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Mouse IFN-γ(Interferon Gamma) ELISA Kit

Citations(119)Uniprot : P01580

  • Cat.No.:E-EL-M0048

  • Reactivity: Mouse
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IFN-γ in samples. No significant cross-reactivity or interference between Mouse IFN-γ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IFN-γ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFN-γ. You can calculate the concentration of Mouse IFN-γ in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IFN-γ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IFN-γ were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 51.50 96.50 497.20 53.30 95.50 533.30
Standard deviation 3.20 4.40 20.90 3.70 4.70 17.10
CV (%) 6.21 4.56 4.20 6.94 4.92 3.21

Recovery

The recovery of Mouse IFN-γ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-103 96
EDTA plasma (n=8) 91-103 98
Cell culture media (n=8) 96-109 102

Linearity

Samples were spiked with high concentrations of Mouse IFN-γ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P01580
SynonymsIFNG, IFG, IFI, Type II Interferon
Research AreaCancer, Immunology, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. ADVANCED MATERIALS (2023) IF: 32.086
    Tumor Cell Nanovaccines based on Genetically Engineered Antibody-anchored Membrane

    DOI: 10.1002/adma.202208923

    PMID: 36715052

    		 Sample: bone-marrow-derived dendritic cells (BMDCs),Spleni
  2. Science Translational Medicine (2020) IF: 16.304
    Combination of metabolic intervention and T cell therapy enhances solid tumor immunotherapy

    DOI: 10.1126/scitranslmed.aaz6667

    PMID: 33239389

    		 Sample: Cell culture supernatant
  3. CHEMICAL ENGINEERING JOURNAL (2022) IF: 16.744
    Lyophilization-inactivated cancer cells composited Janus scaffold for tumor postoperative immuno-chemotherapy

    DOI: 10.1016/j.cej.2022.140619
    Sample: Bone marrow-derived dendritic cell (BMDC)
  4. CHEMICAL ENGINEERING JOURNAL (2022) IF: 16.744
    Enzyme-induced morphological transformation of self-assembled peptide nanovehicles potentiates intratumoral aggregation and inhibits tumour immunosuppression

    DOI: 10.1016/j.cej.2022.140466
    Sample: serum
  5. BIOMATERIALS (2022) IF: 15.304
    Self-amplified ROS production from fatty acid oxidation enhanced tumor immunotherapy by atorvastatin/PD-L1 siRNA lipopeptide nanoplexes

    DOI: 10.1016/j.biomaterials.2022.121902
    Sample: B16-F10 cell-xenograft,CT-26 cell-xenograft
  6. Acta Pharmaceutica Sinica B (2023) IF: 14.903
    Berberine improves central memory formation of CD8+ T cells: Implications for design of natural product-based vaccines

    DOI: 10.1016/j.apsb.2023.02.017
    Sample: Lymphocytes
  7. Science Advances (2022) IF: 14.957
    Glucocorticoid-induced loss of beneficial gut bacterial extracellular vesicles is associated with the pathogenesis of osteonecrosis

    DOI: 10.1126/sciadv.abg8335
    Sample: serum
  8. Journal for ImmunoTherapy of Cancer (2022) IF: 13.751
    CD40L-armed oncolytic herpes simplex virus suppresses pancreatic ductal adenocarcinoma by facilitating the tumor microenvironment favorable to cytotoxic T cell response in the syngeneic mouse model

    DOI: 10.1136/jitc-2021-003809

    PMID: 35086948

    		 Sample: Pancreatic ductal adenocarcinoma (PDAC) tumor tiss
  9. Journal for ImmunoTherapy of Cancer (2022) IF: 12.469
    Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis

    DOI: 10.1136/jitc-2021-004381

    PMID: 35738798

    		 Sample: B16F10 cell-xenograft
  10. ACS Applied Materials & Interfaces (2022) IF: 10.383
    Self-Activated Cascade Biocatalysis of Glucose Oxidase–Polycation–Iron Nanoconjugates Augments Cancer Immunotherapy

    DOI: 10.1021/acsami.2c04894
    Sample: serum
  11. ACS Applied Materials & Interfaces (2022) IF: 10.383
    Inflammation-Regulated Nanodrug Sensitizes Hepatocellular Carcinoma to Checkpoint Blockade Therapy by Reprogramming the Tumor Microenvironment

    DOI: 10.1021/acsami.2c14448

    PMID: 36314479

    		 Sample: Hepa 1-6 cells-xenograft
  12. JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 10.435
    Biomimetic nanoprobe-augmented triple therapy with photothermal, sonodynamic and checkpoint blockade inhibits tumor growth and metastasis

    DOI: 10.1186/s12951-022-01287-y

    PMID: 35168608

    		 Sample: tumor cell
  13. Nano-Micro Letters (2019) IF: 9.0429
    Inorganic Nanozyme with Combined Self-Oxygenation/Degradable Capabilities for Sensitized Cancer Immunochemotherapy

    DOI: 10.1007/s40820-019-0305-x
    Sample: Serum
  14. BRITISH JOURNAL OF PHARMACOLOGY (2023) IF: 9.4730
    Microsomal prostaglandin E synthase-1 inhibition prevents adverse cardiac remodeling after myocardial infarction in mice

    DOI: 10.1111/bph.16061
    Sample: Plasma
  15. JOURNAL OF PATHOLOGY (2023) IF: 9.8829
    MIF-inhibition alleviates vitiligo progression by suppressing CD8+T cell activation and proliferation

    DOI: 10.1002/path.6073
    Sample: serum
  16. OncoImmunology (2022) IF: 8.1099
    Carbon ion radiotherapy triggers immunogenic cell death and sensitizes melanoma to anti-PD-1 therapy in mice

    DOI: 10.1080/2162402X.2022.2057892

    PMID: 35355680

    		 Sample: serum
  17. JOURNAL OF INVESTIGATIVE DERMATOLOGY (2021) IF: 8.551
    Successful Treatment of Vitiligo with Cold Atmospheric Plasma‒Activated Hydrogel

    DOI: 10.1016/j.jid.2021.04.019

    PMID: 34029575

    		 Sample: Serum,Tissue homogenate
  18. Theranostics (2020) IF: 8.5790
    Engineering blood exosomes for tumor-targeting efficient gene/chemo combination therapy

    DOI: 10.7150/thno.45028

    PMID: 32685027

    		 Sample: Serum
  19. Frontiers in Immunology (2022) IF: 8.786
    Gut Microflora Modulates Th17/Treg Cell Differentiation in Experimental Autoimmune Prostatitis via the Short-Chain Fatty Acid Propionate

    DOI: 10.3389/fimmu.2022.915218

    PMID: 35860242

    		 Sample: serum
  20. CHEMOSPHERE (2022) IF: 8.943
    Oral administration of Bacillus cereus GW-01 alleviates the accumulation and detrimental effects of β-cypermethrin in mice

    DOI: 10.1016/j.chemosphere.2022.137333
    Sample: serum
  21. CHEMOSPHERE (2020) IF: 7.086
    Interleukin 17A deficiency alleviates fluoride-induced testicular injury by inhibiting the immune response and apoptosis

    DOI: 10.1016/j.chemosphere.2020.128178
    Sample: interstitial fluid
  22. Oxidative Medicine and Cellular Longevity (2023) IF: 7.31
    Combination Therapy with Indigo and Indirubin for Ulcerative Colitis via Reinforcing Intestinal Barrier Function

    DOI: 10.1155/2023/2894695
    Sample: colonic tissue
  23. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
    Study on a novel enzymatic colon-targeted particle of total saponins of Pulsatilla by mechanical grinding technology in a solvent free system

    DOI: 10.1016/j.biopha.2022.113645

    PMID: 36115109

    		 Sample: colon tissue
  24. FOOD CHEMISTRY (2021) IF: 7.514
    A new method to reduce allergenicity by improving the functional properties of soybean 7S protein through covalent modification with polyphenols

    DOI: 10.1016/j.foodchem.2021.131589
    Sample: Spleen cells
  25. Frontiers in Immunology (2021) IF: 7.561
    Pathogenic Roles of CXCL10 in Experimental Autoimmune Prostatitis by Modulating Macrophage Chemotaxis and Cytokine Secretion

    DOI: 10.3389/fimmu.2021.706027

    PMID: 34659199

    		 Sample: plasma,prostate tissue
  26. Frontiers in Immunology (2021) IF: 7.561
    IL-27 Mediates Pro-Inflammatory Effects via the ERK Signaling Pathway During Preterm Labor

    DOI: 10.3389/fimmu.2021.709229

    PMID: 34691022

    		 Sample: serum
  27. Frontiers in Immunology (2022) IF: 7.561
    HA/CD44 Regulates the T Helper 1 Cells Differentiation by Activating Annexin A1/Akt/mTOR Signaling to Drive the Pathogenesis of EAP.

    DOI: 10.3389/fimmu.2022.875412
    Sample: serum
  28. BIOLOGICAL PROCEDURES ONLINE (2022) IF: 7.717
    Angiotensin-converting enzyme 2 identifies immuno-hot tumors suggesting angiotensin-(1–7) as a sensitizer for chemotherapy and immunotherapy in breast cancer

    DOI: 10.1186/s12575-022-00177-9
    Sample: 4T1 cell-xenograft
  29. Frontiers in Microbiology (2022) IF: 6.064
    Recombinant Listeria ivanovii strain expressing listeriolysin O in place of ivanolysin O might be a potential antigen carrier for vaccine construction

    DOI: 10.3389/fmicb.2022.962326

    PMID: 35935244

    		 Sample: splenocytes
  30. Antioxidants (2021) IF: 6.313
    Aronia melanocarpa Extract Fermented by Lactobacillus plantarum EJ2014 Modulates Immune Response in Mice

    DOI: 10.3390/antiox10081276

    PMID: 34439524

    		 Sample: plasma
  31. Food & Function (2022) IF: 6.317
    Peanut allergen induces more serious allergic reactions than other allergens involving MAPK signaling pathways

    DOI: 10.1039/D2FO00777K

    PMID: 35920097

    		 Sample: serum
  32. International Journal of Nanomedicine (2021) IF: 6.4
    Chitosan-Poly(Acrylic Acid) Nanoparticles Loaded with R848 and MnCl2 Inhibit Melanoma via Regulating Macrophage Polarization and Dendritic Cell Maturation

    DOI: 10.2147/IJN.S318363
    Sample: serum,CD8+ T cell,BMDCs
  33. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2022) IF: 6.51
    Norcantharidin liposome emulsion hybrid delivery system enhances PD-1/PD-L1 immunotherapy by agonizing the non-canonical NF-κB pathway

    DOI: 10.1016/j.ijpharm.2022.122361
  34. Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
    Wheel-Running Exercise Protects Ovariectomized Mice from Bone Loss via IFN-γ-Mediated Suppression of the NF-κB and MAPK Pathways

    DOI: 10.1155/2022/2030818
    Sample: serum
  35. Frontiers in Nutrition (2022) IF: 6.59
    The Protective Effect of Sulforaphane on Dextran Sulfate Sodium-Induced Colitis Depends on Gut Microbial and Nrf2-Related Mechanism

    DOI: 10.3389/fnut.2022.893344
    Sample: Serum
  36. Oncotarget (2016) IF: 5.008
    Beauveria attenuates asthma by inhibiting inflammatory response and inducing lymphocytic cell apoptosis

    DOI: 10.18632/oncotarget.12958
    Sample: serum,lymphocytes
  37. Marine Drugs (2022) IF: 5.118
    Monkfish Peptides Mitigate High Fat Diet-Induced Hepatic Steatosis in Mice

    DOI: 10.3390/md20050312

    PMID: 35621963

    		 Sample: hepatic
  38. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020) IF: 5.162
    Structural characterization of a novel polysaccharide from Panax notoginseng residue and its immunomodulatory activity on bone marrow dendritic cells

    DOI: 10.1016/j.ijbiomac.2020.06.117

    PMID: 32553971

    		 Sample: Cell sample
  39. Oncotarget (2017) IF: 5.168
    Regulatory role of NKG2D+ NK cells in intestinal lamina propria by secreting double-edged Th1 cytokines in ulcerative colitis

    DOI: 10.18632/oncotarget.22132
    Sample: Tissue homogenate
  40. CELL CYCLE (2022) IF: 5.173
    Tanshinone increases Hemopexin expression in lung cells and macrophages to protect against cigarette smoke-induced COPD and enhance antiviral responses

    DOI: 10.1080/15384101.2022.2129933

    PMID: 36218263

    		 Sample: BALF,BEAS-2B cells
  41. JOURNAL OF ETHNOPHARMACOLOGY (2023) IF: 5.195
    Immunomodulatory effects and mechanisms of Tiepishihu Xiyangshen granules on cyclophosphamide induced immuno-suppression via TLR4/MAPKs and PI3K/AKT/FOXO3a signal pathways

    DOI: 10.1016/j.jep.2023.116192
    Sample: serum
  42. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2021) IF: 5.279
    Reducing the Allergenicity of α-Lactalbumin after Lipid Peroxidation

    DOI: 10.1021/acs.jafc.1c00559

    PMID: 33974424

    		 Sample: Cell culture supernatant
  43. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022) IF: 5.279
    Laminaria japonica Polysaccharide Suppresses Atherosclerosis via Regulating Autophagy-Mediated Macrophage Polarization

    DOI: 10.1021/acs.jafc.1c07483

    PMID: 35167294

    		 Sample: aortic,Raw264.7 cell
  44. PHYTOMEDICINE (2021) IF: 5.34
    Chrysin inhibits hepatocellular carcinoma progression through suppressing programmed death ligand 1 expression

    DOI: 10.1016/j.phymed.2021.153867

    PMID: 34923234

    		 Sample: H22 cell-xenograft
  45. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2018) IF: 5.5
    Anti-IFN-γ Antibody Promotes Osteoclastogenesis in Human Bone Marrow Monocyte-Derived Macrophages Co-Cultured with Tuberculosis-Activated Th1 Cells

    DOI: 10.1159/000493455
    Sample: CD4+T helper 1 cells(Th1 cells),bone marrow monocy
  46. Frontiers in Chemistry (2022) IF: 5.545
    Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency

    DOI: 10.3389/fchem.2022.955995

    PMID: 36034656

    		 Sample: 4T1 cell
  47. FREE RADICAL BIOLOGY AND MEDICINE (2019) IF: 5.657
    Oral administration of 3,3′-diselenodipropionic acid prevents thoracic radiation induced pneumonitis in mice by suppressing NF-kB/IL-17/G-CSF/neutrophil axis

    DOI: 10.1016/j.freeradbiomed.2019.09.009
    Sample: Bronchoalveolar Lavage Fluid,Tissue homogenate
  48. Nutrients (2021) IF: 5.719
    Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice

    DOI: 10.3390/nu13093209

    PMID: 34579088

    		 Sample: Splenocyte
  49. Clinical and Translational Allergy (2022) IF: 5.871
    MiR-29b regulates Th2 cell differentiation in asthma by targeting inducible B7-H3 and STAT3

    DOI: 10.1002/clt2.12114

    PMID: 35079347

    		 Sample: bronchoalveolar lavage fluid (BALF),Lung tissue
  50. Frontiers in Microbiology (2018) IF: 4.019
    The Influence of Acanthamoeba–Legionella Interaction in the Virulence of Two Different Legionella Species

    DOI: 10.3389/fmicb.2018.02962
    Sample: Cell culture supernatant
  51. AAPS PHARMSCITECH (2022) IF: 4.026
    Antitumor Immunotherapy of Sialic Acid and/or GM1 Modified Coenzyme Q10 Submicron Emulsion

    DOI: 10.1208/s12249-022-02426-2

    PMID: 36253573

    		 Sample: serum
  52. American Journal of Translational Research (2019) IF: 4.06
    Xiaoyaosan prevents atherosclerotic vulnerable plaque formation through heat shock protein/glucocorticoid receptor axis-mediated mechanism

    PMID: 31632527

    		 Sample: serum
  53. CARBOHYDRATE POLYMERS (2015) IF: 4.074
    Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis

    DOI: 10.1016/j.carbpol.2015.12.050
    Sample: Cell culture supernatant
  54. Frontiers in Microbiology (2017) IF: 4.076
    Oral Immunization with Recombinant Lactobacillus acidophilus Expressing espA-Tir-M Confers Protection against Enterohemorrhagic Escherichia coli O157:H7 Challenge in Mice

    DOI: 10.3389/fmicb.2017.00417
    Sample: Serum
  55. Foods (2020) IF: 4.092
    Protective Effect of Prunus mume Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis

    DOI: 10.3390/foods10010058

    PMID: 33383792

    		 Sample: colon tissue,serum
  56. INFLAMMATION (2022) IF: 4.092
    Pre-exposure to Aerosolized Polyvalent Bacterial Lysates Protects Against Bleomycin-Induced Pulmonary Fibrosis in Mice

    DOI: 10.1007/s10753-022-01653-w

    PMID: 35249190

    		 Sample: Bronchoalveolar lavage fluid (BALF)
  57. PROSTATE (2021) IF: 4.104
    Melatonin attenuates prostatic inflammation and pelvic pain via Sirt1-dependent inhibition of the NLRP3 inflammasome in an EAP mouse model

    DOI: 10.1002/pros.24214

    PMID: 34418127

    		 Sample: serum
  58. PROSTATE (2021) IF: 4.104
    4-Methylumbelliferone treatment and hyaluronan inhibition as a therapeutic strategy for chronic prostatitis

    DOI: 10.1002/pros.24205

    PMID: 34320251

    		 Sample: serum
  59. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2022) IF: 4.125
    Preventive and synbiotic effects of the soluble dietary fiber obtained from Lentinula edodes byproducts and Lactobacillus plantarum LP90 against dextran sulfate sodium-induced colitis in mice

    DOI: 10.1002/jsfa.12173

    PMID: 36054505

    		 Sample: serum,colon tissue
  60. Microbial Cell Factories (2020) IF: 4.187
    A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC

    DOI: 10.1186/s12934-020-01308-1

    PMID: 32075660

    		 Sample: Cell culture supernatant
  61. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2020) IF: 4.192
    Changes in Allergenicity of Ovalbumin in Vitro and in Vivo on Conjugation with Quercetin

    DOI: 10.1021/acs.jafc.0c00461

    PMID: 32182051

    		 Sample: Serum,Cell culture supernatant
  62. Frontiers in Microbiology (2020) IF: 4.236
    Immunization With a Secreted Esterase Protects Mice Against Multiple Serotypes (M1, M3, and M28) of Group A Streptococcus

    DOI: 10.3389/fmicb.2020.00565
    Sample: skin
  63. Scientific Reports (2017) IF: 4.259
    Rheb1 deletion in myeloid cells aggravates OVA-induced allergic inflammation in mice

    DOI: 10.1038/srep42655
    Sample: Bronchoalveolar Lavage Fluid,Serum
  64. Scientific Reports (2022) IF: 4.38
    Saffron extract and crocin exert anti-inflammatory and anti-oxidative effects in a repetitive mild traumatic brain injury mouse model

    DOI: 10.1038/s41598-022-09109-9

    PMID: 35322143

    		 Sample: brain
  65. COLLOIDS AND SURFACES B-BIOINTERFACES (2020) IF: 4.389
    Virus-like hollow mesoporous silica nanoparticles for cancer combination therapy

    DOI: 10.1016/j.colsurfb.2020.111452

    PMID: 33189035

    		 Sample: Serum
  66. JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION (2021) IF: 4.399
    Attenuation of virulence in multiple serotypes (M1, M3, and M28) of Group A Streptococcus after the loss of secreted esterase

    DOI: 10.1016/j.jmii.2021.09.008

    PMID: 34674958

    		 Sample: skin lesion
  67. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486
    Defibrotide inhibits donor leucocyte‐endothelial interactions and protects against acute graft‐versus‐host disease

    DOI: 10.1111/jcmm.15434
    Sample: Plasma
  68. Nutrients (2020) IF: 4.546
    Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function

    DOI: 10.3390/nu12010249

    PMID: 31963703

    		 Sample: Serum,Cell culture supernatant
  69. Frontiers in Genetics (2022) IF: 4.772
    Construction, bioinformatics analysis, and validation of competitive endogenous RNA networks in ulcerative colitis

    DOI: 10.3389/fgene.2022.951243
    Sample: Ulcerative colitis (UC) tissue
  70. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2020) IF: 4.845
    Targeted delivery of zoledronic acid through the sialic acid - Siglec axis for killing and reversal of M2 phenotypic tumor-associated macrophages – A promising cancer immunotherapy

    DOI: 10.1016/j.ijpharm.2020.119929
    Sample: Serum,Tissue homogenate
  71. Frontiers in Oncology (2020) IF: 4.848
    Cytotoxic T Cell Responses Induced by CS1/CRT Fusion DNA Vaccine in a Human Plasmacytoma Model

    DOI: 10.3389/fonc.2020.587237

    PMID: 33330069

    		 Sample: splenocytes
  72. MOLECULES (2022) IF: 4.927
    Ethyl Acetate Extract from Artemisia argyi Prevents Liver Damage in ConA-Induced Immunological Liver Injury Mice via Bax/Bcl-2 and TLR4/MyD88/NF-κB Signaling Pathways

    DOI: 10.3390/molecules27227883
    Sample: hepatic
  73. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    The immunomodulatory effects of ginsenoside derivative Rh2-O on splenic lymphocytes in H22 tumor-bearing mice is partially mediated by TLR4

    DOI: 10.1016/j.intimp.2021.108316

    PMID: 34768129

    		 Sample: splenic lymphocytes
  74. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Bixin protects mice against bronchial asthma though modulating PI3K/Akt pathway

    DOI: 10.1016/j.intimp.2021.108266

    PMID: 34678694

    		 Sample: BALF
  75. FASEB JOURNAL (2020) IF: 4.966
    CaMK4‐dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis

    DOI: 10.1096/fj.201902910RRR
    Sample: Cell culture supernatant,Tissue homogenate,serum
  76. Scientific Reports (2022) IF: 4.996
    Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis

    DOI: 10.1038/s41598-022-21816-x
    Sample: serum
  77. Bioengineered (2022) IF: 3.269
    Guizhi Fuling pill attenuates liver fibrosis in vitro and in vivo via inhibiting TGF-β1/Smad2/3 and activating IFN-γ/Smad7 signaling pathways

    DOI: 10.1080/21655979.2022.2054224

    PMID: 35387552

    		 Sample: liver tissue
  78. Food & Function (2018) IF: 3.289
    Structural characterization of two endopolysaccharides from Phellinus sp. and their immunologic effects by intragastric administration in a healthy mammalian model

    DOI: 10.1039/C7FO01161J
    Sample: serum
  79. VETERINARY MICROBIOLOGY (2021) IF: 3.293
    Molecular and functional characterization of HtrA protein in Actinobacillus pleuropneumoniae

    DOI: 10.1016/j.vetmic.2021.109058

    PMID: 33862332

    		 Sample: Serum
  80. INTERNATIONAL IMMUNOPHARMACOLOGY (2019) IF: 3.361
    Fusion of thymosin alpha 1 with mutant IgG1 CH3 prolongs half-life and enhances antitumor effects in vivo

    DOI: 10.1016/j.intimp.2019.05.047

    PMID: 31220695

    		 Sample: Serum
  81. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2018) IF: 3.388
    Aspergillus fumigatus Increased PAR-2 Expression and Elevated Proinflammatory Cytokines Expression Through the Pathway of PAR-2/ERK1/2 in Cornea

    DOI: 10.1167/iovs.17-21887

    PMID: 29332129

    		 Sample: Tissue homogenate
  82. CHEMICO-BIOLOGICAL INTERACTIONS (2019) IF: 3.407
    Pregnane X receptor activation by its natural ligand Ginkgolide-A improves tight junction proteins expression and attenuates bacterial translocation in cirrhosis

    DOI: 10.1016/j.cbi.2019.108891

    PMID: 31697926

    		 Sample: Plasma,Tissue homogenate
  83. PHARMACOLOGY (2023) IF: 3.429
    DPP4 Inhibitor Sitagliptin Reduces Inflammatory Responses and Mast Cell Activation in Allergic Rhinitis

    DOI: 10.1159/000528634
    Sample: serum，nasal lavage fluid
  84. ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY (2013) IF: 3.449
    Therapeutic effects of anti–B7-H3 antibody in an ovalbumin-induced mouse asthma model

    DOI: 10.1016/j.anai.2013.06.030
    Sample: Bronchoalveolar Lavage Fluid
  85. BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 3.457
    Effects of iron chelating agent on Schistosoma mansoni infected murine model

    DOI: 10.1016/j.biopha.2018.10.036

    PMID: 30391706

    		 Sample: Serum,Tissue homogenate
  86. Journal of Functional Foods (2018) IF: 3.47
    Components of heat-treated Helianthus annuus L. pectin inhibit tumor growth and promote immunity in a mouse CT26 tumor model

    DOI: 10.1016/j.jff.2018.07.001
    Sample: Tissue homogenate
  87. BMC IMMUNOLOGY (2022) IF: 3.594
    NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis

    DOI: 10.1186/s12865-022-00515-2

    PMID: 35965334

    		 Sample: Raw 264.7 cell
  88. GENE (2019) IF: 3.688
    The inhibition of microRNA-203 on ischemic reperfusion injury after total knee arthroplasty via suppressing MYD88-mdiated toll-like receptor signaling pathway

    DOI: 10.1016/j.gene.2019.02.030

    PMID: 30772517

    		 Sample: Tissue homogenate
  89. GENE (2016) IF: 3.688
    Immunogenicity of the recombinant HxuCBA proteins encoded by hxuCBA gene cluster of Haemophilus parasuis in mice

    DOI: 10.1016/j.gene.2016.07.001
    Sample: Serum
  90. Journal of Functional Foods (2020) IF: 3.701
    Comparison of the structural characteristics and immunostimulatory activities of polysaccharides from wild and cultivated Pleurotus feruleus

    DOI: 10.1016/j.jff.2020.104050
    Sample: serum
  91. MEDIATORS OF INFLAMMATION (2020) IF: 3.758
    I-alowCD11bhigh DC Regulates the Immune Response in the Eyes of Experimental Autoimmune Uveitis

    DOI: 10.1155/2020/6947482

    PMID: 32256194

    		 Sample: Serum,Cell culture supernatant,Aqueous Humor
  92. Heliyon (2022) IF: 3.776
    A sesquiterpene isolated from the stems and leaves of Dioscorea opposita thunb. Transforms the composition of immune cells through ERβ in a mouse model of LPS-induced lung injury

    DOI: 10.1016/j.heliyon.2022.e10500
    Sample: Serum,lung tissue
  93. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943
    Borneol inhibits CD4 + T cells proliferation by down-regulating miR-26a and miR-142-3p to attenuate asthma

    DOI: 10.1016/j.intimp.2020.107223
    Sample: Bronchoalveolar Lavage Fluid
  94. MICROBIAL PATHOGENESIS (2017) IF: 2.009
    Intranasal immunization with novel EspA-Tir-M fusion protein induces protective immunity against enterohemorrhagic Escherichia coli O157:H7 challenge in mice

    DOI: 10.1016/j.micpath.2017.01.062
    Sample: Serum
  95. PARASITOLOGY RESEARCH (2016) IF: 2.027
    spp. in experimentally infected immunocompetent and immunosuppresed mice

    DOI: 10.1007/s00436-016-4951-3
    Sample: Serum,Tissue homogenate
  96. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2015) IF: 2.297
    Ghrelin ameliorates intestinal barrier dysfunction in experimental colitis by inhibiting the activation of nuclear factor-kappa B

    DOI: 10.1016/j.bbrc.2015.01.083
    Sample: Tissue homogenate
  97. PARASITOLOGY RESEARCH (2022) IF: 2.383
    Plasmodium knowlesi Duffy binding protein alpha region II (PkDBPαII) in clinical isolates from Peninsular Malaysia and Malaysian Borneo exhibit different immune responses in animal models

    DOI: 10.1007/s00436-022-07665-7

    PMID: 36152079

    		 Sample: Spleen cell
  98. CHEMISTRY & BIODIVERSITY (2021) IF: 2.408
    Protective Effect of n-Butanol Extract from Viola yedoensis on Immunological Liver Injury

    DOI: 10.1002/cbdv.202001043

    PMID: 33929783

    		 Sample: Tissue homogenate(liver)
  99. VETERINARY MICROBIOLOGY (2018) IF: 2.525
    Dendritic cell-targeted recombinantLactobacilli induce DC activation and elicit specific immune responses against G57 genotype of avian H9N2 influenza virus infection

    DOI: 10.1016/j.vetmic.2018.07.009
    Sample: Serum,Cell culture supernatant
  100. MICROBIAL PATHOGENESIS (2019) IF: 2.581
    Metabolites of stable fly reduce diarrhea in mice by modulating the immune system, antioxidants, and composition of gut microbiota

    DOI: 10.1016/j.micpath.2019.103557
    Sample: serum,small intestinal tissues
  101. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
    Camelina sativa Oil Treatment Alleviates Castor Oil-Induced Diarrhea in ICR Mice by Regulating Intestinal Flora Composition

    DOI: 10.1155/2022/5394514

    PMID: 35178105

    		 Sample: plasma
  102. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
    Astragali Radix-Coptis Rhizoma Herb Pair Attenuates Atherosclerosis in ApoE-/- Mice by Regulating the M1/M2 and Th1/Th2 Immune Balance and Activating the STAT6 Signaling Pathway

    DOI: 10.1155/2022/7421265

    PMID: 35178108

    		 Sample: serum
  103. BMC Veterinary Research (2021) IF: 2.741
    The construction of recombinant Lactobacillus casei vaccine of PEDV and its immune responses in mice

    DOI: 10.1186/s12917-021-02885-y

    PMID: 33947419

    		 Sample: Serum
  104. PLoS One (2018) IF: 2.766
    A trivalent Apx-fusion protein delivered by E. coli outer membrane vesicles induce protection against Actinobacillus pleuropneumoniae of serotype 1 and 7 challenge in a murine model

    DOI: 10.1371/journal.pone.0191286
    Sample: serum
  105. MICROBIAL PATHOGENESIS (2020) IF: 2.914
    Protective immunity induced by a DNA vaccine cocktail expressing TgSAG1, TgROP2, and the genetic adjuvant HBsAg against Toxoplasma gondii infection

    DOI: 10.1016/j.micpath.2020.104441

    PMID: 32781100

    		 Sample: Serum,Cell culture supernatant
  106. MICROBIAL PATHOGENESIS (2020) IF: 2.914
    Curcumin ameliorates Staphylococcus aureus-induced mastitis injury through attenuating TLR2-mediated NF−κB activation

    DOI: 10.1016/j.micpath.2020.104054
    Sample: Serum
  107. INFLAMMATION (2019) IF: 2.939
    The Hypermethylation of Foxp3 Promoter Impairs the Function of Treg Cells in EAP

    DOI: 10.1007/s10753-019-01030-0

    PMID: 31209730

    		 Sample: Serum
  108. Molecular Medicine Reports (2019) IF: 2.952
    BCG stimulation promotes dendritic cell proliferation and expression of VDR and CYP27B1 in vitamin D‑deficient mice

    DOI: 10.3892/mmr.2019.10780
    Sample: BMDCs
  109. ONCOLOGY REPORTS (2018) IF: 2.976
    Effect of xanthohumol on Th1/Th2 balance in a breast cancer mouse model

    DOI: 10.3892/or.2017.6094
    Sample: serum
  110. JOURNAL OF VETERINARY MEDICAL SCIENCE (2022) IF: 1.105
    Immunogenicity and protection against Glaesserella parasuis serotype 13 infection after vaccination with recombinant protein LolA in mice

    DOI: 10.1292/jvms.22-0203

    PMID: 36216558

    		 Sample: serum
  111. NEUROREPORT (2018) IF: 1.266
    Role of receptor-interacting protein 1/receptor-interacting protein 3 in inflammation and necrosis following chronic constriction injury of the sciatic nerve

    DOI: 10.1097/WNR.0000000000001120

    PMID: 30192300

    		 Sample: Tissue homogenate
  112. JOURNAL OF VETERINARY MEDICAL SCIENCE (2021) IF: 1.267
    Application of mouse model for evaluation of recombinant LpxC and GmhA as novel antigenic vaccine candidates of Glaesserella parasuis serotype 13

    DOI: 10.1292/jvms.21-0298

    PMID: 34393140

    		 Sample: serum
  113. JOURNAL OF FOOD SAFETY (2018) IF: 1.275
    Staphylococcal enterotoxin M causes intestine dysfunction via activating inflammation

    DOI: 10.1111/jfs.12465
    Sample: plasma
  114. International Journal of Clinical and Experimental Medicine (2015) IF: 1.277
    Sulfated modification promotes the immunomodulatory bioactivities of Lyciumbarbarum polysaccharides in vitro

    PMID: 26884954

    		 Sample: lymphocyte
  115. ACTA PARASITOLOGICA (2021) IF: 1.44
    Evaluation of Allicin Against Alveolar Echinococcosis In Vitro and in a Mouse Model

    DOI: 10.1007/s11686-021-00434-z

    PMID: 34143400

    		 Sample: serum
  116. PARASITOLOGY RESEARCH (2020) IF: 1.641
    Effects of programmed cell death protein 10 on fecundity in Schistosoma japonicum

    DOI: 10.1007/s00436-020-06635-1
    Sample: serum
  117. RESEARCH IN VETERINARY SCIENCE (2019) IF: 1.751
    Amelioration effects of Kaempferol on immune response following chronic intermittent cold-stress

    DOI: 10.1016/j.rvsc.2019.08.012

    PMID: 31412308

    		 Sample: Serum,Tissue homogenate
  118. BIOMEDICAL AND ENVIRONMENTAL SCIENCES (2019) IF: 1.917
    Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice

    DOI: 10.3967/bes2018.101
    Sample: Plasma,Tissue homogenate
  119. Journal of Huazhong University of Science and Technology-Medical Sciences (2016) IF: 0.838
    Peroxisome proliferator-activated receptor-γ agonist pioglitazone fails to attenuate renal fibrosis caused by unilateral ureteral obstruction in mice

    DOI: 10.1007/s11596-016-1539-1
    Sample: Tissue homogenate
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