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Monoamine Oxidase (MAO) Activity Assay Kit

  • Cat.No.:E-BC-K008-M

  • Detection instrument: Microplate reader(345 nm-360 nm,optimum wavelength: 355 nm)

To Purchase E-BC-K008-M

Size:
  • 96T
  • 48T
Price: $260
Qty:

Detection principle

MAO can catalyze 4-dimethylambenzylamine to produce p-dimethylaminobenzaldehyde. p-Dimethylaminobenzaldehyde has a characteristic absorption peak at 355 nm. The activity of MAO can be calculated indirectly by analyzing the production of p-dimethylaminobenzaldehyde.

Performance characteristics

Synonyms MAO
Sample type Serum, plasma, animal tissue
Sensitivity 16 U/L
Detection range 16 – 641 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 40 min
Precision Average inter-assay CV: 6%Average intra-assay CV: 3.3%
Other instruments required Centrifuge Micropipette, Incubator
Storage 2-8℃
Valid period 3 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (16 – 641 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Mouse liver tissue homogenate

1

10% Rat liver tissue homogenate

1

10% Rat lung tissue homogenate

1

10% Rat kidney tissue homogenate

1

10% Mouse brain tissue homogenate

1

Human serum

1

Note: The diluent is reagent 3 working solution.

Citations

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