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Ca2+-ATPase Activity Assay Kit

Cat:E-BC-K212-S
Manual MSDS

Price: $ 260

Price: $ 180

Size:
100Assays 50Assays
Quantity:
  • Sample Type: animal tissue
  • Detection Instrument: Spectrophotometer (660nm)
  • Reasearch Areas: Oxidative stress
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Product Details
Detection Principle ATPase exists on the membrane of tissue cells and organelles. It is a kind of protease on the biological membrane which plays an important role in material transport, energy conversion and information transmission. Ca2+ which participates in the regulation of different enzyme systems and cell activities plays many important roles in cells. The flow of Ca2+ depends on the Ca2+ -ATPase on the cell membrane, and Ca2+ -ATPase consumes ATP to generate the energy needed for ion transport.
Synonyms Ca<sup>2+</sup>-ATPase
Sample Type animal tissue
Sensitivity 0.8 U/g wet weight
Detection Range 0.8-41 U/g wet weight
Detection Method Colorimetric method
Assay Time 50 min
Precision inter-assay CV: 8.8% | intra-assay CV: 4.1%
Other Reagents Required Normal saline (0.9% NaCl)
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
10% Rat liver tissue homogenate 5-8
10% Rat heart tissue homogenate 5-8
10% Rat kidney tissue homogenate 5-8
10% Mouse liver tissue homogenate 1
10% Rat lung tissue homogenate 5-8
10% Rat brain tissue homogenate 2-3
The diluent is normal saline (0.9% NaCl). For the dilution of other sample types, please do pretest to confirm the dilution factor.