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Mouse GC(Glucagon) ELISA Kit

  • Cat.No.:E-EL-M0555

  • Reactivity: Mouse

To Purchase E-EL-M0555

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes GC in samples. No significant cross-reactivity or interference between GC and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of GC concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GC. During the reaction, GC in the sample or standard competes with a fixed amount of GC on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GC. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GC in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level GC were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level GC were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 108.40 253.50 739.40 98.90 254.20 733.70
Standard deviation 5.60 12.70 31.80 6.60 14.20 32.30
CV (%) 5.17 5.01 4.30 6.67 5.59 4.40


The recovery of GC spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-110 100
EDTA plasma(n=8) 92-102 97
Cell culture media(n=8) 87-100 92


Samples were spiked with high concentrations of Mouse GC and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P55095
SynonymsGCG, GLP1, GLP2, GRPP
Research AreaCancer, Metabolism, Developmental biology, Signal transduction, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

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  1. CELL (2023) IF: 66.849
    Light modulates glucose metabolism by a retina-hypothalamus-brown adipose tissue axis

    DOI: 10.1016/j.cell.2022.12.024

    PMID: 36669474

    Sample: Serum
  2. DIABETES (2016) IF: 8.7840
    Insulin-like growth factor binding protein (IGFBP)-1 could through its RGD domain improve glucose regulation and insulin sensitivity

    DOI: 10.2337/db16-0997

    Sample: Plasma
  3. Frontiers in Nutrition (2022) IF: 6.576
    Slowly Digestible Carbohydrate Diet Ameliorates Hyperglycemia and Hyperlipidemia in High-Fat Diet/Streptozocin-Induced Diabetic Mice

    DOI: 10.3389/fnut.2022.854725

    PMID: 35495933

    Sample: liver tissue
  4. ENDOCRINOLOGY (2023) IF: 5.051
    Aspirin suppresses hepatic glucagon signaling through decreasing production of thromboxane A2

    DOI: 10.1210/endocr/bqac217

    Sample: plasma,liver
  5. Journal of Functional Foods (2022) IF: 5.223
    Arjunolic acid from Cyclocarya paliurus selectively inhibits glucagon secretion from α cells and ameliorates diabetes via ephrin-A1 and EphA4 interaction

    DOI: 10.1016/j.jff.2022.105323

    Sample: serum
  6. NEUROPEPTIDES (2020) IF: 3.286
    GLP-1 preserves β cell function via improvement on islet insulin signaling in high fat diet feeding mice

    DOI: 10.1016/j.npep.2020.102110

    PMID: 33307381

    Sample: serum
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