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MDA(Malondialdehyde) ELISA Kit

  • Cat.No.:E-EL-0060

  • Reactivity: Universal

To Purchase E-EL-0060

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 31.25-2000 ng/mL
Sensitivity 18.75 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes MDA in samples. No significant cross-reactivity or interference between MDA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of MDA concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MDA. During the reaction, MDA in the sample or standard competes with a fixed amount of MDA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MDA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MDA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level MDA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level MDA were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 109.60 308.70 848.80 111.50 321.00 892.50
Standard deviation 6.10 17.30 37.30 7.00 17.00 46.40
CV (%) 5.57 5.60 4.39 6.28 5.30 5.20


The recovery of MDA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-103 97
EDTA plasma(n=8) 94-107 99
Cell culture media(n=8) 93-110 100

Target Information

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


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  1. Redox Biology (2020) IF: 9.9860
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    Sample: Tissue homogenate
  2. Cancers (2020) IF: 6.126
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    DOI: 10.3390/cancers12061526

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    Sample: Serum
  3. Cancer Cell International (2022) IF: 5.722
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    DOI: 10.1186/s12935-022-02543-9

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    Sample: Serum
  4. RNA biology (2019) IF: 5.35
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    DOI: 10.1080/15476286.2019.1657352

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    Sample: Serum,Cell culture medium
  5. development (2019) IF: 5.413
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    Sample: Serum
  6. Biomedicine & Pharmacotherapy (2020) IF: 4.545
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    DOI: 10.1016/j.biopha.2020.110304

    PMID: 32497865

    Sample: Serum
  7. Nutrients (2020) IF: 4.546
    The Effects of Tocotrienol-Rich Vitamin E (Tocovid) on Diabetic Neuropathy: A Phase II Randomized Controlled Trial

    DOI: 10.3390/nu12051522

    PMID: 32456230

    Sample: Serum
  8. Journal of Cellular and Molecular Medicine (2018) IF: 4.499
    Modulation of the oxidative plasmatic state in gastroesophageal reflux disease with the addition of rich water molecular hydrogen: A new biological vision.

    DOI: 10.1111/jcmm.13569

    PMID: 29512923

  9. Science of The Total Environment (2017) IF: 4.9
    Long-Term Exposure To High Air Pollution Induces Cumulative DNA Damages In Traffic Policemen.

    DOI: 10.1016/j.scitotenv.2017.03.179

    PMID: 28346906

    Sample: Serum
  10. Environmental Toxicology and Pharmacology (2020) IF: 3.292
    Nicotine-mediated upregulation of microRNA-141 expression determines adipokine-intervened insulin resistance

    DOI: 10.1016/j.etap.2020.103506

    PMID: 33002592

    Sample: Serum
  11. Inflammopharmacology (2020) IF: 3.238
    Perindopril ameliorates experimental Alzheimer's disease progression: role of amyloid β degradation, central estrogen receptor and hyperlipidemic-lipid raft signaling

    DOI: 10.1007/s10787-020-00724-4

    PMID: 32488543

    Sample: Serum,Tissue homogenate
  12. Toxicology and Applied Pharmacology (2020) IF: 3.347
    Low molecular polypeptide from oyster hydrolysate recovers photoaging in SKH-1 hairless mice

    DOI: 10.1016/j.taap.2019.114844

    PMID: 31785243

    Sample: Serum
  13. International Journal of Neuropsychopharmacology (2018) IF: 3.981
    Emodin rescued hyperhomocysteinemia-induced dementia and Alzheimer's disease-like features in rats

    DOI: 10.1093/ijnp/pyy090

    PMID: 30407508

    Sample: Plasma,Tissue homogenate
  14. Food Research International (2018) IF: 3.52
    The effect of kefir produced from natural kefir grains on the intestinal microbial populations and antioxidant capacities of Balb/c mice

    DOI: 10.1016/j.foodres.2018.10.080

    PMID: 30599959

    Sample: Serum
  15. Environmental Toxicology and Pharmacology (2020) IF: 3.292
    Chronic exposure of bisphenol A impairs carbohydrate and lipid metabolism by altering corresponding enzymatic and metabolic pathways

    DOI: 10.1016/j.etap.2020.103387

    PMID: 32339907

    Sample: Serum
  16. Scientific Reports (2020) IF: 3.998
    Reproductive effects of subchronic exposure to acetamiprid in male rats

    DOI: 10.1038/s41598-020-65887-0

    PMID: 32488017

    Sample: Plasma,Tissue homogenate
    2,3,5,4'-tetrahydroxystilbence-2-O-β-D-glucoside attenuates hepatic steatosis via IKKβ/NF-κB and Keap1-Nrf2 pathways in larval zebrafish

    DOI: 10.1016/j.biopha.2020.110138

    Sample: Tissue homogenate
  18. International Journal of Neuropsychopharmacology IF: 3.98
    Emodin rescued hyperhomocysteinemia-induced dementia and Alzheimer's disease-like features in rats

    DOI: 10.1093/ijnp/pyy090

    PMID: 30407508

    Sample: Serum, Tissue homogenate
  19. Anatolian Journal of Cardiology (2018) IF: 3.767
    Protective Effects of Curcumin and Beta-carotene on Cisplatin-induced Cardiotoxicity: An Experimental Rat Model.

    DOI: 10.14744/AnatolJCardiol.2018.53059

    PMID: 29521316

  20. Experimental Gerontology (2017) IF: 3.34
    Dried Plum And Chokeberry Ameliorate D-Galactose-Induced Aging In Mice By Regulation Of Pl3K/Akt-Mediated Nrf2 And Nf-kB Pathways.

    DOI: 10.1016/j.exger.2017.05.004

    PMID: 28502776

    Sample: Serum
  21. Iubmb Life (2014) IF: 3.141
    2, 3, 5, 4' -Tetrahydroxystilbene-2-O-β-D-Glucoside Protects Human Umbilical Vein Endothelial Cells Against Lysophosphatidylcholine-Induced Apoptosis By Upregulating Superoxide Dismutase And Glutathione Peroxidase.

    DOI: 10.1002/iub.1321

    PMID: 25382724

    Sample: Cell lysate
  22. Animals (2020) IF: 2.323
    Obesity-Induced Heart Rate Variability Impairment and Decreased Systolic Function in Obese Male Dogs

    DOI: 10.3390/ani10081383

    PMID: 32785083

    Sample: Plasma
  23. Saudi Journal of Biological Sciences (2020) IF: 2.802
    Curcumin-loaded cockle shell-derived calcium carbonate nanoparticles: A novel strategy for the treatment of lead-induced hepato-renal toxicity in rats

    DOI: 10.1016/j.sjbs.2020.03.009

    PMID: 32489292

    Sample: Serum
  24. Saudi Pharmaceutical Journal (2020) IF: 2.879
    Possible combined effect of perindopril and Azilsartan in an experimental model of dementia in rats

    DOI: 10.1016/j.jsps.2020.03.009

    PMID: 32435138

    Sample: Tissue homogenate
  25. Therapeutic advances in endocrinology and metabolism (2019) IF: 2.173
    Tocotrienol-rich vitamin E improves diabetic nephropathy and persists 6-9 months after washout: a phase IIa randomized controlled trial

    DOI: 10.1177/2042018819895462

    PMID: 31903178

    Sample: Serum
  26. Molecular Medicine Reports (2017) IF: 2.1
    AMPK activation restores ischemic post-conditioning cardioprotection in STZ-induced type 1 diabetic rats: Role of autophagy

    DOI: 10.3892/mmr.2017.7033

    PMID: 28765969

    Sample: Serum
  27. Molecular medicine reports (2016) IF: 2.1
    Nobiletin ameliorates isoflurane-induced cognitive impairment via antioxidant, anti-inflammatory and anti-apoptotic effects in aging rats

    DOI: 10.3892/mmr.2016.5919

    PMID: 27840933

    Sample: Serum
  28. Nutrition Research (2018) IF: 2.707
    Additive effect of walnut and chokeberry on regulation of antioxidant enzyme gene expression and attenuation of lipid peroxidation in d-galactose-induced aging-mouse model

    DOI: 10.1016/j.nutres.2018.09.011

    PMID: 30446253

    Sample: Serum
  29. Molecular vision (2019) IF: 2.219
    Polyphenol-enriched fraction of Vaccinium uliginosum L. protects selenite-induced cataract formation in the lens of Sprague-Dawley rat pups

    PMID: 30820147

    Sample: Tissue homogenate
  30. ACS omega (2020) IF: 2.87
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    DOI: 10.1021/acsomega.0c01670

    PMID: 32803042

    Sample: Serum
  31. Biomedical and Environmental Sciences (2018) IF: 2.518
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    DOI: 10.3967/bes2018.101

    PMID: 30423277

    Sample: Plasma,Tissue homogenate
  32. BMC pulmonary medicine (2018) IF: 2.721
    NOX4 expression and distal arteriolar remodeling correlate with pulmonary hypertension in COPD.

    DOI: 10.1186/s12890-018-0680-y

    PMID: 29986678

    Sample: Serum
  33. Neurochemical Research (2017) IF: 2.581
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    DOI: 10.1007/s11064-017-2389-3

    PMID: 28852940

    Sample: Tissue homogenate
  34. Molecular Vision (2017) IF: 2.174
    Pinus Densiflora Bark Extract Prevents Selenite-induced Cataract Formation In The Lens Of Sprague Dawley Rat Pups.

    PMID: 28943754

    Sample: Tissue homogenate
  35. Clinical and Experimental Pharmacology and Physiology (2016) IF: 2.01
    A Novel Translocator Protein 18 kDa Ligand, ZBD-2, Exerts Neuroprotective Effects Against Acute Spinal Cord Injury.

    DOI: 10.1111/1440-1681.12606

    PMID: 27292096

    Sample: Serum
  36. The Korean Journal of Physiology & Pharmacology: Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology (2020) IF: 1.805
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    DOI: 10.4196/kjpp.2020.24.5.385

    PMID: 32830145

    Sample: Tissue homogenate
  37. Anticancer Research (2020) IF: 1.994
    The Induction of Antioxidant Catalase Enzyme With Decrease of Plasma Malonidialdehyde: An Important Reactive Oxidative Species Inhibiting Mechanism

    DOI: 10.21873/anticanres.14584

    PMID: 32988895

    Sample: Plasma
  38. European Journal of Medical Research (2020) IF: 1.826
    Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function

    DOI: 10.1186/s40001-020-00431-4

    PMID: 32738910

    Sample: Tissue homogenate
  39. Anticancer Research (2020) IF: 1.994
    The Rectus Sheath Block (RSB) Analgesia Following Laparotomy Could Affect Malonidialdehyde (MDA) Concentrations in Benign Disease and Cancer

    DOI: 10.21873/anticanres.13947

    PMID: 31892574

    Sample: Plasma
  40. Annals of Surgical Treatment and Research (2019) IF: 1.367
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    DOI: 10.4174/astr.2019.97.6.271

    PMID: 31824881

    Sample: Serum,Tissue homogenate
  41. Current Eye Research (2020) IF: 1.754
    Expression of α-Klotho Is Downregulated and Associated with Oxidative Stress in the Lens in Streptozotocin-induced Diabetic Rats

    DOI: 10.1080/02713683.2020.1805768

    PMID: 32744464

    Sample: Tissue homogenate
  42. Journal of Zhejiang University-Science B (2016) IF: 1.676
    Myricetin Protects Against Diet-Induced Obesity And Ameliorates Oxidative Stress In C57BL/6 Mice.

    DOI: 10.1631/jzus.B1600074

    PMID: 27256677

    Sample: Serum
  43. Journal of Investigative Surgery (2017) IF: 1.532
    Ozone Partially Decreases Axonal And Myelin Damage In An Experimental Sciatic Nerve Injury Model.

    DOI: 10.1080/08941939.2017.1369606

    PMID: 28925753

    Sample: Serum
  44. Molecular Medicine Reports (2015) IF: 1.692
    Asiaticoside Attenuates The Effects Of Spinal Cord Injury Through Antioxidant And AntiInflammatory Effects, And Inhibition Of The p38MAPK Mechanism.

    DOI: 10.3892/mmr.2015.4425

    PMID: 26458544

    Sample: Serum
  45. Asian J Pharm Clin Res (2019)

    DOI: 10.22159/ajpcr.2019.v12i2.30815

    Sample: Serum
  46. Int J Recent Sci Res (2015)
    Interleukin-17: a cardiac biomarker in estimation of cardioprotective effects of tacrolimus in doxorubicin-induced cardiotoxicity: animal model study
    Sample: Serum
  47. Indian Journal of Forensic Medicine & Toxicology (2019)
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    DOI: 10.5958/0973-9130.2019.00466.3

    Sample: Serum,Saliva
  48. Al-Qadisiyah Journal Of Pure Science (2020)
    Roles of Superoxide dismutase(SOD) , Malondialdehyde (MDA), 8-iso-prostaglandinF2α ( 8-iso-PGF2α)as oxidative stress in development and progression of Brest cancer in Iraqi females patients

    DOI: 10.29350/jops.2020.25.1.1053

    Sample: Plasma
  49. International Journal of Rheumatology (2020)
    Correlation between C-reactive Protein with Malondialdehyde in Systemic Lupus Erythematosus Patients

    DOI: 10.1155/2020/8078412

    PMID: 32695177

    Sample: Serum
  50. Preprint (2020)
    Electroacupuncture preconditioning alleviated myocardial injury via regulating mitochondrial function

    DOI: 10.21203/

    Sample: Tissue homogenate
  51. Proff. Sameerah Ahmed (2020)
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    DOI: 10.2139/ssrn.3559385

    Sample: Plasma
  52. Mustansiriya Medical Journal (2018)
    Evaluation of the effects of TAK-242 and GIT-27 on methotrexate-induced liver injury

    DOI: 10.4103/MJ.MJ_15_18

    Sample: Serum
  53. Universitas Airlangga (2018)
    Sample: Serum
  54. Journal of Pharmaceutical Sciences and Research (2018)
    Protective effect of montelukast against acute kidney injury in rats induced by diclofenac
    Sample: Serum
  55. Indian Journal of Forensic Medicine & Toxicology (2020)
    Reduced Glutathione, Lipid Peroxidation and Malondialdehyde Status in Women with Mild and Severe Preeclampsia for Babylon Governorate

    DOI: 10.37506/ijfmt.v14i1.144

    Sample: Serum
  56. Drug Invention Today (2019)
    Effects of Vitamin D3 on methotrexate-induced jejunum damage in rats by the utilization of immunohistochemical study

    DOI: 10.31351/vol29iss1pp260-267

    Sample: Tissue homogenate
  57. Nigerian Postgraduate Medical Journal (2018) IF: /
    Assessment of seminal biomarker of lipid peroxidation among male partners of infertile couples at the university of ilorin teaching hospital, Nigeria.

    DOI: 10.4103/npmj.npmj_51_18

    PMID: 30027920

    Sample: Sperm
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