Lactulose Fluorometric Assay Kit (E-BC-F049)
For research use only.
| Detection Principle |
Lactulose (4-o-β-galactopyranosyl-D-fructo-furanose), also known as galactoside fructose, 4-β-D-galactosid-D-fructose and lactulose, contains one molecule of galactose and one molecule of fructose. Lactulose is a kind of synthetic disaccharide formed by base isomerization of lactose catalyzed by free amino group of casein during milk heat treatment. Lactulose can produce a specific product under the action of enzyme, which reacts with the chromogenic agent to produce a fluorescence product. The excitation wavelength is 530 nm, and the emission wavelength is 590 nm. |
| Sample Type | Dairy product,Feces |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=530 nm/590 nm) |
| Research Area | Metabolic Diseases |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 4.78 μmol/L |
| Detection Range | 4.78-500 μmol/L |
| Precision | inter-assay CV: 7.4-9.7% | intra-assay CV: 2.6-3.8% |
| Sample Volume | 50μL |
| Assay Time | 2 h 30 min |
Note: Data for this module come from published literature that cites this product, displaying other products used together in the same research paper.
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Fresh milk 1 | 5-10 |
| Fresh milk 2 | 5-10 |
| Pure milk | 5-10 |
| Infant feces | 2-6 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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