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Mouse IFN-γ(Interferon Gamma) ELISA Kit

  • Cat.No.:E-EL-M0048

  • Reactivity: Mouse

To Purchase E-EL-M0048

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IFN-γ in samples. No significant cross-reactivity or interference between Mouse IFN-γ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IFN-γ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFN-γ. You can calculate the concentration of Mouse IFN-γ in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IFN-γ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IFN-γ were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 51.50 96.50 497.20 53.30 95.50 533.30
Standard deviation 3.20 4.40 20.90 3.70 4.70 17.10
CV (%) 6.21 4.56 4.20 6.94 4.92 3.21

Recovery

The recovery of Mouse IFN-γ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-103 96
EDTA plasma (n=8) 91-103 98
Cell culture media (n=8) 96-109 102

Linearity

Samples were spiked with high concentrations of Mouse IFN-γ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P01580
SynonymsIFNG, IFG, IFI, Type II Interferon
Research AreaCancer,Immunology,Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Advanced materials (2019) IF: 27.398
    Dual-Locking Nanoparticles Disrupt the PD-1/PD-L1 Pathway for Efficient Cancer Immunotherapy

    DOI: 10.1002/adma.201905751

    PMID: 31709671

    Sample: Tissue homogenate
  2. Nano-Micro Letters (2019) IF: 12.264
    Inorganic Nanozyme with Combined Self-Oxygenation/Degradable Capabilities for Sensitized Cancer Immunochemotherapy

    DOI: 10.1007/s40820-019-0305-x

    PMID: 99999999

    Sample: Serum
  3. NANO LETTERS (2019) IF: 11.238
    NanoRNP Overcomes Tumor Heterogeneity in Cancer Treatment

    DOI: 10.1021/acs.nanolett.9b02501

    PMID: 31593471

    Sample: Serum
  4. Biomaterials (2020) IF: 10.317
    Virus-like nanoparticle as a co-delivery system to enhance efficacy of CRISPR/Cas9-based cancer immunotherapy

    DOI: 10.1016/j.biomaterials.2020.120275

    PMID: 99999999

    Sample: Serum,Cell lysate,Tissue homogenate
  5. Theranostics (2020) IF: 8.579
    Engineering blood exosomes for tumor-targeting efficient gene/chemo combination therapy

    DOI: 10.7150/thno.45028

    PMID: 32685027

    Sample: Serum
  6. Theranostics (2020) IF: 8.579
    Magnetic Targeted Near- Infrared II PA/MR Imaging-GuidedPhotothermal Therapy to Trigger Cancer Immunotherapy

    DOI: 10.7150/thno.43604

    PMID: 99999999

    Sample: Serum
  7. Free Radical Biology and Medicine (2019) IF: 6.17
    Oral administration of 3,3'-diselenodipropionic acid prevents thoracic radiation induced pneumonitis in mice by suppressing NF-kB/IL-17/G-CSF/neutrophil axis

    DOI: 10.1016/j.freeradbiomed.2019.09.009

    PMID: 31521664

    Sample: Bronchoalveolar Lavage Fluid,Tissue homogenate
  8. Oncotarget (2017) IF: 5.168
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    DOI: 10.18632/oncotarget.22132

    PMID: 29228739

    Sample: Tissue homogenate
  9. International Journal of Pharmaceutics (2020) IF: 4.845
    Targeted delivery of zoledronic acid through the sialic acid - Siglec axis for killing and reversal of M2 phenotypic tumor-associated macrophages - A promising cancer immunotherapy

    DOI: 10.1016/j.ijpharm.2020.119929

    PMID: 33010395

    Sample: Serum,Tissue homogenate
  10. Journal of Cellular and Molecular Medicine (2020) IF: 4.486
    Defibrotide inhibits donor leucocyte-endothelial interactions and protects against acute graft-versus-host disease

    DOI: 10.1111/jcmm.15434

    PMID: 32519822

    Sample: Plasma
  11. Journal of Agricultural and Food Chemistry (2020) IF: 4.192
    Changes in Allergenicity of Ovalbumin in Vitro and in Vivo on Conjugation with Quercetin

    DOI: 10.1021/acs.jafc.0c00461

    PMID: 32182051

    Sample: Serum,Cell culture supernatant
  12. Microbial Cell Factories (2020) IF: 4.187
    A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC

    DOI: 10.1186/s12934-020-01308-1

    PMID: 32075660

    Sample: Cell culture supernatant
  13. Nutrients (2020) IF: 4.546
    Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function

    DOI: 10.3390/nu12010249

    PMID: 31963703

    Sample: Serum,Cell culture supernatant
  14. Frontiers in microbiology (2018) IF: 4.019
    The influence of Acanthamoeba-Legionella interaction in the virulence of two different Legionella species.

    DOI: 10.3389/fmicb.2018.02962

    PMID: 30568639

    Sample: Cell culture supernatant
  15. Scientific Reports (2017) IF: 4.259
    Rheb1 Deletion In Myeloid Cells Aggravates OVA-Induced Allergic Inflammation In Mice.

    DOI: 10.1038/srep42655

    PMID: 28225024

    Sample: Bronchoalveolar Lavage Fluid,Serum
  16. Frontiers in Microbiology (2017) IF: 4.076
    Oral Immunization With Recombinant Lactobacillus Acidophilus Expressing espA-Tir-M Confers Protection Against Enterohemorrhagic Escherichia Coli O157: H7 Challenge In Mice.

    DOI: 10.3389/fmicb.2017.00417

    PMID: 28360900

    Sample: Serum
  17. Carbohydrate Polymers (2016) IF: 4.811
    Structural Analysis And Immunoregulation Activity Comparison Of Five Polysaccharides From Angelica Sinensis.

    DOI: 10.1016/j.carbpol.2015.12.050

    PMID: 26876821

    Sample: Cell culture supernatant
  18. Mediators of Inflammation (2020) IF: 3.758
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    DOI: 10.1155/2020/6947482

    PMID: 32256194

    Sample: Serum,Cell culture supernatant,Aqueous Humor
  19. Chemico-Biological Interactions (2020) IF: 3.723
    Pregnane X receptor activation by its natural ligand Ginkgolide-A improves tight junction proteins expression and attenuates bacterial translocation in cirrhosis

    DOI: 10.1016/j.cbi.2019.108891

    PMID: 31697926

    Sample: Plasma,Tissue homogenate
  20. American Journal of Translational Research (2019) IF: 3.375
    Xiaoyaosan prevents atherosclerotic vulnerable plaque formation through heat shock protein/glucocorticoid receptor axis-mediated mechanism

    DOI: 99999999

    PMID: 31632527

    Sample: Serum
  21. Inflammation (2019) IF: 3.212
    The Hypermethylation of Foxp3 Promoter Impairs the Function of Treg Cells in EAP

    DOI: 10.1007/s10753-019-01030-0

    PMID: 31209730

    Sample: Serum
  22. Investigative ophthalmology & visual science (2018) IF: 3.27
    Aspergillus fumigatus Increased PAR-2 Expression and Elevated Proinflammatory Cytokines Expression Through the Pathway of PAR-2/ERK1/2 in Cornea

    DOI: 10.1167/iovs.17-21887

    PMID: 29332129

    Sample: Tissue homogenate
  23. Biomedicine & Pharmacotherapy (2019) IF: 3.457
    Effects of iron chelating agent on Schistosoma mansoni infected murine model

    DOI: 10.1016/j.biopha.2018.10.036

    PMID: 30391706

    Sample: Serum,Tissue homogenate
  24. IF: 3.361
    Fusion of thymosin alpha 1 with mutant IgG1 CH3 prolongs half-life and enhances antitumor effects in vivo
    Sample: Serum
  25. International Immunopharmacology (2019) IF: 3.361
    Fusion of thymosin alpha 1 with mutant IgG1 CH3 prolongs half-life and enhances antitumor effects in vivo

    DOI: 10.1016/j.intimp.2019.05.047

    PMID: 31220695

    Sample: Serum
  26. Journal of Functional Foods (2018) IF: 3.47
    Components of heat-treated Helianthus annuus L. pectin inhibit tumor growth and promote immunity in a mouse CT26 tumor model.

    DOI: 10.1016/j.jff.2018.07.001

    Sample: Tissue homogenate
  27. Annals of Allergy Asthma & Immunology (2013) IF: 3.728
    Therapeutic Effects Of Anti-B7-H3 Antibody In An Ovalbumin-Induced Mouse Asthma Model.

    DOI: 10.1016/j.anai.2013.06.030

    PMID: 24054363

    Sample: Bronchoalveolar Lavage Fluid
  28. Microbial Pathogenesis (2020) IF: 2.914
    Protective immunity induced by a DNA vaccine cocktail expressing TgSAG1, TgROP2, and the genetic adjuvant HBsAg against Toxoplasma gondii infection

    DOI: 10.1016/j.micpath.2020.104441

    PMID: 32781100

    Sample: Serum,Cell culture supernatant
  29. Microbial Pathogenesis (2020) IF: 2.914
    Curcumin ameliorates Staphylococcus aureus-induced mastitis injury through attenuating TLR2-mediated NF-κB activation

    DOI: 10.1016/j.micpath.2020.104054

    PMID: 32061917

    Sample: Serum
  30. Gene (2019) IF: 2.498
    The inhibition of microRNA-203 on ischemic reperfusion injury after total knee arthroplasty via suppressing MYD88-mdiated toll-like receptor signaling pathway

    DOI: 10.1016/j.gene.2019.02.030

    PMID: 30772517

    Sample: Tissue homogenate
  31. Biomedical and Environmental Sciences (2018) IF: 2.518
    Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice.

    DOI: 10.3967/bes2018.101

    PMID: 30423277

    Sample: Plasma,Tissue homogenate
  32. Veterinary Microbiology (2018) IF: 2.524
    Dendritic cell-targeted recombinant Lactobacilli induce DC activation and elicit specific immune responses against G57 genotype of avian H9N2 influenza virus infection.

    DOI: 10.1016/j.vetmic.2018.07.009

    PMID: 30173758

    Sample: Serum,Cell culture supernatant
  33. Microbial Pathogenesis (2017) IF: 2.009
    Intranasal Immunization With Novel EspA-Tir-M Fusion Protein Induces Protective Immunity Against Enterohemorrhagic Escherichia coli O157: H7 Challenge In Mice.

    DOI: 10.1016/j.micpath.2017.01.062

    PMID: 28163157

    Sample: Serum
  34. Gene (2016) IF: 2.415
    Immunogenicity Of The Recombinant HxuCBA Proteins Encoded By HxuCBA Gene Cluster Of Haemophilus Parasuis In Mice.

    DOI: 10.1016/j.gene.2016.07.001

    PMID: 27378742

    Sample: Serum
  35. Parasitology Research (2016) IF: 2.329
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    DOI: 10.1007/s00436-016-4951-3

    PMID: 26860840

    Sample: Serum,Tissue homogenate
  36. Biochemical & Biophysical Research Communications (2015) IF: 2.466
    Ghrelin Ameliorates Intestinal Barrier Dysfunction In Experimental Colitis By Inhibiting The Activation Of Nuclear Factor-Kappa B.

    DOI: 10.1016/j.bbrc.2015.01.083

    PMID: 25634696

    Sample: Tissue homogenate
  37. Research in veterinary science (2019) IF: 1.892
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    DOI: 10.1016/j.rvsc.2019.08.012

    PMID: 31412308

    Sample: Serum,Tissue homogenate
  38. NeuroReport (2018) IF: 1.266
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    DOI: 10.1097/WNR.0000000000001120

    PMID: 30192300

    Sample: Tissue homogenate
  39. Experimental Lung Research (2016) IF: 1.394
    Treatment Of Allergic Rhinitis With CpG Oligodeoxynucleotides Alleviates The Lower Airway Outcomes Of Combined Allergic Rhinitis And Asthma Syndrome Via A Mechanism That Possibly Involves In TSLP.

    DOI: 10.1080/01902148.2016.1215571

    PMID: 27541375

    Sample: Bronchoalveolar Lavage Fluid
  40. International Journal of Clinical and Experimental Medicine (2015) IF: 1.069
    Sulfated Modification Promotes The Immunomodulatory Bioactivities Of Lyciumbarbarum Polysaccharides In Vitro.

    PMID: 26884954

    Sample: Cell culture supernatant
  41. Indian Journal of Surgery (2020) IF: 0.561
    Structural characterization of a novel polysaccharide from Panax notoginseng residue and its immunomodulatory activity on bone marrow dendritic cells

    DOI: 10.1016/j.ijbiomac.2020.06.117

    PMID: 32553971

    Sample: Cell sample
  42. The FASEB Journal (2020)
    CaMK4-dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis

    DOI: 10.1096/fj.201902910RRR

    PMID: 32862457

    Sample: Cell culture supernatant,Tissue homogenate
  43. Journal of Huazhong University Of Science And Technology-Medical Sciences (2016) IF: 0.964
    Peroxisome Proliferator-Activated Receptor-Γ Agonist Pioglitazone Fails To Attenuate Renal Fibrosis Caused By Unilateral Ureteral Obstruction In Mice.

    DOI: 10.1007/s11596-016-1539-1

    PMID: 26838738

    Sample: Tissue homogenate
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