Purified Anti-Mouse CD16/32 Antibody[2.4G2], Functional Grade (E-AB-F09970)
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For research use only.
| Alternate Names | CD16a/b, CD32, CD32A/B, FCG2A, FCGR2A/BFCGR3, FCGR3A/B, Fc fragment of IgG low affinity IIIa/b receptor, Fc fragment of IgG low affinity IIIb receptor, Fc fragment of IgG low affinity IIa/b receptor, Fc gamma RIIa/b , Fc gamma receptor III A/B, FcGR |
| Clone | |
| Leadtime | Order now, ship in 3 days |
| Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells), Block 0.5-1 μg(0.5×106-1×106 cells) in 100 μL |
| Background | CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant Mouse CD16/32 protein |
| Abbre | CD16/32 |
| Swissprot | |
| Host | Rat |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Rat IgG2b, κ |
| Isotype Control | Purified Rat IgG2b, κ Isotype Control[LTF-2], Functional Grade |
| Applications |
FCM;Block
|
| Research Areas | Immunology;Innate Immunity |
| Concentration |
≥ 1 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Application References | Giulia Pasqual, et al. Nature. 2018 Jan 25;553(7689):496-500. |
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