Mouse β-EP(Beta-Endorphin) ELISA Kit (E-EL-M0184)

Name: Mouse β-EP(Beta-Endorphin) ELISA Kit
Brand: Elabscience
SKU: E-EL-M0184
Price: 150.0 USD
Availability: InStock

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Mouse β-EP(Beta-Endorphin) ELISA Kit - 1

Size	Price	Qty
96T	$ 495.00	- +
48T	$ 396.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	50 μL
Total Assay Time	2 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse β-EP in samples.No significant cross-reactivity or interference between Mouse β-EP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	0.196	0.196	0.196	-
500	0.313	0.297	0.305	-
250	0.510	0.528	0.519	-
125	0.800	0.804	0.802	-
62.5	1.164	1.164	1.164	-
31.25	1.577	1.565	1.571	-
15.63	1.867	1.913	1.890	-
0	2.285	2.379	2.332	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	55.190	144.030	445.270	57.210	144.600	411.150
Standard deviation	2.280	6.450	28.850	4.040	12.990	28.200
CV（%）	4.140	4.480	6.480	7.060	8.980	6.860

Recovery

The recovery of Mouse β-EP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-105	96
EDTA plasma （n=8）	85-101	92
Cell culture media （n=8）	95-107	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse β-EP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-97	93-103	91-105
1:2	Average	91	98	98
1:4	Range	100-114	86-102	83-97
1:4	Average	106	93	90
1:8	Range	94-106	86-101	86-100
1:8	Average	99	93	93

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	86.73-96.30
Sample 2（n=16）	92.00-104.50

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse β-EP. During the reaction, Mouse β-EP in the sample or standard competes with a fixed amount of Mouse β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

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