MDA(Malondialdehyde) ELISA Kit (E-EL-0060)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal MDA. During the reaction, Universal MDA in the sample or standard competes with a fixed amount of Universal MDA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal MDA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal MDA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Malondialdehyde (MDA) is an end-product of the radicalinitiated oxidative decomposition of poly-unsaturated fatty acids and, therefore, it is a frequently measured biomarker of oxidative stress . More information about reactive carbonyls and proposed intermediates can be found in . In addition, MDA is generated as a side product of thromboxane A2 synthesis and by gamma irradiation of DNA . In case of thromboxane A2 synthesis, MDA is formed during the conversion of the endoperoxide prostaglandin H2 (PGH2) by thromboxane synthase . PGH2 is derived from arachidonic acid by conversion via cyclooxygenases .