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Mouse IL-10(Interleukin 10) ELISA Kit

Citations(53)Uniprot : P18893

  • Cat.No.:E-EL-M0046

  • Reactivity: Mouse
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IL-10 in samples. No significant cross-reactivity or interference between Mouse IL-10 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IL-10 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-10. You can calculate the concentration of Mouse IL-10 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-10 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-10 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 51.00 125.60 414.80 53.20 121.40 406.80
Standard deviation 2.70 6.00 12.40 3.60 6.20 18.70
CV (%) 5.29 4.78 2.99 6.77 5.11 4.60

Recovery

The recovery of Mouse IL-10 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-108 102
EDTA plasma (n=8) 88-102 94
Cell culture media (n=8) 86-97 92

Linearity

Samples were spiked with high concentrations of Mouse IL-10 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P18893
SynonymsIL10, IL10A, CSIF, TGIF
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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  1. Biomaterials (2020) IF: 10.317
    Virus-like nanoparticle as a co-delivery system to enhance efficacy of CRISPR/Cas9-based cancer immunotherapy

    DOI: 10.1016/j.biomaterials.2020.120275
    Sample: Serum,Cell lysate,Tissue homogenate
  2. Small (2019) IF: 9.5980
    Neoadjuvant chemotherapy based on abraxane/human neutrophils cytopharmaceuticals with radiotherapy for gastric cancer

    DOI: 10.1002/smll.201804191

    PMID: 30549431

    		 Sample: Serum,Tissue homogenate
  3. Free Radical Biology and Medicine (2019) IF: 6.17
    Oral administration of 3,3'-diselenodipropionic acid prevents thoracic radiation induced pneumonitis in mice by suppressing NF-kB/IL-17/G-CSF/neutrophil axis

    DOI: 10.1016/j.freeradbiomed.2019.09.009

    PMID: 31521664

    		 Sample: Bronchoalveolar Lavage Fluid,Tissue homogenate
  4. Oxidative medicine and cellular longevity (2019) IF: 5.076
    Targeting IL-17A Improves the Dysmotility of the Small Intestine and Alleviates the Injury of the Interstitial Cells of Cajal during Sepsis

    DOI: 10.1155/2019/1475729

    PMID: 31531179

    		 Sample: Tissue homogenate
  5. Oncotarget (2017) IF: 5.168
    Regulatory role of NKG2D+ NK cells in intestinal lamina propria by secreting double-edged Th1 cytokines in ulcerative colitis.

    DOI: 10.18632/oncotarget.22132

    PMID: 29228739

    		 Sample: Tissue homogenate
  6. International Journal of Pharmaceutics (2020) IF: 4.845
    Targeted delivery of zoledronic acid through the sialic acid - Siglec axis for killing and reversal of M2 phenotypic tumor-associated macrophages - A promising cancer immunotherapy

    DOI: 10.1016/j.ijpharm.2020.119929

    PMID: 33010395

    		 Sample: Serum,Tissue homogenate
  7. Food & Function (2020) IF: 4.171
    Melatonin as an inducer of arecoline and their coordinated roles in anti-oxidative activity and immune responses

    DOI: 10.1039/d0fo01841d

    PMID: 32955540

    		 Sample: Plasma
  8. Food & Function (2020) IF: 4.171
    Flaxseed oligosaccharides alleviate DSS-induced colitis through modulation of gut microbiota and repair of the intestinal barrier in mice

    DOI: 10.1039/d0fo01105c

    PMID: 32856645

    		 Sample: Serum,Tissue homogenate
  9. Journal of Cellular and Molecular Medicine (2020) IF: 4.486
    Defibrotide inhibits donor leucocyte-endothelial interactions and protects against acute graft-versus-host disease

    DOI: 10.1111/jcmm.15434

    PMID: 32519822

    		 Sample: Plasma
  10. ACS Chemical Neuroscience (2020) IF: 4.486
    MG53 Protects hUC-MSCs against Inflammatory Damage and Synergistically Enhances Their Efficacy in Neuroinflammation Injured Brain through Inhibiting NLRP3/Caspase-1/IL-1β Axis

    DOI: 10.1021/acschemneuro.0c00268

    PMID: 32786312

    		 Sample: Serum
  11. international journal of pharmaceutics (2020) IF: 4.845
    Targeted delivery of zoledronic acid thrpugh the sialic acid siglec axis for killing and reversal of M2 phenotypic tumor-associated macrophages-A promising cancer immunotherapy

    DOI: http://doi.org/10.1016/j.ijpharm.2020.119929
    Sample: Serum,Cell lysate,Tissue homogenate
  12. Ecotoxicology and Environmental Safety (2020) IF: 4.872
    Silica particles disorganize the polarization of pulmonary macrophages in mice

    DOI: 10.1016/j.ecoenv.2020.110364

    PMID: 32114243

    		 Sample: Tissue homogenate
  13. Journal of Agricultural and Food Chemistry (2020) IF: 4.192
    Modulation of Gut Microbiota by Fucoxanthin During Alleviation of Obesity in High-Fat Diet-Fed Mice

    DOI: 10.1021/acs.jafc.0c01467

    PMID: 32309947

    		 Sample: Serum,Tissue homogenate
  14. Microbial Cell Factories (2020) IF: 4.187
    A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC

    DOI: 10.1186/s12934-020-01308-1

    PMID: 32075660

    		 Sample: Cell culture supernatant
  15. Ecotoxicology and Environmental Safety (2020) IF: 4.872
    Fine particulate matter increases airway hyperresponsiveness through kallikrein-bradykinin pathway

    DOI: 10.1016/j.ecoenv.2020.110491

    PMID: 32213367

    		 Sample: Cell culture supernatant
  16. International journal of molecular sciences (2019) IF: 4.556
    Resveratrol Plays a Protective Role against Premature Ovarian Failure and Prompts Female Germline Stem Cell Survival

    DOI: 10.3390/ijms20143605

    PMID: 31340581

    		 Sample: Serum,Cell culture supernatant
  17. Oxidative Medicine And Cellular Longevity (2016) IF: 4.593
    Hydrogen Sulfide Mitigates Kidney Injury In High Fat Diet-Induced Obese Mice.

    DOI: 10.1155/2016/2715718

    PMID: 27413418

    		 Sample: Plasma
  18. Frontiers in Microbiology (2017) IF: 4.076
    Oral Immunization With Recombinant Lactobacillus Acidophilus Expressing espA-Tir-M Confers Protection Against Enterohemorrhagic Escherichia Coli O157: H7 Challenge In Mice.

    DOI: 10.3389/fmicb.2017.00417

    PMID: 28360900

    		 Sample: Serum
  19. Life Sciences (2020) IF: 3.647
    Sevoflurane up-regulates microRNA-204 to ameliorate myocardial ischemia/reperfusion injury in mice by suppressing Cotl1

    DOI: 10.1016/j.lfs.2020.118162

    PMID: 32730836

    		 Sample: Tissue homogenate
  20. International Immunopharmacology (2020) IF: 3.943
    Myricitrin pretreatment ameliorates mouse liver ischemia reperfusion injury

    DOI: 10.1016/j.intimp.2020.107005

    PMID: 33045574

    		 Sample: Serum
  21. Experimental Biology and Medicine (2020) IF: 3.139
    Exosomes derived from synovial fibroblasts under hypoxia aggravate rheumatoid arthritis by regulating Treg/Th17 balance

    DOI: 10.1177/1535370220934736

    PMID: 32615822

    		 Sample: Cell culture medium
  22. Mediators of Inflammation (2020) IF: 3.758
    I-alowCD11bhigh DC Regulates the Immune Response in the Eyes of Experimental Autoimmune Uveitis

    DOI: 10.1155/2020/6947482

    PMID: 32256194

    		 Sample: Serum,Cell culture supernatant,Aqueous Humor
  23. International Immunopharmacology (2020) IF: 3.943
    Molecular hydrogen attenuates sepsis-induced neuroinflammation through regulation of microglia polarization through an mTOR-autophagy-dependent pathway

    DOI: 10.1016/j.intimp.2020.106287

    PMID: 32058932

    		 Sample: Tissue homogenate
  24. Inflammation (2019) IF: 3.212
    The Hypermethylation of Foxp3 Promoter Impairs the Function of Treg Cells in EAP

    DOI: 10.1007/s10753-019-01030-0

    PMID: 31209730

    		 Sample: Serum
  25. Biomedicine & Pharmacotherapy (2019) IF: 3.457
    Effects of iron chelating agent on Schistosoma mansoni infected murine model

    DOI: 10.1016/j.biopha.2018.10.036

    PMID: 30391706

    		 Sample: Serum,Tissue homogenate
  26. Life sciences (2019) IF: 3.234
    GPR 30 reduces myocardial infarct area and fibrosis in female ovariectomized mice by activating the PI3K/AKT pathway

    DOI: 10.1016/j.lfs.2019.03.049

    PMID: 30905784

    		 Sample: Serum
  27. Neural plasticity (2019) IF: 3.161
    HMG-CoA Reductase Inhibitors Attenuate Neuronal Damage by Suppressing Oxygen Glucose Deprivation-Induced Activated Microglial Cells

    DOI: 10.1155/2019/7675496

    PMID: 30911291

    		 Sample: Cell culture supernatant
  28. Journal of Functional Foods (2019) IF: 3.47
    The protective effects of Chinese yam polysaccharide against obesity-induced insulin resistance

    DOI: 10.1016/j.jff.2019.02.023
    Sample: Serum
  29. World Journal of Gastroenterology (2017) IF: 3.365
    Anti-Oxidant And Anti-inflammatory Effects Of Hydrogen-Rich Water Alleviate Ethanol-Induced Fatty Liver In Mice.

    DOI: 10.3748/wjg.v23.i27.4920

    PMID: 28785146

    		 Sample: Serum
  30. Plants (2020) IF: 2.762
    Phytochemical, Cytotoxicity, Antioxidant and Anti-Inflammatory Effects of Psilocybe Natalensis Magic Mushroom

    DOI: 10.3390/plants9091127

    PMID: 32878164

    		 Sample: Cell culture supernatant
  31. Journal of Neuroscience Methods (2020) IF: 2.214
    Frequent exposure to varied home cage sizes alters pain sensitivity and some key inflammation-related biomarkers

    DOI: 10.1016/j.jneumeth.2020.108890

    PMID: 32768413

    		 Sample: Tissue homogenate
  32. Brain and behavior (2020) IF: 2.091
    MicroRNA-345-5p regulates depression by targeting suppressor of cytokine signaling 1

    DOI: 10.1002/brb3.1653

    PMID: 32730696

    		 Sample: Cell culture supernatant
  33. Dermatologic Therapy (2020) IF: 2.327
    NB‐UVB irradiation attenuates inflammatory response in psoriasis

    DOI: 10.1111/dth.13626

    PMID: 32431049

    		 Sample: Serum
  34. Microbial Pathogenesis (2020) IF: 2.914
    Exposure time determines the protective effect of Trichinella spiralis on experimental colitis

    DOI: 10.1016/j.micpath.2020.104263

    PMID: 32442663

    		 Sample: Serum
  35. Microbial Pathogenesis (2020) IF: 2.914
    Curcumin ameliorates Staphylococcus aureus-induced mastitis injury through attenuating TLR2-mediated NF-κB activation

    DOI: 10.1016/j.micpath.2020.104054

    PMID: 32061917

    		 Sample: Serum
  36. PeerJ (2020) IF: 2.379
    The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis

    DOI: 10.7717/peerj.8294

    PMID: 32030319

    		 Sample: Tissue homogenate
  37. Medicine (2018) IF: 2.028
    Effect of gap junctions on RAW264. 7 macrophages infected with H37Rv

    DOI: 10.1097/MD.0000000000012125

    PMID: 30170447

    		 Sample: Cell culture supernatant
  38. Bioscience reports (2018) IF: 2.899
    Maggot protein ameliorates dextran sulphate sodium-induced ulcerative colitis in mice

    DOI: 10.1042/BSR20181799

    PMID: 30393231

    		 Sample: Serum
  39. Biochemical and biophysical research communications (2019) IF: 2.559
    Eosinophils attenuate arthritis by inducing M2 macrophage polarization via inhibiting the IkB/P38 MAPK signaling pathway

    DOI: 10.1016/j.bbrc.2018.12.010

    PMID: 30528734

    		 Sample: Tissue homogenate,Cell culture supernatant
  40. Gene (2019) IF: 2.498
    The inhibition of microRNA-203 on ischemic reperfusion injury after total knee arthroplasty via suppressing MYD88-mdiated toll-like receptor signaling pathway

    DOI: 10.1016/j.gene.2019.02.030

    PMID: 30772517

    		 Sample: Tissue homogenate
  41. Microbial Pathogenesis (2017) IF: 2.009
    Intranasal Immunization With Novel EspA-Tir-M Fusion Protein Induces Protective Immunity Against Enterohemorrhagic Escherichia coli O157: H7 Challenge In Mice.

    DOI: 10.1016/j.micpath.2017.01.062

    PMID: 28163157

    		 Sample: Serum
  42. International Immunopharmacology (2016) IF: 2.956
    MiR-146a Modulates Macrophage Polarization By Inhibiting Notch1 Pathway In RAW264. 7 Macrophages.

    DOI: 10.1016/j.intimp.2016.01.009

    PMID: 26800097

    		 Sample: Cell culture supernatant
  43. Molecules (2017) IF: 2.861
    Capparis Spinosa Fruit Ethanol Extracts Exert Different Effects On The Maturation Of Dendritic Cells.

    DOI: 10.3390/molecules22010097

    PMID: 28067853

    		 Sample: Cell culture supernatant
  44. Parasitology Research (2016) IF: 2.329
    Immunopathological Assessments Of Human Blastocystis Spp. In Experimentally Infected Immunocompetent And Immunosuppresed Mice.

    DOI: 10.1007/s00436-016-4951-3

    PMID: 26860840

    		 Sample: Serum,Tissue homogenate
  45. Biochemical & Biophysical Research Communications (2015) IF: 2.466
    Ghrelin Ameliorates Intestinal Barrier Dysfunction In Experimental Colitis By Inhibiting The Activation Of Nuclear Factor-Kappa B.

    DOI: 10.1016/j.bbrc.2015.01.083

    PMID: 25634696

    		 Sample: Tissue homogenate
  46. Journal of Virological Methods (2020) IF: 1.786
    Colonisation of mice and pigs by a chimeric porcine circovirus 1-2 prototype vaccine strain and a PCV2 isolate originating in China and their induction of cytokines

    DOI: 10.1016/j.jviromet.2020.113905

    PMID: 32502500

    		 Sample: Cell culture supernatant
  47. Experimental and Therapeutic Medicine (2020) IF: 1.785
    Clostridium butyricum protects intestinal barrier function via upregulation of tight junction proteins and activation of the Akt/mTOR signaling pathway in a mouse model of dextran sodium sulfate-induced colitis

    DOI: 10.3892/etm.2020.9138

    PMID: 32934675

    		 Sample: Serum
  48. Journal of Food Biochemistry (2019) IF: 1.662
    Immunomodulatory effect of papaya (Carica papaya) pulp and seed extracts as a potential natural treatment for bacterial stress

    DOI: 10.1111/jfbc.13050

    PMID: 31571245

    		 Sample: Serum
  49. Aesthetic plastic surgery (2019) IF: 1.484
    M1/M2 Macrophages Play Different Roles in Adipogenic Differentiation of PDGFRα+ Preadipocytes In Vitro

    DOI: 10.1007/s00266-018-1294-8

    PMID: 30552469

    		 Sample: Cell culture supernatant
  50. The FASEB Journal (2020)
    CaMK4-dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis

    DOI: 10.1096/fj.201902910RRR

    PMID: 32862457

    		 Sample: Cell culture supernatant,Tissue homogenate
  51. bioRxiv (2020)
    Fecal microbiota transplantation reconstructs the gut microbiota of septic mice and protects the intestinal mucosal barrier

    DOI: 10.1101/2020.06.22.164541
    Sample: Serum
  52. Functional Foods in Health and Disease (2018)
    Immunological effects of Kefir produced from Kefir grains versus starter cultures when fed to mice

    DOI: 10.31989/ffhd.v8i8.533
    Sample: Tissue homogenate
  53. Journal of Huazhong University Of Science And Technology-Medical Sciences (2016) IF: 0.964
    Peroxisome Proliferator-Activated Receptor-Γ Agonist Pioglitazone Fails To Attenuate Renal Fibrosis Caused By Unilateral Ureteral Obstruction In Mice.

    DOI: 10.1007/s11596-016-1539-1

    PMID: 26838738

    		 Sample: Tissue homogenate
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