Mouse GAL3 (Galectin 3) CLIA Kit (E-CL-M0319)

Name: Mouse GAL3 (Galectin 3) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0319
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse GAL3 in samples.No significant cross-reactivity or interference between Mouse GAL3 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	54219.000	55629.000	54924.000	54891
400	21466.000	25054.000	23260.000	23227
200	10372.000	9112.000	9742.000	9709
100	4552.000	5144.000	4848.000	4815
50	2394.000	2102.000	2248.000	2215
25	1191.000	1091.000	1141.000	1108
12.5	503.000	523.000	513.000	480
0	33.000	33.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	37.130	69.320	386.860	37.920	66.930	392.570
Standard deviation	3.070	8.010	30.720	4.670	5.150	40.910
CV（%）	8.270	11.560	7.940	12.320	7.690	10.420

Recovery

The recovery of Mouse GAL3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-102	95
EDTA plasma （n=8）	92-107	98
Cell culture media （n=8）	91-104	97

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse GAL3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-107	100-114	101-114
1:2	Average	101	107	108
1:4	Range	85-98	88-100	99-113
1:4	Average	91	93	107
1:8	Range	101-116	94-109	98-114
1:8	Average	107	102	104

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GAL3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse GAL3. You can calculate the concentration of Mouse GAL3 in the samples by comparing the RLU value of the samples to the standard curve.

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