155/2022/7832540
    Sample: hepatic tissue,serum
  26. Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022) IF: 2.693
    Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains

    DOI: 10.1002/jez.2603
    Sample: brain
  27. ACS Omega (2020) IF: 2.87
    Extract from Rostellularia procumbens (L.) Nees Inhibits Thrombosis and Platelet Aggregation by Regulating Integrin β3 and MAPK Pathways

    DOI: 10.1021/acsomega.0c05227
    Sample: serum
  28. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2019) IF: 2.928
    Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway

    DOI: 10.3892/ijmm.2019.4123
    Sample: Serum
  29. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2019) IF: 2.965
    Hesperidin regresses cardiac hypertrophy by virtue of PPAR‐γ agonistic, anti‐inflammatory, antiapoptotic, and antioxidant properties

    DOI: 10.1002/jbt.22283
    Sample: Serum
  30. TROPICAL ANIMAL HEALTH AND PRODUCTION (2020) IF: 1.333
    Thermal stress consequences on growth performance, immunological response, antioxidant status, and profitability of finishing broilers: transcriptomic profile change of stress-related genes

    DOI: 10.1007/s11250-020-02405-4

    PMID: 32978744

    		 Sample: Serum
  31. Molecular Medicine Reports (2016) IF: 1.559
    Protective effects of ghrelin against oxidative stress, inducible nitric oxide synthase and inflammation in a mouse model of myocardial ischemia/reperfusion injury via the HMGB1 and TLR4/NF-κB pathway

    DOI: 10.3892/mmr.2016.5535
    Sample: serum
  32. TOXICOLOGY AND INDUSTRIAL HEALTH (2020) IF: 1.708
    Elevated, sustained, and yet reversible biotoxicity effects of lead on cessation of exposure: Melatonin is a potent therapeutic option:

    DOI: 10.1177/0748233720937199
    Sample: Plasma
  33. Heliyon (2021)
    Treatment with acetate during late pregnancy protects dams against testosterone-induced renal dysfunction

    DOI: 10.1016/j.heliyon.2021.e05920
    Sample: Renal
  34. Toxicology Reports (2019)
    Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

    DOI: 10.1016/j.toxrep.2019.06.009
    Sample: plasma
  35. Advances in Traditional Medicine (2020)
    Anticandidal efficacy of Brassica juncea seeds extract: characterization, in vitro and in vivo studies

    DOI: 10.1007/s13596-020-00440-y
    Sample: serum
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Reviews/Q&A

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  • Reviews
  • Q&A

Verified Customer

p****pSubmitted [ Jun 06 2019 ]

  • Application:Biochemical
  • Description:I used this kit to detect the content of lactate dehydrogenase in human serum and got good results. This kit is easy to operate, short detection time, with sample control to reduce interference, is a great design.
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Q

N************RSubmitted [ Aug 05 2023 ]

Asked: Is this kit appropriate for studying with plants (lichens)?

Reply

A

adminSubmitted [ Aug 05 2023 ]

Answered: We'll reply to you by email. Pls pay attention to the mailbox

Q

M*****iSubmitted [ Oct 09 2022 ]

Asked: Why doesn\'t my standard curve go through the origin？

Reply

A

adminSubmitted [ Oct 09 2022 ]

Answered: Please use the absolute OD value for plotting.

Q

M*****iSubmitted [ Aug 25 2022 ]

Asked: What is the minimum volume of sample? I\'m detecting mouse serum.

Reply

A

adminSubmitted [ Aug 25 2022 ]

Answered: A minimum volume of 40 uL is required.

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