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Cat.No.:E-EL-M0048
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 15.63-1000 pg/mL |
Sensitivity | 9.38 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Mouse IFN-γ in samples. No significant cross-reactivity or interference between Mouse IFN-γ and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Mouse IFN-γ concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IFN-γ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IFN-γ were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 51.50 | 96.50 | 497.20 | 53.30 | 95.50 | 533.30 |
Standard deviation | 3.20 | 4.40 | 20.90 | 3.70 | 4.70 | 17.10 |
CV (%) | 6.21 | 4.56 | 4.20 | 6.94 | 4.92 | 3.21 |
The recovery of Mouse IFN-γ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 89-103 | 96 |
EDTA plasma (n=8) | 91-103 | 98 |
Cell culture media (n=8) | 96-109 | 102 |
Samples were spiked with high concentrations of Mouse IFN-γ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P01580
|
Synonyms | IFNG, IFG, IFI, Type II Interferon |
Research Area | Cancer, Immunology, Stem cells |
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1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
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PMID: 36715052 |
Sample: bone-marrow-derived dendritic cells (BMDCs),Spleni |
PMID: 33239389 |
Sample: Cell culture supernatant |
Sample: Bone marrow-derived dendritic cell (BMDC) |
Sample: serum |
Sample: B16-F10 cell-xenograft,CT-26 cell-xenograft |
Sample: Lymphocytes |
Sample: serum |
PMID: 35086948 |
Sample: Pancreatic ductal adenocarcinoma (PDAC) tumor tiss |
PMID: 35738798 |
Sample: B16F10 cell-xenograft |
Sample: serum |
PMID: 36314479 |
Sample: Hepa 1-6 cells-xenograft |
PMID: 35168608 |
Sample: tumor cell |
Sample: Serum |
DOI: 10.1111/bph.16061 |
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Sample: Plasma |
DOI: 10.1002/path.6073 |
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Sample: serum |
PMID: 35355680 |
Sample: serum |
PMID: 34029575 |
Sample: Serum,Tissue homogenate |
DOI: 10.7150/thno.45028 |
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PMID: 32685027 |
Sample: Serum |
PMID: 35860242 |
Sample: serum |
Sample: serum |
Sample: interstitial fluid |
DOI: 10.1155/2023/2894695 |
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Sample: colonic tissue |
PMID: 36115109 |
Sample: colon tissue |
Sample: Spleen cells |
PMID: 34659199 |
Sample: plasma,prostate tissue |
PMID: 34691022 |
Sample: serum |
Sample: serum |
Sample: 4T1 cell-xenograft |
PMID: 35935244 |
Sample: splenocytes |
PMID: 34439524 |
Sample: plasma |
DOI: 10.1039/D2FO00777K |
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PMID: 35920097 |
Sample: serum |
DOI: 10.2147/IJN.S318363 |
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Sample: serum,CD8+ T cell,BMDCs |
DOI: 10.1155/2022/2030818 |
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Sample: serum |
Sample: Serum |
Sample: serum,lymphocytes |
DOI: 10.3390/md20050312 |
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PMID: 35621963 |
Sample: hepatic |
PMID: 32553971 |
Sample: Cell sample |
Sample: Tissue homogenate |
PMID: 36218263 |
Sample: BALF,BEAS-2B cells |
Sample: serum |
PMID: 33974424 |
Sample: Cell culture supernatant |
PMID: 35167294 |
Sample: aortic,Raw264.7 cell |
PMID: 34923234 |
Sample: H22 cell-xenograft |
DOI: 10.1159/000493455 |
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Sample: CD4+T helper 1 cells(Th1 cells),bone marrow monocy |
PMID: 36034656 |
Sample: 4T1 cell |
Sample: Bronchoalveolar Lavage Fluid,Tissue homogenate |
DOI: 10.3390/nu13093209 |
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PMID: 34579088 |
Sample: Splenocyte |
DOI: 10.1002/clt2.12114 |
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PMID: 35079347 |
Sample: bronchoalveolar lavage fluid (BALF),Lung tissue |
Sample: Cell culture supernatant |
PMID: 36253573 |
Sample: serum |
PMID: 31632527 |
Sample: serum |
Sample: Cell culture supernatant |
Sample: Serum |
PMID: 33383792 |
Sample: colon tissue,serum |
PMID: 35249190 |
Sample: Bronchoalveolar lavage fluid (BALF) |
DOI: 10.1002/pros.24214 |
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PMID: 34418127 |
Sample: serum |
DOI: 10.1002/pros.24205 |
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PMID: 34320251 |
Sample: serum |
DOI: 10.1002/jsfa.12173 |
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Sample: serum,colon tissue |
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Sample: Cell culture supernatant |
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Sample: Serum,Cell culture supernatant |
Sample: skin |
DOI: 10.1038/srep42655 |
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Sample: Bronchoalveolar Lavage Fluid,Serum |
PMID: 35322143 |
Sample: brain |
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Sample: Serum |
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Sample: skin lesion |
DOI: 10.1111/jcmm.15434 |
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Sample: Plasma |
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PMID: 31963703 |
Sample: Serum,Cell culture supernatant |
Sample: Ulcerative colitis (UC) tissue |
Sample: Serum,Tissue homogenate |
PMID: 33330069 |
Sample: splenocytes |
Sample: hepatic |
PMID: 34768129 |
Sample: splenic lymphocytes |
PMID: 34678694 |
Sample: BALF |
Sample: Cell culture supernatant,Tissue homogenate,serum |
Sample: serum |
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Sample: liver tissue |
DOI: 10.1039/C7FO01161J |
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Sample: serum |
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Sample: Serum |
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Sample: Serum |
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Sample: Tissue homogenate |
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Sample: Plasma,Tissue homogenate |
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Sample: serum,nasal lavage fluid |
Sample: Bronchoalveolar Lavage Fluid |
PMID: 30391706 |
Sample: Serum,Tissue homogenate |
Sample: Tissue homogenate |
PMID: 35965334 |
Sample: Raw 264.7 cell |
PMID: 30772517 |
Sample: Tissue homogenate |
Sample: Serum |
Sample: serum |
DOI: 10.1155/2020/6947482 |
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PMID: 32256194 |
Sample: Serum,Cell culture supernatant,Aqueous Humor |
Sample: Serum,lung tissue |
Sample: Bronchoalveolar Lavage Fluid |
Sample: Serum |
Sample: Serum,Tissue homogenate |
Sample: Tissue homogenate |
PMID: 36152079 |
Sample: Spleen cell |
PMID: 33929783 |
Sample: Tissue homogenate(liver) |
Sample: Serum,Cell culture supernatant |
Sample: serum,small intestinal tissues |
DOI: 10.1155/2022/5394514 |
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PMID: 35178105 |
Sample: plasma |
DOI: 10.1155/2022/7421265 |
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PMID: 35178108 |
Sample: serum |
PMID: 33947419 |
Sample: Serum |
Sample: serum |
PMID: 32781100 |
Sample: Serum,Cell culture supernatant |
Sample: Serum |
PMID: 31209730 |
Sample: Serum |
Sample: BMDCs |
DOI: 10.3892/or.2017.6094 |
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Sample: serum |
DOI: 10.1292/jvms.22-0203 |
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PMID: 36216558 |
Sample: serum |
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Sample: Tissue homogenate |
DOI: 10.1292/jvms.21-0298 |
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PMID: 34393140 |
Sample: serum |
DOI: 10.1111/jfs.12465 |
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Sample: plasma |
PMID: 26884954 |
Sample: lymphocyte |
PMID: 34143400 |
Sample: serum |
Sample: serum |
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Sample: Serum,Tissue homogenate |
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Sample: Plasma,Tissue homogenate |
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