Click here to view cell apoptosis assay products
×

Mouse IL-17A(Interleukin 17A) ELISA Kit

  • Cat.No.:E-EL-M0047

  • Reactivity: Mouse

To Purchase E-EL-M0047

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IL-17A in samples. No significant cross-reactivity or interference between Mouse IL-17A and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IL-17A concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-17A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-17A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-17A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-17A. You can calculate the concentration of Mouse IL-17A in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-17A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-17A were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 47.50 108.60 431.30 46.10 109.50 437.90
Standard deviation 2.80 5.40 22.90 2.30 5.00 16.20
CV (%) 5.89 4.97 5.31 4.99 4.57 3.70

Recovery

The recovery of Mouse IL-17A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-107 102
EDTA plasma (n=8) 91-106 97
Cell culture media (n=8) 94-105 100

Linearity

Samples were spiked with high concentrations of Mouse IL-17A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q62386
SynonymsCTLA8, CTLA-8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL17, IL-17, IL17A, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. npj Regenerative Medicine (2022) IF: 14.404
    Enforced mesenchymal stem cell tissue colonization counteracts immunopathology

    DOI: 10.1038/s41536-022-00258-z

    PMID: 36261464

    Sample: plasma,AdMSCs
  2. Cell Death & Disease (2020) IF: 8.4689
    MicroRNA-377-3p released by mesenchymal stem cell exosomes ameliorates lipopolysaccharide-induced acute lung injury by targeting RPTOR to induce autophagy

    DOI: 10.1038/s41419-020-02857-4

    Sample: BALF
  3. JOURNAL OF INVESTIGATIVE DERMATOLOGY (2021) IF: 8.551
    Successful Treatment of Vitiligo with Cold Atmospheric Plasma‒Activated Hydrogel

    DOI: 10.1016/j.jid.2021.04.019

    PMID: 34029575

    Sample: Serum,Tissue homogenate
  4. Frontiers in Immunology (2022) IF: 8.786
    Gut Microflora Modulates Th17/Treg Cell Differentiation in Experimental Autoimmune Prostatitis via the Short-Chain Fatty Acid Propionate

    DOI: 10.3389/fimmu.2022.915218

    PMID: 35860242

    Sample: serum,prostate tissue
  5. CHEMOSPHERE (2020) IF: 7.086
    Interleukin 17A deficiency alleviates fluoride-induced testicular injury by inhibiting the immune response and apoptosis

    DOI: 10.1016/j.chemosphere.2020.128178

    Sample: interstitial fluid
  6. Oxidative Medicine and Cellular Longevity (2023) IF: 7.31
    Combination Therapy with Indigo and Indirubin for Ulcerative Colitis via Reinforcing Intestinal Barrier Function

    DOI: 10.1155/2023/2894695

    Sample: colonic tissue
  7. Frontiers in Immunology (2022) IF: 7.561
    HA/CD44 Regulates the T Helper 1 Cells Differentiation by Activating Annexin A1/Akt/mTOR Signaling to Drive the Pathogenesis of EAP.

    DOI: 10.3389/fimmu.2022.875412

    Sample: serum
  8. ACTA PHARMACOLOGICA SINICA (2021) IF: 6.15
    Interleukin-17 upregulation participates in the pathogenesis of heart failure in mice via NF-κB-dependent suppression of SERCA2a and Cav1.2 expression

    DOI: 10.1038/s41401-020-00580-6

    PMID: 33589793

    Sample: Plasma,Tissue homogenate(heart)
  9. Oncotarget (2017) IF: 5.168
    Regulatory role of NKG2D+ NK cells in intestinal lamina propria by secreting double-edged Th1 cytokines in ulcerative colitis

    DOI: 10.18632/oncotarget.22132

    Sample: Tissue homogenate
  10. NEUROPHARMACOLOGY (2022) IF: 5.251
    PEA-OXA ameliorates allodynia, neuropsychiatric and adipose tissue remodeling induced by social isolation

    DOI: 10.1016/j.neuropharm.2022.108978

    PMID: 35157898

    Sample: Mesenchymal stromal cells (MSCs)
  11. Frontiers in Cellular and Infection Microbiology (2021) IF: 5.293
    Appetite Suppression and Interleukin 17 Receptor Signaling Activation of Colonic Mycobiota Dysbiosis Induced by High Temperature and High Humidity Conditions

    DOI: 10.3389/fcimb.2021.657807

    PMID: 34568080

    Sample: serum
  12. Aging and Disease (2020) IF: 5.402
    Divergent Effect of Tacalcitol (PRI-2191) on Th17 Cells in 4T1 Tumor Bearing Young and Old Ovariectomized Mice

    DOI: 10.14336/AD.2019.0618

    Sample: CD4+ lymphocyte
  13. Stem Cells International (2022) IF: 5.443
    Mesenchymal Stem Cell-Secreted TGF-β1 Restores Treg/Th17 Skewing Induced by Lipopolysaccharide and Hypoxia Challenge via miR-155 Suppression

    DOI: 10.1155/2022/5522828

    Sample: CD4+ T cell
  14. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    JMJD3/H3K27me3 epigenetic modification regulates Th17/Treg cell differentiation in ulcerative colitis

    DOI: 10.1016/j.intimp.2022.109000

    PMID: 35777266

    Sample: colon tissue
  15. Nutrients (2021) IF: 5.719
    Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice

    DOI: 10.3390/nu13093209

    PMID: 34579088

    Sample: Splenocyte
  16. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Deacetylasperulosidic Acid Ameliorates Pruritus, Immune Imbalance, and Skin Barrier Dysfunction in 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis NC/Nga Mice

    DOI: 10.3390/ijms23010226

    PMID: 35008651

    Sample: Splenocyte
  17. Foods (2020) IF: 4.092
    Protective Effect of Prunus mume Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis

    DOI: 10.3390/foods10010058

    PMID: 33383792

    Sample: colon tissue,serum
  18. PROSTATE (2021) IF: 4.104
    Melatonin attenuates prostatic inflammation and pelvic pain via Sirt1-dependent inhibition of the NLRP3 inflammasome in an EAP mouse model

    DOI: 10.1002/pros.24214

    PMID: 34418127

    Sample: serum
  19. PROSTATE (2021) IF: 4.104
    4-Methylumbelliferone treatment and hyaluronan inhibition as a therapeutic strategy for chronic prostatitis

    DOI: 10.1002/pros.24205

    PMID: 34320251

    Sample: serum
  20. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2022) IF: 4.125
    Preventive and synbiotic effects of the soluble dietary fiber obtained from Lentinula edodes byproducts and Lactobacillus plantarum LP90 against dextran sulfate sodium-induced colitis in mice

    DOI: 10.1002/jsfa.12173

    PMID: 36054505

    Sample: serum,colon tissue
  21. Frontiers in Microbiology (2019) IF: 4.259
    Effect of Perillaldehyde on Prophylaxis and Treatment of Vaginal Candidiasis in a Murine Model

    DOI: 10.3389/fmicb.2019.01466

    PMID: 31333606

    Sample: Tissue homogenate
  22. Nutrients (2020) IF: 4.546
    Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function

    DOI: 10.3390/nu12010249

    PMID: 31963703

    Sample: Serum,Cell culture supernatant
  23. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Bixin protects mice against bronchial asthma though modulating PI3K/Akt pathway

    DOI: 10.1016/j.intimp.2021.108266

    PMID: 34678694

    Sample: BALF
  24. INTERNATIONAL IMMUNOPHARMACOLOGY (2019) IF: 4.932
    Arsenic trioxide improves Treg and Th17 balance by modulating STAT3 in treatment-naïve rheumatoid arthritis patients

    DOI: 10.1016/j.intimp.2019.05.001

    Sample: serum
  25. CELL PROLIFERATION (2018) IF: 4.936
    1,25(OH)2D3 inhibited Th17 cells differentiation via regulating the NF‐κB activity and expression of IL‐17

    DOI: 10.1111/cpr.12461

    Sample: Serum
  26. FASEB JOURNAL (2020) IF: 4.966
    CaMK4‐dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis

    DOI: 10.1096/fj.201902910RRR

    Sample: Cell culture supernatant,Tissue homogenate,serum
  27. Scientific Reports (2022) IF: 4.996
    Human umbilical cord mesenchymal stem cells derived extracellular vesicles regulate acquired immune response of lupus mouse in vitro

    DOI: 10.1038/s41598-022-17331-8

    PMID: 35908050

    Sample: hUCMSC
  28. EXPERIMENTAL BIOLOGY AND MEDICINE (2020) IF: 3.139
    Exosomes derived from synovial fibroblasts under hypoxia aggravate rheumatoid arthritis by regulating Treg/Th17 balance:

    DOI: 10.1177/1535370220934736

    Sample: Cell culture medium
  29. DIGESTIVE DISEASES AND SCIENCES (2019) IF: 3.199
    Role of Interleukin-17 in Pathogenesis of Intestinal Fibrosis in Mice

    DOI: 10.1007/s10620-019-05969-w

    Sample: blood,colonic tissue
  30. MOLECULES (2020) IF: 3.267
    A Herbal Mixture from Propolis, Pomegranate, and Grape Pomace Endowed with Anti-Inflammatory Activity in an In Vivo Rheumatoid Arthritis Model

    DOI: 10.3390/molecules25092255

    Sample: serum
  31. ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY (2013) IF: 3.449
    Therapeutic effects of anti–B7-H3 antibody in an ovalbumin-induced mouse asthma model

    DOI: 10.1016/j.anai.2013.06.030

    Sample: Bronchoalveolar Lavage Fluid
  32. Heliyon (2022) IF: 3.776
    A sesquiterpene isolated from the stems and leaves of Dioscorea opposita thunb. Transforms the composition of immune cells through ERβ in a mouse model of LPS-induced lung injury

    DOI: 10.1016/j.heliyon.2022.e10500

    Sample: Serum,lung tissue
  33. CYTOKINE (2022) IF: 3.861
    IL-17 upregulates MCP-1 expression via Act1 / TRAF6 / TAK1 in experimental autoimmune myocarditis

    DOI: 10.1016/j.cyto.2022.155823

    PMID: 35183823

    Sample: serum
  34. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2015) IF: 2.297
    Ghrelin ameliorates intestinal barrier dysfunction in experimental colitis by inhibiting the activation of nuclear factor-kappa B

    DOI: 10.1016/j.bbrc.2015.01.083

    Sample: Tissue homogenate
  35. CIRCULATION JOURNAL (2020) IF: 2.54
    Sustaining Circulating Regulatory T Cell Subset Contributes to the Therapeutic Effect of Paroxetine on Mice With Diabetic Cardiomyopathy

    DOI: 10.1253/circj.CJ-19-1182

    PMID: 32741881

    Sample: Serum
  36. JOURNAL OF MOLECULAR HISTOLOGY (2021) IF: 2.611
    MiRNA-182-5p aggravates experimental ulcerative colitis via sponging Claudin-2

    DOI: 10.1007/s10735-021-10021-1

    PMID: 34623552

    Sample: colonic tissue
  37. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
    Camelina sativa Oil Treatment Alleviates Castor Oil-Induced Diarrhea in ICR Mice by Regulating Intestinal Flora Composition

    DOI: 10.1155/2022/5394514

    PMID: 35178105

    Sample: plasma
  38. PLoS One (2018) IF: 2.766
    AT1-receptor blockade: Protective effects of irbesartan in cardiomyocytes under hypoxic stress

    DOI: 10.1371/journal.pone.0202297

    PMID: 30356256

    Sample: Cell culture supernatant
  39. ANDROLOGIA (2022) IF: 2.775
    IL-17 exacerbates experimental autoimmune prostatitis via CXCL1/CXCL2-mediated neutrophil infiltration

    DOI: 10.1111/and.14455

    PMID: 35560069

    Sample: prostate tissue
  40. INFLAMMATION (2018) IF: 2.884
    RNAi Silencing of HIF-1α Ameliorates Lupus Development in MRL/lpr Mice

    DOI: 10.1007/s10753-018-0815-6

    PMID: 30043119

    Sample: Serum
  41. MICROBIAL PATHOGENESIS (2020) IF: 2.914
    Exposure time determines the protective effect of Trichinella spiralis on experimental colitis

    DOI: 10.1016/j.micpath.2020.104263

    PMID: 32442663

    Sample: Serum
  42. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2019) IF: 2.965
    Gastrodin alleviates Tourette syndrome via Nrf‐2/HO‐1/HMGB1/NF‐кB pathway

    DOI: 10.1002/jbt.22389

    Sample: Tissue homogenate
  43. Current Medical Science (2020) IF: 1.273
    Role of Interleukin 17A in Aortic Valve Inflammation in Apolipoprotein E-deficient Mice

    DOI: 10.1007/s11596-020-2230-0

    PMID: 32862384

    Sample: Plasma
  44. Journal of Cosmetic Dermatology (2018) IF: 1.529
    Correlation between serum IL‐17A level and SALT score in patients with alopecia areata before and after NB‐UVB therapy

    DOI: 10.1111/jocd.12664

    Sample: Serum
  45. Journal of Huazhong University of Science and Technology-Medical Sciences (2016) IF: 0.838
    Peroxisome proliferator-activated receptor-γ agonist pioglitazone fails to attenuate renal fibrosis caused by unilateral ureteral obstruction in mice

    DOI: 10.1007/s11596-016-1539-1

    Sample: Tissue homogenate
Show AllShorten

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for Free Shipping 24T ELISA Kit
*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?

*Captcha: