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Lactate Dehydrogenase (LDH) Activity Assay Kit (WST-8 Method)

Cat.No.:E-BC-K766-M
Detection instrument: Microplate reader (450 nm)

Detection method: Colorimetric method

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Detection principle

Lactate dehydrogenase (LDH) is an oxidoreductase. LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back. LDH is composed of four subunits (tetramer). The two most common subunits are the LDH-M and LDH-H protein. LDH is released into the blood by cells after tissue damage or erythrocyte hemolysis. Extracellular LDH activity is used to detect cell damage or cell death

Performance characteristics

Synonyms	LDH
Sample type	Serum, plasma, animal tissue, hydrothorax, cells, cell culture supernatant
Sensitivity	0.11 U/L
Detection range	0.11-39.9 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	30 min
Precision	Average inter-assay CV: 2.300%Average intra-assay CV: 2.300%
Other instruments required	Microplate, Micropipettor, Vortex mixer, Water bath
Storage	-20℃
Valid period	12 months

Images

LDH activity in serum was significantly increased by NB. (**P<0.01, ***P<0.001)

Q Li et al investigate the hypoxia-induced neurogenic bladder fibrosis. Lactate dehydrogenase (LDH) activity of mouse and human serum was determined using LDH activity assay kit (E-BC-K766-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.11-39.9 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	10-20
Dog serum	10-20
Mouse serum	50-100
Cynomolgus monkey serum	10-20
10% Rat spleen tissue homogenate	150-250
10% Rat liver tissue homogenate	250-350
10% Rat kidney tissue homogenate	250-350
10% Rat lung tissue homogenate	250-350

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Cells (2022) IF: 7.666
Hypoxia-Induced HIF-1α Expression Promotes Neurogenic Bladder Fibrosis via EMT and Pyroptosis

DOI: 10.3390/cells11233836

Sample: serum
FOOD AND CHEMICAL TOXICOLOGY (2022) IF: 6.025
Procyanidins inhibit zearalenone-induced apoptosis and oxidative stress of porcine testis cells through activation of Nrf2 signaling pathway

DOI: 10.1016/j.fct.2022.113061

PMID: 35489465
Sample: pig testicular cell (ST cell)
Food Bioscience (2022) IF: 5.318
Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

DOI: 10.1016/j.fbio.2022.102079

Sample: livers
Foods (2022) IF: 5.561
Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

DOI: 10.3390/foods11193048

PMID: 36230124
Sample: serum
Journal of Cardiovascular Translational Research (2022) IF: 4.132
CD137 Signal Mediates Cardiac Ischemia–Reperfusion Injury by Regulating the Necrosis of Cardiomyocytes

DOI: 10.1007/s12265-022-10240-1

Sample: serum,HL-1 cell
CELL BIOLOGY INTERNATIONAL (2022) IF: 4.473
Overexpression of TRIM32 promotes pancreatic β-cell autophagic cell death through Akt/mTOR pathway under high glucose conditions

DOI: 10.1002/cbin.11897

Sample: INS-1 cell
AMERICAN JOURNAL OF CHINESE MEDICINE (2021) IF: 4.667
Identification of Chemical Compounds from Artemisia gmelinii using UPLC-QTOF-MS/MS and their Regulatory Effects on Immune Responses in DSS-Induced Colitis Mice

DOI: 10.1142/S0192415X21500452

PMID: 33827384
Sample: serum
Vaccines (2022) IF: 4.961
Development of a Bispecific Nanobody Targeting CD20 on B-Cell Lymphoma Cells and CD3 on T Cells

DOI: 10.3390/vaccines10081335

PMID: 36016223
Sample: PBMC
NEUROSCIENCE LETTERS (2021) IF: 3.046
Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence

DOI: 10.1016/j.neulet.2021.136385

PMID: 34871743
Sample: serum
REPRODUCTION (2020) IF: 3.206
Orlistat reverses intratesticular lactate transport decline and infertility in male obese rats

DOI: 10.1530/REP-20-0381

PMID: 33112813
Sample: testis
JOURNAL OF GENE MEDICINE (2020) IF: 3.258
miR‐129‐5p attenuates hypoxia‐induced apoptosis in rat H9c2 cardiomyocytes by activating autophagy

DOI: 10.1002/jgm.3200

Sample: H9c2 cell
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018) IF: 3.575
Celastrol pretreatment attenuates rat myocardial ischemia/ reperfusion injury by inhibiting high mobility group box 1 protein expression via the PI3K/Akt pathway

DOI: 10.1016/j.bbrc.2018.02.121

Sample: serum
LIFE SCIENCES (2020) IF: 3.647
Naringin ameliorates type 2 diabetes mellitus-induced steatohepatitis by inhibiting RAGE/NF-κB mediated mitochondrial apoptosis

DOI: 10.1016/j.lfs.2020.118118

Sample: serum
MICROVASCULAR RESEARCH (2023) IF: 3.75
Co-administration of metformin and/or glibenclamide with losartan reverse NG-nitro-l-arginine-methyl ester-streptozotocin-induced hypertensive diabetes and haemodynamic sequelae in rats

DOI: 10.1016/j.mvr.2023.104497

Sample: Serum
JOURNAL OF BIOMATERIALS APPLICATIONS (2021) IF: 2.646
Multi-layered in vitro 3D-bone model via combination of osteogenic cell sheets with electrospun membrane interlayer:

DOI: 10.1177/08853282211027889

Sample: MC3T3-E1 cells
Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

DOI: 10.1155/2022/7832540

Sample: hepatic tissue,serum
Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022) IF: 2.693
Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains

DOI: 10.1002/jez.2603

Sample: brain
Molecular Medicine Reports (2016) IF: 1.559
Protective effects of ghrelin against oxidative stress, inducible nitric oxide synthase and inflammation in a mouse model of myocardial ischemia/reperfusion injury via the HMGB1 and TLR4/NF-κB pathway

DOI: 10.3892/mmr.2016.5535

Sample: serum
Toxicology Reports (2019)
Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

DOI: 10.1016/j.toxrep.2019.06.009

Sample: plasma

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