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MAP2K2 Monoclonal Antibody

Cat:E-AB-22162 Citations (1)
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Mouse
  • Reactivity: Human;Mouse;Rat
  • Applications: WB
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Product Details
Verified Samples Verified Samples in WB:Hela,3T3,Rat brain
Dilution

WB 1:500-1:2000, IHC 1:100-1:300, IP 1:200-1:500, ELISA 1:10000

Western Blot Operation Guide
Clonality Monoclonal
Immunogen Synthesized peptide derived from human MEK-2 around the non-phosphorylation site of T394.
Abbre MEK2
Synonyms Cardiofaciocutaneous syndrome;CFC syndrome;CFC4;Dual specificity mitogen activated protein kinase kinase 2;Dual specificity mitogen-activated protein kinase kinase 2;ERK activator kinase 2;FLJ26075;MAP kinase kinase 2;map2k2;MAPK / ERK kinase 2;MAPK/ERK kinase 2;MAPKK 2;MAPKK2;MEK 2;MEK2;Microtubule associated protein kinase kinase 2;Mitogen activated protein kinase kinase 2;Mitogen activated protein kinase kinase 2 p45;MKK 2;MKK2;MP2K2;OTTHUMP00000165826;OTTHUMP00000165827;PRKMK 2;PRKMK2
Swissprot
Observed MW 45kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoskeleton,microtubule,Cytosol,Endoplasmic reticulum,endoplasmic reticulum,Endosome,early endosome,late endosome,Extracellular region or secreted,extracellular region,Golgi apparatus,Golgi apparatus,Mitochondrion,Nucleus,Peroxisome,peroxisomal membrane,Plasma Membrane,cytoplasmic side of Plasma Membrane,Other locations:cell-cell junction,focal adhesion,perinuclear region of cytoplasm
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Protein A purification
Research Areas Cancer; Signal Transduction
Clone No. 2C3
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.