Mouse ANG1(Angiopoietin 1) ELISA Kit (E-EL-M3014)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ANG1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ANG1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ANG1. You can calculate the concentration of Mouse ANG1 in the samples by comparing the OD of the samples to the standard curve.

Angiopoietin-1 (Ang-1) is a secreted glycoprotein best known for its putative roles in vasculardevelopment (1). It is a member of a family of growth factors that, in humans, also includesAng-2 and Ang-4. Ang-3 appears to be the mouse counterpart to human Ang-4 (2). Ang-1 is aputative 498 amino acid (aa) protein with prominent structural features that includes anN-terminal coiled-coil domain and a C-terminal fibrinogen-like domain (3). The human andmouse proteins exhibit approximately 97% aa sequence identity (3). Ang-1 and Ang-2 exhibitapproximately 60% aa sequence identity and both share the receptor tyrosine kinase Tie-2(3, 4). Integrins may also mediate Ang-1 and Ang-2 activities (5). At least three alternativelyspliced variants of Ang-1 exist, two of which are unable to activate Tie-2 and may act asendogenous suppressors of Ang-1 activity (6). Ang-1 is expressed widely in the mouse embryoand in adult human tissues, primarily by endothelial support cells, as well as megakaryocytesand platelets (4, 7). Ang-1 is thought to exist as a homo-trimer or in higher order homooligomeric complexes (8). It may also form heteromeric complexes with Ang-2 (8).Ang-1 is a positive regulator of blood vessel development, remodeling, and maturation. It is asurvival factor for endothelial cells (ECs) and supports the recruitment of ECs and periendothelial support cells (9-13). Ang-1/Tie-2-mediated cell survival may require the activity ofPI3K/AKT signaling, while migration potentially involves several kinases including PI3K, FAK,and PAK (14-17). Ang-1/Tie-2-mediated sprouting is accompanied by the activation of PI3K,FAK, and secretion of the proteases Plasmin and MMP-2 (18).In vivo, deletion of the Ang-1 gene is embryonic lethal and is accompanied by cardiac defectsand a generalized decrease in vascular complexity (19).