LTB4(Leukotriene B4) ELISA Kit (E-EL-0061)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal LTB4. During the reaction, Universal LTB4 in the sample or standard competes with a fixed amount of Universal LTB4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal LTB4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal LTB4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Leukotriene B4 [LTB4; (5S,12R)-5,12-dihydroxy-(Z,E,E,Z)-6,8,10,14-eicosatetraenoic acid] is a potent chemotactic agentgenerated enzymatically in leukocytes from arachidonic acid viathe 5-lipoxygenase pathway. Previous studies demonstrated thatLTB4 is generated in several cell types including polymorphonuclear leukocytes (PMNs), macrophages, and monocytes . Asa potent chemotactic agent, LTB4 is regarded as an importantmediator in several pathological processes such as inflammatoryand allergic responses. To understand the role of LTB4 in thesepathological processes, an accurate and reliable analytical methodto determine the concentration of LTB4 in biological fluids isrequired. However, due to the very low concentration of endogenous LTB4 and the potential interference from other endogenouscompounds in the biological fluids, it is challenging to develop asufficiently sensitive and selective method.