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MDA(Malondialdehyde) ELISA Kit

Citations(151)

  • Cat.No.:E-EL-0060

  • Reactivity: Universal
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Manual MSDS

To Purchase E-EL-0060

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 31.25-2000 ng/mL
Sensitivity 18.75 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes MDA in samples. No significant cross-reactivity or interference between MDA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of MDA concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MDA. During the reaction, MDA in the sample or standard competes with a fixed amount of MDA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MDA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MDA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level MDA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level MDA were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 109.60 308.70 848.80 111.50 321.00 892.50
Standard deviation 6.10 17.30 37.30 7.00 17.00 46.40
CV (%) 5.57 5.60 4.39 6.28 5.30 5.20

Recovery

The recovery of MDA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-103 97
EDTA plasma(n=8) 94-107 99
Cell culture media(n=8) 93-110 100

Target Information

Research AreaNeuroscience, Signal transduction, Metabolism

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
elisa assay procedure 2

2. Aspirate and wash the plate for 3 times
elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 5

5. Add 50μL Stop Solution
elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. BIOMATERIALS (2022) IF: 15.304
    Endoplasmic reticulum-targeted inhibition of CYP2E1 with vitamin E nanoemulsions alleviates hepatocyte oxidative stress and reverses alcoholic liver disease

    DOI: 10.1016/j.biomaterials.2022.121720

    PMID: 35961822

    		 Sample: primary Kupffer cell
  2. Advanced Fiber Materials (2023) IF: 12.958
    Double-Layer Nanofibrous Sponge Tube via Electrospun Fiber and Yarn for Promoting Urethral Regeneration

    DOI: 10.1007/s42765-022-00252-6
    Sample: Human foreskin fibroblasts (HFFs),HSF cell
  3. Journal of Advanced Research (2022) IF: 10.479
    Cadmium accumulation regulated by a rice heavy-metal importer is harmful for host plant and leaf bacteria

    DOI: 10.1016/j.jare.2022.05.010

    PMID: 35640876

    		 Sample: leaf
  4. Redox Biology (2020) IF: 9.9860
    Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor

    DOI: 10.1016/j.redox.2020.101514

    PMID: 32234613

    		 Sample: Tissue homogenate
  5. FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.1010
    Inhibition of ferroptosis attenuates oligospermia in male Nrf2 knockout mice

    DOI: 10.1016/j.freeradbiomed.2022.10.314

    PMID: 36309297

    		 Sample: seminal plasma
  6. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    ZNF354C Mediated by DNMT1 Ameliorates Lung Ischemia-Reperfusion Oxidative Stress Injury by Reducing TFPI Promoter Methylation to Upregulate TFPI

    DOI: 10.1155/2022/7288729

    PMID: 35915612

    		 Sample: bronchoalveolar lavage fluid (BALF),HUVECs
  7. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Downregulation of SIRT3 Aggravates Lung Ischemia Reperfusion Injury by Increasing Mitochondrial Fission and Oxidative Stress through HIF-1α-Dependent Mechanisms

    DOI: 10.1155/2022/9041914
    Sample: lung tissue
  8. FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 7.376
    Nrf2 and NF-κB/NLRP3 inflammasome pathways are involved in Prototheca bovis infections of mouse mammary gland tissue and mammary epithelial cells

    DOI: 10.1016/j.freeradbiomed.2022.04.005

    PMID: 35417750

    		 Sample: mammary gl,tissue
  9. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
    Investigation of the impact of rosuvastatin and telmisartan in doxorubicin-induced acute cardiotoxicity

    DOI: 10.1016/j.biopha.2022.113673
    Sample: serum
  10. Frontiers in Cardiovascular Medicine (2021) IF: 6.05
    Silencing of OIP5-AS1 Protects Endothelial Cells From ox-LDL-Triggered Injury by Regulating KLF5 Expression via Sponging miR-135a-5p

    DOI: 10.3389/fcvm.2021.596506
    Sample: HUVECs
  11. Biomedicines (2021) IF: 6.081
    WIN55,212-2 Attenuates Cognitive Impairments in AlCl3 + d-Galactose-Induced Alzheimer’s Disease Rats by Enhancing Neurogenesis and Reversing Oxidative Stress

    DOI: 10.3390/biomedicines9091270
    Sample: brain
  12. Frontiers in Cell and Developmental Biology (2022) IF: 6.081
    Targeting of the Lipid Metabolism Impairs Resistance to BRAF Kinase Inhibitor in Melanoma

    DOI: 10.3389/fcell.2022.927118

    PMID: 35912092

    		 Sample: LM16 cell,LM36 cell
  13. Frontiers in Molecular Biosciences (2022) IF: 6.113
    Biochemical profiling of metabolomics in heavy metal-intoxicated impaired metabolism and its amelioration using plant-based bioactive compound

    DOI: 10.3389/fmolb.2022.1029729

    PMID: 36330218

    		 Sample: liver
  14. Cancers (2020) IF: 6.126
    Beneficial Molecular Adaptations in BRCA-Mutation Carriers by Combined HIT/HIRT Intervention: Results from a Pilot Study

    DOI: 10.3390/cancers12061526

    PMID: 32532068

    		 Sample: Serum
  15. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    A Novel Furocoumarin Derivative, 5-((diethylamino)me-13 thyl)-3-phenyl-7H-furo [3,2-g] chromen-7-one Upregulates Melanin Synthesis via the Activation of cAMP/PKA and MAPKs Signal Pathway: In Vitro and In Vivo Study

    DOI: 10.3390/ijms232214190
    Sample: serum
  16. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 6.291
    Antibacterial effects of nano-decoction iron polysulfide in epididymitis and the systematic evaluation of its toxicity on the reproductive health of male mice

    DOI: 10.1016/j.ecoenv.2022.113184

    PMID: 35032726

    		 Sample: testis
  17. Antioxidants (2021) IF: 6.313
    Time Course of Redox Biomarkers in COVID-19 Pneumonia: Relation with Inflammatory, Multiorgan Impairment Biomarkers and CT Findings

    DOI: 10.3390/antiox10071126

    PMID: 34356359

    		 Sample: plasma
  18. Antioxidants (2021) IF: 6.313
    Antioxidant Effect of a Probiotic Product on a Model of Oxidative Stress Induced by High-Intensity and Duration Physical Exercise

    DOI: 10.3390/antiox10020323

    PMID: 33671691

    		 Sample: serum
  19. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Pentoxifylline and berberine mitigate diclofenac-induced acute nephrotoxicity in male rats via modulation of inflammation and oxidative stress

    DOI: 10.1016/j.biopha.2022.113225

    PMID: 35671584

    		 Sample: serum
  20. Oxidative Medicine and Cellular Longevity (2021) IF: 6.543
    Upregulation of Matrix Metalloproteinase-9 Protects against Sepsis-Induced Acute Lung Injury via Promoting the Release of Soluble Receptor for Advanced Glycation End Products

    DOI: 10.1155/2021/8889313

    PMID: 33628393

    		 Sample: Bronchoalveolar Lavage Fluid
  21. Oxidative Medicine and Cellular Longevity (2021) IF: 6.543
    GSTM1 Modulates Expression of Endothelial Adhesion Molecules in Uremic Milieu

    DOI: 10.1155/2021/6678924

    PMID: 33574979

    		 Sample: Cell lysate
  22. Nutrients (2022) IF: 6.706
    Puerariae lobatae Radix Alleviates Pre-Eclampsia by Remodeling Gut Microbiota and Protecting the Gut and Placental Barriers

    DOI: 10.3390/nu14235025
    Sample: serum,placental
  23. Antioxidants (2020) IF: 5.014
    Short- and Long-Term Effectiveness of Supplementation with Non-Animal Chondroitin Sulphate on Inflammation, Oxidative Stress and Functional Status in Obese Subjects with Moderate Knee Osteoarthritis before and after Physical Stress: A Randomized, Double-B

    DOI: 10.3390/antiox9121241
    Sample: serum
  24. Marine Drugs (2022) IF: 5.118
    Anti-Photoaging Effect of Hydrolysates from Pacific Whiting Skin via MAPK/AP-1, NF-κB, TGF-β/Smad, and Nrf-2/HO-1 Signaling Pathway in UVB-Induced Human Dermal Fibroblasts

    DOI: 10.3390/md20050308
    Sample: Human dermal fibroblasts (HDFs)
  25. INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY (2019) IF: 5.176
    Emodin Rescued Hyperhomocysteinemia-Induced Dementia and Alzheimer’s Disease-Like Features in Rats

    DOI: 10.1093/ijnp/pyy090
    Sample: Plasma,Tissue homogenate
  26. Reproductive Biology and Endocrinology (2021) IF: 5.211
    Hindlimb unloading-induced reproductive suppression via Downregulation of hypothalamic Kiss-1 expression in adult male rats

    DOI: 10.1186/s12958-021-00694-4

    PMID: 33663539

    		 Sample: Tissue homogenate
  27. COLLOIDS AND SURFACES B-BIOINTERFACES (2021) IF: 5.268
    Electrospun nanoyarn and exosomes of adipose-derived stem cells for urethral regeneration: Evaluations in vitro and in vivo

    DOI: 10.1016/j.colsurfb.2021.112218

    PMID: 34801930

    		 Sample: HFFs cells,HSFs cells
  28. Frontiers in Psychiatry (2022) IF: 5.435
    Efficacy of a novel proprietary dietary supplement (TRI 360TM) on psychological symptoms and stress-related quality of life in adult subjects: A randomized controlled clinical trial

    DOI: 10.3389/fpsyt.2022.919284

    PMID: 36032243

    		 Sample: serum
  29. RNA Biology (2019) IF: 5.477
    MicroRNA-142-3p suppresses endometriosis by regulating KLF9-mediated autophagy in vitro and in vivo

    DOI: 10.1080/15476286.2019.1657352

    PMID: 31425004

    		 Sample: Serum,Cell culture medium
  30. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2018) IF: 5.5
    AMPK Hyper-Activation Alters Fatty Acids Metabolism and Impairs Invasiveness of Trophoblasts in Preeclampsia

    DOI: 10.1159/000492995
    Sample: HTR8/SVneo cells
  31. Journal of the American Heart Association (2022) IF: 5.501
    Trimetazidine Alleviates Postresuscitation Myocardial Dysfunction and Improves 96‐Hour Survival in a Ventricular Fibrillation Rat Model

    DOI: 10.1161/JAHA.121.023378
    Sample: plasma
  32. Frontiers in Cellular Neuroscience (2022) IF: 5.505
    14,15-Epoxyeicosatrienoic Acid Protect Against Glucose Deprivation and Reperfusion-Induced Cerebral Microvascular Endothelial Cells Injury by Modulating Mitochondrial Autophagy via SIRT1/FOXO3a Signaling Pathway and TSPO Protein

    DOI: 10.3389/fncel.2022.888836

    PMID: 35558879

    		 Sample: HCMECs
  33. FREE RADICAL BIOLOGY AND MEDICINE (2019) IF: 5.657
    Protective effect of Prunella vulgaris var. L extract against blue light induced damages in ARPE-19 cells and mouse retina

    DOI: 10.1016/j.freeradbiomed.2019.12.003
    Sample: ARPE-19 cell
  34. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    rmMANF prevents sepsis-associated lung injury via inhibiting endoplasmic reticulum stress-induced ferroptosis in mice

    DOI: 10.1016/j.intimp.2022.109608
    Sample: lung tissues
  35. Nutrients (2021) IF: 5.719
    Effects of the Consumption of Low-Fat Cooked Ham with Reduced Salt Enriched with Antioxidants on the Improvement of Cardiovascular Health: A Randomized Clinical Trial

    DOI: 10.3390/nu13051480

    PMID: 33925704

    		 Sample: Serum
  36. Nutrients (2021) IF: 5.719
    Effects of 12 Weeks Cosmos caudatus Supplement among Older Adults with Mild Cognitive Impairment: A Randomized, Double-Blind and Placebo-Controlled Trial

    DOI: 10.3390/nu13020434

    PMID: 33572715

    		 Sample: Serum
  37. Cancer Cell International (2022) IF: 5.722
    BuFeiXiaoJiYin ameliorates the NLRP3 inflammation response and gut microbiota in mice with lung cancer companied with Qi-yin deficiency

    DOI: 10.1186/s12935-022-02543-9

    PMID: 35292015

    		 Sample: serum
  38. Frontiers in Pharmacology (2021) IF: 5.811
    The Anti-Inflammatory Effect of a Combination of Five Compounds From Five Chinese Herbal Medicines Used in the Treatment of COPD

    DOI: 10.3389/fphar.2021.709702
    Sample: serum
  39. PHYTOTHERAPY RESEARCH (2021) IF: 5.882
    Effects of delta-tocotrienol supplementation on Glycemic Control, oxidative stress, inflammatory biomarkers and miRNA expression in type 2 diabetes mellitus: A randomized control trial

    DOI: 10.1002/ptr.7113

    PMID: 33899292

    		 Sample: Serum
  40. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Cardioprotective Effects of a Nonsteroidal Mineralocorticoid Receptor Blocker, Esaxerenone, in Dahl Salt-Sensitive Hypertensive Rats

    DOI: 10.3390/ijms22042069

    PMID: 33669786

    		 Sample: Tissue（left ventricular）
  41. Frontiers in Pharmacology (2022) IF: 5.988
    Anti-arthritic and toxicological evaluation of ethanolic extract of Alternanthera bettzickiana in rats.

    DOI: 10.3389/fphar.2022.1002037
    Sample: serum
  42. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
    Evaluation of testicular torsion management in Ogbomoso, South-Western Nigeria and surgical detorsion-augmented treatment with phytochemical fractions of Corchorus olitorius leaf in experimental rats

    DOI: 10.1016/j.sjbs.2022.103495
    Sample: testicular tissue
  43. INFLAMMATION (2022) IF: 4.092
    TDAG51-Deficiency Podocytes are Protected from High-Glucose-Induced Damage Through Nrf2 Activation via the AKT–GSK-3β Pathway

    DOI: 10.1007/s10753-022-01638-9
    Sample: podocytes
  44. ACS Omega (2022) IF: 4.132
    Comparative Biochemical Profiling of Aluminum Chloride and Sodium Azide Induced Neuroinflammation and Cardiometabolic Disturbance

    DOI: 10.1021/acsomega.2c05467

    PMID: 36385866

    		 Sample: serum
  45. Drug Design Development and Therapy (2021) IF: 4.162
    Role of Oxidative Stress and Reduced Endogenous Hydrogen Sulfide in Diabetic Nephropathy

    DOI: 10.2147/DDDT.S291591

    PMID: 33707940

    		 Sample: Tissue homogenate
  46. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
    Differential neuroprotective effect of curcuminoid formulations in aluminum chloride–induced Alzheimer’s disease

    DOI: 10.1007/s11356-022-20593-4
    Sample: brain
  47. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
    Centella asiatica extract protects against cisplatin-induced hepatotoxicity via targeting oxidative stress, inflammation, and apoptosis

    DOI: 10.1007/s11356-022-18626-z

    PMID: 35029831

    		 Sample: hepatic tissue
  48. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
    Biochemical profiling of lead-intoxicated impaired lipid metabolism and its amelioration using plant-based bioactive compound

    DOI: 10.1007/s11356-022-20069-5

    PMID: 35420345

    		 Sample: serum
  49. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223
    Biochemical investigation of human exposure to aflatoxin M1 and its association with risk factors of diabetes mellitus

    DOI: 10.1007/s11356-021-14871-w

    PMID: 34216342

    		 Sample: serum
  50. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2018) IF: 4.302
    Modulation of the oxidative plasmatic state in gastroesophageal reflux disease with the addition of rich water molecular hydrogen: A new biological vision

    DOI: 10.1111/jcmm.13569
    Sample: Plasma
  51. Drug Design Development and Therapy (2022) IF: 4.319
    The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study

    DOI: 10.2147/DDDT.S344247

    PMID: 35965961

    		 Sample: serum
  52. Scientific Reports (2021) IF: 4.38
    Cacao powder supplementation attenuates oxidative stress, cholinergic impairment, and apoptosis in d-galactose-induced aging rat brain

    DOI: 10.1038/s41598-021-96800-y

    PMID: 34504131

    		 Sample: Serum
  53. EUROPEAN JOURNAL OF PHARMACOLOGY (2021) IF: 4.432
    Glycyrrhizic acid ameliorates myocardial ischemia-reperfusion injury in rats through inhibiting endoplasmic reticulum stress

    DOI: 10.1016/j.ejphar.2021.174353

    PMID: 34274339

    		 Sample: Myocardial
  54. ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2022) IF: 4.478
    Blood serum lipid profiling may improve the management of recurrent miscarriage: a combination of machine learning of mid-infrared spectra and biochemical assays

    DOI: 10.1007/s00216-022-04370-3

    PMID: 36227296

    		 Sample: blood
  55. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Endothelial progenitor cell–derived exosomes ameliorate endothelial dysfunction in a mouse model of diabetes

    DOI: 10.1016/j.biopha.2020.110756
    Sample: Serum
  56. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Cycloastragenol prevents age-related bone loss: Evidence in d-galactose-treated and aged rats

    DOI: 10.1016/j.biopha.2020.110304

    PMID: 32497865

    		 Sample: Serum
  57. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    2,3,5,4'-tetrahydroxystilbence-2-O-β-D-glucoside attenuates hepatic steatosis via IKKβ/NF-κB and Keap1-Nrf2 pathways in larval zebrafish

    DOI: 10.1016/j.biopha.2020.110138
    Sample: Tissue homogenate
  58. Nutrients (2020) IF: 4.546
    The Effects of Tocotrienol-Rich Vitamin E (Tocovid) on Diabetic Neuropathy: A Phase II Randomized Controlled Trial

    DOI: 10.3390/nu12051522

    PMID: 32456230

    		 Sample: Serum
  59. Nutrients (2020) IF: 4.546
    Mikania micrantha Extract Inhibits HMG-CoA Reductase and ACAT2 and Ameliorates Hypercholesterolemia and Lipid Peroxidation in High Cholesterol-Fed Rats

    DOI: 10.3390/nu12103077

    PMID: 33050310

    		 Sample: Serum
  60. International Journal of Environmental Research and Public Health (2022) IF: 4.614
    Effects of an Exercise Program Combining Aerobic and Resistance Training on Protein Expressions of Neurotrophic Factors in Obese Rats Injected with Beta-Amyloid

    DOI: 10.3390/ijerph19137921

    PMID: 35805580

    		 Sample: plasma
  61. Frontiers in Physiology (2022) IF: 4.755
    Abscopal effects of thoracic X-ray radiation on spermatogenesis in mice

    DOI: 10.3389/fphys.2022.984429

    PMID: 36091371

    		 Sample: lung,serum,testis
  62. Biomedicines (2022) IF: 4.757
    Exploiting Bacteria for Improving Hypoxemia of COVID-19 Patients

    DOI: 10.3390/biomedicines10081851

    PMID: 36009399

    		 Sample: plasma
  63. BIOORGANIC CHEMISTRY (2020) IF: 4.831
    Two resveratrol analogs, pinosylvin and 4,4′-dihydroxystilbene, improve oligoasthenospermia in a mouse model by attenuating oxidative stress via the Nrf2-ARE pathway

    DOI: 10.1016/j.bioorg.2020.104295
    Sample: Testicular
  64. PROCESS BIOCHEMISTRY (2022) IF: 4.885
    Optimization of ultrasound-assisted extraction, structural, functional, and antioxidant properties of Glycyrrhiza uralensis seed protein

    DOI: 10.1016/j.procbio.2022.11.001
    Sample: serum,liver
  65. SCIENCE OF THE TOTAL ENVIRONMENT (2017) IF: 4.9
    Long-term exposure to high air pollution induces cumulative DNA damages in traffic policemen

    DOI: 10.1016/j.scitotenv.2017.03.179
    Sample: Serum
  66. Journal of Personalized Medicine (2022) IF: 4.945
    Interplay between Comprehensive Inflammation Indices and Redox Biomarkers in Testicular Germ-Cell Tumors

    DOI: 10.3390/jpm12050833

    PMID: 35629255
  67. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2021) IF: 3
    Limonin modulated immune and inflammatory responses to suppress colorectal adenocarcinoma in mice model

    DOI: 10.1007/s00210-021-02101-6

    PMID: 34009457

    		 Sample: Serum
  68. NEUROSCIENCE LETTERS (2021) IF: 3.046
    Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence

    DOI: 10.1016/j.neulet.2021.136385

    PMID: 34871743

    		 Sample: hippocampus,liver
  69. INFLAMMOPHARMACOLOGY (2020) IF: 3.238
    Perindopril ameliorates experimental Alzheimer’s disease progression: role of amyloid β degradation, central estrogen receptor and hyperlipidemic-lipid raft signaling

    DOI: 10.1007/s10787-020-00724-4
    Sample: Serum,Tissue homogenate
  70. NEUROPEPTIDES (2021) IF: 3.286
    Dynorphin A (1–8) inhibits oxidative stress and apoptosis in MCAO rats, affording neuroprotection through NMDA receptor and κ-opioid receptor channels

    DOI: 10.1016/j.npep.2021.102182
    Sample: serum
  71. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020) IF: 3.292
    Nicotine-mediated upregulation of microRNA-141 expression determines adipokine-intervened insulin resistance

    DOI: 10.1016/j.etap.2020.103506

    PMID: 33002592

    		 Sample: Serum
  72. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020) IF: 3.292
    Mechanistic perspective of protective effects of nilotinib against cisplatin-induced testicular injury in rats: Role of JNK/caspase-3 signaling inhibition

    DOI: 10.1016/j.etap.2020.103334
    Sample: testicular
  73. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020) IF: 3.292
    Chronic exposure of bisphenol A impairs carbohydrate and lipid metabolism by altering corresponding enzymatic and metabolic pathways

    DOI: 10.1016/j.etap.2020.103387

    PMID: 32339907

    		 Sample: Serum
  74. COMPLEMENTARY THERAPIES IN MEDICINE (2022) IF: 3.335
    Comparison of delta-tocotrienol and alpha-tocopherol effects on hepatic steatosis and inflammatory biomarkers in patients with non-alcoholic fatty liver disease: A randomized double-blind active-controlled trial

    DOI: 10.1016/j.ctim.2022.102866

    PMID: 35933083

    		 Sample: serum
  75. EXPERIMENTAL GERONTOLOGY (2017) IF: 3.34
    Dried plum and chokeberry ameliorate d-galactose-induced aging in mice by regulation of Pl3k/Akt-mediated Nrf2 and Nf-kB pathways

    DOI: 10.1016/j.exger.2017.05.004
    Sample: Serum
  76. Frontiers in Veterinary Science (2021) IF: 3.412
    Chronic Exposure to Continuous Brightness or Darkness Modulates Immune Responses and Ameliorates the Antioxidant Enzyme System in Male Rats

    DOI: 10.3389/fvets.2021.621188

    PMID: 33937367

    		 Sample: Serum,Tissue homogenate,Tissue Slice
  77. DISEASE MARKERS (2022) IF: 3.464
    HFpEF and Atrial Fibrillation: The Enigmatic Interplay of Dysmetabolism, Biomarkers, and Vascular Endothelial Dysfunction

    DOI: 10.1155/2022/9539676

    PMID: 36330203

    		 Sample: Plasma
  78. European Review for Medical and Pharmacological Sciences (2022) IF: 3.507
    Protective effect of ethyl pyruvate on amikacin-induced ototoxicity in rats.

    DOI: 10.26355/eurrev_202204_28480
    Sample: cochlear tissue
  79. ACS Omega (2022) IF: 3.512
    Sarcococca saligna Hydroalcoholic Extract Ameliorates Arthritis in Complete Freund’s Adjuvant-Induced Arthritic Rats via Modulation of Inflammatory Biomarkers and Suppression of Oxidative Stress Markers

    DOI: 10.1021/acsomega.2c00619
    Sample: serum
  80. FOOD RESEARCH INTERNATIONAL (2018) IF: 3.52
    The effect of kefir produced from natural kefir grains on the intestinal microbial populations and antioxidant capacities of Balb/c mice

    DOI: 10.1016/j.foodres.2018.10.080

    PMID: 30599959

    		 Sample: Serum
  81. Toxins (2020) IF: 3.531
    Vitamin E-Bonded Membranes Do Not Influence Markers of Oxidative Stress in Hemodialysis Patients with Homozygous Glutathione Transferase M1 Gene Deletion

    DOI: 10.3390/toxins12060352
    Sample: plasma
  82. Therapeutic Advances in Endocrinology and Metabolism (2019) IF: 3.543
    Tocotrienol-rich vitamin E improves diabetic nephropathy and persists 6–9 months after washout: a phase IIa randomized controlled trial:

    DOI: 10.1177/2042018819895462
    Sample: Serum
  83. Food Science & Nutrition (2022) IF: 3.553
    Curcumin-loaded cockle shell-derived calcium carbonate nanoparticles ameliorates lead-induced neurotoxicity in rats via attenuation of oxidative stress

    DOI: 10.1002/fsn3.3096
    Sample: cerebellum,cerebrum
  84. Frontiers in Behavioral Neuroscience (2022) IF: 3.558
    Acupuncture Relieves Stress-Induced Depressive Behavior by Reducing Oxidative Stress and Neuroapoptosis in Rats

    DOI: 10.3389/fnbeh.2021.783056

    PMID: 35058758

    		 Sample: hippocampus tissue
  85. METABOLIC BRAIN DISEASE (2022) IF: 3.584
    Hepatoprotective and neuroprotective effect of taxifolin on hepatic encephalopathy in rats

    DOI: 10.1007/s11011-022-00952-3

    PMID: 35298730

    		 Sample: hippocampal,hepatic
  86. TOXICOLOGY AND APPLIED PHARMACOLOGY (2019) IF: 3.585
    Low molecular polypeptide from oyster hydrolysate recovers photoaging in SKH-1 hairless mice

    DOI: 10.1016/j.taap.2019.114844

    PMID: 31785243

    		 Sample: Serum
  87. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2020) IF: 3.606
    Ameliorative effect of curcumin on lead‐induced hematological and hepatorenal toxicity in a rat model

    DOI: 10.1002/jbt.22483
    Sample: serum
  88. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2022) IF: 3.657
    Early Diagnosis of Coronary Artery Disease by Inflammatory Biomarkers of Atherosclerosis in Patients with Angina

    DOI: 10.1089/jir.2022.0110

    PMID: 36121373

    		 Sample: plasma
  89. BIOGERONTOLOGY (2019) IF: 3.805
    Goat milk attenuates mimetic aging related memory impairment via suppressing brain oxidative stress, neurodegeneration and modulating neurotrophic factors in d -galactose-induced aging model

    DOI: 10.1007/s10522-019-09854-x
    Sample: brain
  90. CLINICAL IMMUNOLOGY (2022) IF: 3.969
    LncRNA SOX2OT facilitates LPS-induced inflammatory injury by regulating intercellular adhesion molecule 1 (ICAM1) via sponging miR-215-5p

    DOI: 10.1016/j.clim.2022.109006

    PMID: 35413439

    		 Sample: AC16 cell
  91. NEUROCHEMICAL RESEARCH (2022) IF: 3.996
    Epigallocatechin-3-Gallate Ameliorated Iron Accumulation and Apoptosis and Promoted Neuronal Regeneration and Memory/Cognitive Functions in the Hippocampus Induced by Exposure to a Chronic High-Altitude Hypoxia Environment

    DOI: 10.1007/s11064-022-03611-2

    PMID: 35552996

    		 Sample: hippocampus
  92. Scientific Reports (2020) IF: 3.998
    Reproductive effects of subchronic exposure to acetamiprid in male rats

    DOI: 10.1038/s41598-020-65887-0

    PMID: 32488017

    		 Sample: Plasma,Tissue homogenate
  93. Scientific Reports (2020) IF: 3.998
    Cyclovirobuxine D protects against diabetic cardiomyopathy by activating Nrf2-mediated antioxidant responses

    DOI: 10.1038/s41598-020-63498-3
    Sample: cardiac tissue
  94. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2020) IF: 2.05
    Metformin attenuated histopathological ocular deteriorations in a streptozotocin-induced hyperglycemic rat model

    DOI: 10.1007/s00210-020-01989-w

    PMID: 33047165

    		 Sample: Serum
  95. MOLECULAR VISION (2017) IF: 2.057
    Pinus densiflora bark extract prevents selenite-induced cataract formation in the lens of Sprague Dawley rat pups

    PMID: 28943754

    		 Sample: lens tissue
  96. MOLECULAR VISION (2019) IF: 2.174
    Polyphenol-enriched fraction of Vaccinium uliginosum L. protects selenite-induced cataract formation in the lens of Sprague-Dawley rat pups

    PMID: 30820147

    		 Sample: lens tissue
  97. Animals (2020) IF: 2.323
    Obesity-Induced Heart Rate Variability Impairment and Decreased Systolic Function in Obese Male Dogs

    DOI: 10.3390/ani10081383

    PMID: 32785083

    		 Sample: Plasma
  98. Journal of Natural Medicines (2021) IF: 2.343
    Dehydroabietic acid improves nonalcoholic fatty liver disease through activating the Keap1/Nrf2-ARE signaling pathway to reduce ferroptosis

    DOI: 10.1007/s11418-021-01491-4

    PMID: 33590347

    		 Sample: Tissue homogenate(liver)
  99. Journal of Pain Research (2020) IF: 2.386
    Oxidative Stress Contributes to Hyperalgesia in Osteoporotic Mice

    DOI: 10.2147/JPR.S234334
    Sample: plasma
  100. Journal of Plastic Reconstructive and Aesthetic Surgery (2020) IF: 2.39
    Hydrogen Rich Saline Reduces Tissue Injury and Improves Skin Flap Survival On A Rat Hindlimb Degloving Injury Model

    DOI: 10.1016/j.bjps.2020.12.045
    Sample: skin
  101. COMPLEMENTARY THERAPIES IN MEDICINE (2022) IF: 2.446
    Role of resveratrol supplementation in regulation of glucose hemostasis, inflammation and oxidative stress in patients with diabetes mellitus type 2: A randomized, placebo-controlled trial

    DOI: 10.1016/j.ctim.2022.102819

    PMID: 35240291

    		 Sample: serum
  102. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Combination of endothelial progenitor cells and BB‑94 significantly alleviates brain damage in a mouse model of diabetic ischemic stroke

    DOI: 10.3892/etm.2021.10221
    Sample: Cell culture supernatant
  103. GERIATRIC NURSING (2022) IF: 2.525
    Dietary diversity, diet quality, and oxidative stress in older adults

    DOI: 10.1016/j.gerinurse.2022.09.013

    PMID: 36219935

    		 Sample: blood
  104. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY (2016) IF: 2.557
    A novel translocator protein 18 kDa ligand, ZBD‐2, exerts neuroprotective effects against acute spinal cord injury

    DOI: 10.1111/1440-1681.12606
    Sample: Serum
  105. NEUROCHEMICAL RESEARCH (2017) IF: 2.581
    Antioxidative and Anti-Apoptotic Roles of Silibinin in Reversing Learning and Memory Deficits in APP/PS1 Mice

    DOI: 10.1007/s11064-017-2389-3
    Sample: Tissue homogenate
  106. Dose-Response (2022) IF: 2.623
    Correlation of Ionized Magnesium with the Parameters of Oxidative Stress as Potential Biomarkers in Patients with Anxiety and Depression: A Pilot Study:

    DOI: 10.1177/15593258221116741
    Sample: serum
  107. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Qindan Capsule Attenuates Myocardial Hypertrophy and Fibrosis in Pressure Overload-Induced Mice Involving mTOR and TGF-β1/Smad Signaling Pathway Inhibition

    DOI: 10.1155/2021/5577875
    Sample: Tissue homogenate
  108. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
    Etlingera elatior Flower Aqueous Extract Protects against Oxidative Stress-Induced Nephropathy in a Rat Model of Type 2 Diabetes

    DOI: 10.1155/2022/2814196

    PMID: 35502173

    		 Sample: plasma
  109. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Antioxidant Activity of Sprouts Extracts Is Correlated with Their Anti-Obesity and Anti-Inflammatory Effects in High-Fat Diet-Fed Mice

    DOI: 10.1155/2021/8367802

    PMID: 33643424

    		 Sample: Plasma
  110. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
    San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

    DOI: 10.1155/2022/7832540
    Sample: hepatic tissue
  111. Dose-Response (2022) IF: 2.658
    Pharmacological Evaluation of Mentha piperita Against Urolithiasis: An In Vitro and In Vivo Study:

    DOI: 10.1177/15593258211073087
    Sample: kidney
  112. NUTRITION RESEARCH (2018) IF: 2.707
    Additive effect of walnut and chokeberry on regulation of antioxidant enzyme gene expression and attenuation of lipid peroxidation in d-galactose-induced aging-mouse model

    DOI: 10.1016/j.nutres.2018.09.011
    Sample: Serum
  113. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 2.72
    Watermelon (Citrullus lanatus) leaf extract attenuates biochemical and histological parameters in high-fat diet/streptozotocin-induced diabetic rats

    DOI: 10.1111/jfbc.14058

    PMID: 34981526

    		 Sample: serum
  114. BMC Pulmonary Medicine (2018) IF: 2.721
    NOX4 expression and distal arteriolar remodeling correlate with pulmonary hypertension in COPD

    DOI: 10.1186/s12890-018-0680-y
    Sample: Serum
  115. IUBMB LIFE (2014) IF: 2.755
    2,3,5,4′‐tetrahydroxystilbene‐2‐O‐β‐d‐glucoside protects human umbilical vein endothelial cells against lysophosphatidylcholine‐induced apoptosis by upregulating superoxide dismutase and glutathione peroxidase

    DOI: 10.1002/iub.1321
    Sample: Cell lysate
  116. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2020) IF: 2.802
    Curcumin-loaded cockle shell-derived calcium carbonate nanoparticles: A novel strategy for the treatment of lead-induced hepato-renal toxicity in rats

    DOI: 10.1016/j.sjbs.2020.03.009

    PMID: 32489292

    		 Sample: Serum
  117. BMC Complementary and Alternative Medicine (2020) IF: 2.833
    The effects of six months Persicaria minor extract supplement among older adults with mild cognitive impairment: a double-blinded, randomized, and placebo-controlled trial

    DOI: 10.1186/s12906-020-03092-2

    PMID: 33076878

    		 Sample: Serum
  118. ACS Omega (2020) IF: 2.87
    Antiarthritic Potential of Comprehensively Standardized Extract of Alternanthera bettzickiana: In Vitro and In Vivo Studies

    DOI: 10.1021/acsomega.0c01670

    PMID: 32803042

    		 Sample: Serum
  119. SAUDI PHARMACEUTICAL JOURNAL (2020) IF: 2.879
    Possible combined effect of perindopril and Azilsartan in an experimental model of dementia in rats

    DOI: 10.1016/j.jsps.2020.03.009
    Sample: Tissue homogenate
  120. BRAIN RESEARCH (2019) IF: 2.929
    Effect of enriched environment and predictable chronic stress on spatial memory in adolescent rats: Predominant expression of BDNF, nNOS, and interestingly malondialdehyde in the right hippocampus

    DOI: 10.1016/j.brainres.2019.146326
    Sample: hippocampus
  121. Molecular Medicine Reports (2021) IF: 2.952
    Remote ischemic post‑conditioning alleviates ischemia/reperfusion‑induced intestinal injury via the ERK signaling pathway‑mediated RAGE/HMGB axis

    DOI: 10.3892/mmr.2021.12413

    PMID: 34490475

    		 Sample: serum
  122. Molecular Medicine Reports (2018) IF: 2.952
    Puerarin reduces ischemia/reperfusion-induced myocardial injury in diabetic rats via upregulation of vascular endothelial growth factor A/angiotensin-1 and suppression of apoptosis

    DOI: 10.3892/mmr.2018.8754
    Sample: plasma
  123. PeerJ (2022) IF: 2.984
    Quercetin prevents chronic kidney disease on mesangial cells model by regulating inflammation, oxidative stress, and TGF-β1/SMADs pathway

    DOI: 10.7717/peerj.13257

    PMID: 35673387

    		 Sample: SV40 MES 13 cell
  124. Romanian Journal of Morphology and Embryology (2021) IF: 1.033
    Carvacrol treatment opens Kir6.2 ATP-dependent potassium channels and prevents apoptosis on rat testis following ischemia–reperfusion injury model

    DOI: 10.47162/RJME.62.1.17
    Sample: serum
  125. TRANSPLANTATION PROCEEDINGS (2021) IF: 1.066
    Administration of Donor-Derived Nonexpanded Adipose Stromal Vascular Fraction Attenuates Ischemia-Reperfusion Injury in Donation After Cardiac Death Rat Renal Transplantation

    DOI: 10.1016/j.transproceed.2021.05.012

    PMID: 34266654

    		 Sample: serum
  126. Annals of Surgical Treatment and Research (2019) IF: 1.181
    Examination of protective and therapeutic effects of ruscogenin on cerulein-induced experimental acute pancreatitis in rats

    DOI: 10.4174/astr.2019.97.6.271

    PMID: 31824881

    		 Sample: Serum,Tissue homogenate
  127. Brazilian Journal of Pharmaceutical Sciences (2022) IF: 1.214
    Stingless bee propolis, metformin, and their combination alleviate diabetic cardiomyopathy

    DOI: 10.1590/s2175-97902022e19652
    Sample: Heart tissue
  128. Anatolian Journal of Cardiology (2018) IF: 1.271
    Protective effects of curcumin and beta-carotene on cisplatin-induced cardiotoxicity: An experimental rat model

    DOI: 10.14744/AnatolJCardiol.2018.53059
    Sample: Tissue homogenate
  129. Journal of Zhejiang University-SCIENCE B (2016) IF: 1.303
    Myricetin protects against diet-induced obesity and ameliorates oxidative stress in C57BL/6 mice

    DOI: 10.1631/jzus.B1600074
    Sample: Serum
  130. JOURNAL OF INVESTIGATIVE SURGERY (2017) IF: 1.532
    Ozone Partially Decreases Axonal and Myelin Damage in an Experimental Sciatic Nerve Injury Model

    DOI: 10.1080/08941939.2017.1369606
    Sample: Serum
  131. Molecular Medicine Reports (2015) IF: 1.554
    Asiaticoside attenuates the effects of spinal cord injury through antioxidant and anti‑inflammatory effects, and inhibition of the p38‑MAPK mechanism

    DOI: 10.3892/mmr.2015.4425
    Sample: Serum
  132. Molecular Medicine Reports (2016) IF: 1.559
    Nobiletin ameliorates isoflurane-induced cognitive impairment via antioxidant, anti-inflammatory and anti-apoptotic effects in aging rats

    DOI: 10.3892/mmr.2016.5919

    PMID: 27840933

    		 Sample: Serum
  133. TOXICOLOGY AND INDUSTRIAL HEALTH (2019) IF: 1.635
    Toxic effects of subchronic oral acetamiprid exposure in rats:

    DOI: 10.1177/0748233719893203
    Sample: plasma,renal,liver
  134. JOURNAL OF FOOD BIOCHEMISTRY (2020) IF: 1.662
    Citrus hystrix leaf extract attenuated diabetic‐cataract in STZ‐rats

    DOI: 10.1111/jfbc.13258
    Sample: serum
  135. Molecular Medicine Reports (2017) IF: 1.692
    AMPK activation restores ischemic post‑conditioning cardioprotection in STZ‑induced type 1 diabetic rats: Role of autophagy

    DOI: 10.3892/mmr.2017.7033
    Sample: Serum
  136. ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS (2022) IF: 1.753
    Protective effect by low-intensity downhill running training against muscle damage and oxidative stress after high-intensity downhill running in rats

    DOI: 10.1590/0001-3765202220200265

    PMID: 35507975

    		 Sample: Plasma,vastus intermedius muscle tissue
  137. CURRENT EYE RESEARCH (2020) IF: 1.754
    Expression of α-Klotho Is Downregulated and Associated with Oxidative Stress in the Lens in Streptozotocin-induced Diabetic Rats

    DOI: 10.1080/02713683.2020.1805768

    PMID: 32744464

    		 Sample: Tissue homogenate
  138. Brazilian Journal of Otorhinolaryngology (2022) IF: 1.811
    Astaxanthin protects against hearing impairment in diabetic rats

    DOI: 10.1016/j.bjorl.2022.02.004

    PMID: 35331657

    		 Sample: serum,cochlea
  139. Cutaneous and Ocular Toxicology (2021) IF: 1.82
    Effects of Achillea millefolium on cisplatin induced ocular toxicity: an experimental study

    DOI: 10.1080/15569527.2021.1919137

    PMID: 34180746

    		 Sample: ocular
  140. EUROPEAN JOURNAL OF MEDICAL RESEARCH (2020) IF: 1.826
    Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function

    DOI: 10.1186/s40001-020-00431-4

    PMID: 32738910

    		 Sample: Tissue homogenate
  141. Molecular Medicine Reports (2019) IF: 1.851
    Lipid storage droplet protein 5 reduces sodium palmitate‑induced lipotoxicity in human normal liver cells by regulating lipid metabolism‑related factors

    DOI: 10.3892/mmr.2019.10360
    Sample: LO2 cells
  142. BIOMEDICAL AND ENVIRONMENTAL SCIENCES (2019) IF: 1.917
    Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice

    DOI: 10.3967/bes2018.101
    Sample: Plasma,Tissue homogenate
  143. MEDICAL SCIENCE MONITOR (2020) IF: 1.918
    Pyrroloquinoline Quinone Inhibits Oxidative Stress in Rats with Diabetic Nephropathy

    DOI: 10.12659/MSM.924372
    Sample: kidney tissue,NRK-52E cell
  144. Molecular Medicine Reports (2018) IF: 1.922
    Embelin inhibits abdominal aortic aneurysm through decreasing IL‑6‑induced STAT3 and NF‑κB inactivation

    DOI: 10.3892/mmr.2018.9221
    Sample: Aortic tissues
  145. ANTICANCER RESEARCH (2020) IF: 1.994
    The Rectus Sheath Block (RSB) Analgesia Following Laparotomy Could Affect Malonidialdehyde (MDA) Concentrations in Benign Disease and Cancer

    DOI: 10.21873/anticanres.13947

    PMID: 31892574

    		 Sample: Plasma
  146. ANTICANCER RESEARCH (2020) IF: 1.994
    The Induction of Antioxidant Catalase Enzyme With Decrease of Plasma Malonidialdehyde: An Important Reactive Oxidative Species Inhibiting Mechanism

    DOI: 10.21873/anticanres.14584

    PMID: 32988895

    		 Sample: Plasma
  147. Indian Heart Journal (2017)
    The role of polysaccharide peptide of Ganoderma lucidum as a potent antioxidant against atherosclerosis in high risk and stable angina patients

    DOI: 10.1016/j.ihj.2017.12.007
    Sample: plasma
  148. Journal of Diabetes and Metabolic Disorders (2022)
    Protective effects of Labisia pumila against neuropathy in a diabetic rat model

    DOI: 10.1007/s40200-021-00905-0
    Sample: serum
  149. International Journal of Rheumatology (2020)
    Correlation between C-reactive Protein with Malondialdehyde in Systemic Lupus Erythematosus Patients

    DOI: 10.1155/2020/8078412

    PMID: 32695177

    		 Sample: Serum
  150. Advances in Traditional Medicine (2020)
    Anti-hypernociceptive effects of methanol extract of Boswellia dalzielii on STZ-induced diabetic neuropathic pain

    DOI: 10.1007/s13596-019-00411-y
    Sample: serum,sciatic nerves
  151. POLISH JOURNAL OF VETERINARY SCIENCES (2022) IF: 0.859
    Vitamin E protected the mouse adrenal gland against immobilization stress.

    DOI: 10.24425/pjvs.2022.142029
    Sample: adrenal tissue
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Reviews/Q&A

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Q

N****** Submitted [ Dec 11 2023 ]

Asked: Thank you for your response. I detected protein concentration by bradford assay resulting in concentration of (example: 61, 225, 190 mg/ml). How can I normalise protein concentration? Should it be within nanorange to be determined by the kit?

Reply

A

adminSubmitted [ Dec 11 2023 ]

Answered: The tissue samples were made into 10% tissue homogenate, and then the total concentration was detected by BCA, and then ELISA was performed. The concentration of target protein/total tissue protein detected by ELISA method can be obtained the concentration of target protein in the tissue sample (pg/mg prot). It is our suggestion that we give to you. If you have the calculation of other detection methods, you can also search the relevant information for reference calculation.

Q

N****** Submitted [ Dec 11 2023 ]

Asked: After dilution and homogenization of the sample with 1:9 PBS, we need to measure the protein concentration in the sample. I am curious as to why this step is necessary.

Reply

A

adminSubmitted [ Dec 11 2023 ]

Answered: Hi Nourhan, We just suggest that you can choose to detect the protein concentration, you can choose according to your own experimental needs. Because at present many indicators for detecting tissue homogenates in the literature are required for protein quantification, this suggestion is to make the experimental results of the customer in the publication of the article more complete.

A

adminSubmitted [ Dec 11 2023 ]

Answered: Because of the different sizes of the tissue blocks and the efficiency of protein extraction, the total protein content of the tissue homogenates extracted from different samples varies within a certain range, or the protein content varies significantly between samples. These can make subsequent data processing difficult, and it is not possible to distinguish whether the variability is due to the effect of the study treatment or to the protein content. Therefore, the protein quantification of tissue homogenate is highly recommend. This ensures that data from different groups of samples are compared on the same baseline, resulting in a more accurate presentation of the data.

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