MAO can catalyze 4-dimethylambenzylamine to produce p-dimethylaminobenzaldehyde. p-Dimethylaminobenzaldehyde has a characteristic absorption peak at 355 nm. The activity of MAO can be calculated indirectly by analyzing the production of p-dimethylaminobenzaldehyde.

Performance characteristics

Synonyms	MAO
Sample type	Animal tissue
Sensitivity	16 U/L
Detection range	16 – 641 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	40 min
Precision	Average inter-assay CV: 6%Average intra-assay CV: 3.300%
Other instruments required	Centrifuge Micropipette, Incubator
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (16 – 641 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Mouse liver tissue homogenate	1
10% Rat liver tissue homogenate	1
10% Rat lung tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Mouse brain tissue homogenate	1
Human serum	1

Note: The diluent is reagent 3 working solution.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022) IF: 5.895
Screening of Monoamine Oxidase B Inhibitors from Fragaria nubicola by Ligand Fishing and Their Neuroprotective Effects

DOI: 10.1021/acs.jafc.2c06630

Sample: PC12 cells

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