Magnesium (Mg) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
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    >
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K162-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $200
      Can detect 96 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(540 nm)

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      The magnesium in the serum reacts with the complexometric indicator (Calmagite) to form the Calmagite-Mg compound. The absorbance of this compound at 540 nm is proportional to the concentration of magnesium in the sample. The concentration of magnesium can be calculated by measuring the OD value at 540 nm.

      Performance characteristics

      Synonyms Mg
      Sample type serum,plasma
      Sensitivity 0.12 mmol/L
      Detection range 0.12-2.50 mmol/L
      Precision Average inter-assay CV: 9.9%Average intra-assay CV: 4.8%
      Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months

      Dilution of sample

      It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.12-2.50 mmol/L).

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

      Human serum

      1

      Rat serum

      1

      Mouse serum

      1

      Porcine serum

      1

      Chicken serum

      1

      Dog serum

      1

      Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

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