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Magnesium (Mg) Colorimetric Assay Kit

Cat.No.:E-BC-K162-S
Detection instrument: Spectrophotometer(540 nm)

Detection method: Colorimetric method

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Detection principle

The magnesium in the serum reacts with the complexometric indicator (Calmagite) to form the Calmagite-Mg compound. The absorbance of this compound at 540 nm is proportional to the concentration of magnesium in the sample. The concentration of magnesium can be calculated by measuring the OD value at 540 nm.

Performance characteristics

Synonyms	Mg
Sample type	Serum,plasma
Sensitivity	0.12 mmol/L
Detection range	0.12-2.50 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	50 min
Precision	Average inter-assay CV: 9.900%Average intra-assay CV: 4.800%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

The levels of Mg concentration was significantly changed comparing to the diseased control. (**P<0.01, ***P<0.001)

A Raja et al investigate the anti-osteoporotic effect of Matricaria chamomilla L. Flower. Magnesium (Mg) level in rat serum was determined using Mg colorimetric assay kit (E-BC-K162-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.12-2.50 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Rat serum	1
Mouse serum	1
Porcine serum	1
Chicken serum	1
Dog serum	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Antioxidants (2022) IF: 7.675
Prophylactic Anti-Osteoporotic Effect of Matricaria chamomilla L. Flower Using Steroid-Induced Osteoporosis in Rat Model and Molecular Modelling Approaches

DOI: 10.3390/antiox11071316

PMID: 35883807
Sample: Serum

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