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Mouse IL-17A(Interleukin 17A) ELISA Kit

Citations(18)Uniprot : Q62386

  • Cat.No.:E-EL-M0047

  • Reactivity: Mouse
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IL-17A in samples. No significant cross-reactivity or interference between Mouse IL-17A and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IL-17A concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-17A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-17A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-17A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-17A. You can calculate the concentration of Mouse IL-17A in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-17A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-17A were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 47.50 108.60 431.30 46.10 109.50 437.90
Standard deviation 2.80 5.40 22.90 2.30 5.00 16.20
CV (%) 5.89 4.97 5.31 4.99 4.57 3.70

Recovery

The recovery of Mouse IL-17A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-107 102
EDTA plasma (n=8) 91-106 97
Cell culture media (n=8) 94-105 100

Linearity

Samples were spiked with high concentrations of Mouse IL-17A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q62386
SynonymsCTLA8, CTLA-8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL17, IL-17, IL17A, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Oncotarget (2017) IF: 5.168
    Regulatory role of NKG2D+ NK cells in intestinal lamina propria by secreting double-edged Th1 cytokines in ulcerative colitis.

    DOI: 10.18632/oncotarget.22132

    PMID: 29228739

    		 Sample: Tissue homogenate
  2. Nutrients (2020) IF: 4.546
    Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function

    DOI: 10.3390/nu12010249

    PMID: 31963703

    		 Sample: Serum,Cell culture supernatant
  3. IF: 4.259
    Effect of Perillaldehyde on Prophylaxis and Treatment of Vaginal Candidiasis in a Murine Model
    Sample: Tissue homogenates
  4. Frontiers in Microbiology (2019) IF: 4.259
    Effect of Perillaldehyde on Prophylaxis and Treatment of Vaginal Candidiasis in a Murine Model

    DOI: 10.3389/fmicb.2019.01466

    PMID: 31333606

    		 Sample: Tissue homogenate
  5. Cell proliferation (2018) IF: 4.936
    1, 25 (OH) 2D3 inhibited Th17 cells differentiation via regulating the NF‐κB activity and expression of IL‐17.

    DOI: 10.1111/cpr.12461

    PMID: 29687949

    		 Sample: Serum
  6. Experimental Biology and Medicine (2020) IF: 3.139
    Exosomes derived from synovial fibroblasts under hypoxia aggravate rheumatoid arthritis by regulating Treg/Th17 balance

    DOI: 10.1177/1535370220934736

    PMID: 32615822

    		 Sample: Cell culture medium
  7. Journal of biochemical and molecular toxicology (2019) IF: 3.606
    Gastrodin alleviates Tourette syndrome via Nrf-2/HO-1/HMGB1/NF-кB pathway

    DOI: 10.1002/jbt.22389

    PMID: 31468582

    		 Sample: Tissue homogenate
  8. Annals of Allergy Asthma & Immunology (2013) IF: 3.728
    Therapeutic Effects Of Anti-B7-H3 Antibody In An Ovalbumin-Induced Mouse Asthma Model.

    DOI: 10.1016/j.anai.2013.06.030

    PMID: 24054363

    		 Sample: Bronchoalveolar Lavage Fluid
  9. Circulation Journal (2020) IF: 2.54
    Sustaining Circulating Regulatory T Cell Subset Contributes to the Therapeutic Effect of Paroxetine on Mice With Diabetic Cardiomyopathy

    DOI: 10.1253/circj.CJ-19-1182

    PMID: 32741881

    		 Sample: Serum
  10. Microbial Pathogenesis (2020) IF: 2.914
    Exposure time determines the protective effect of Trichinella spiralis on experimental colitis

    DOI: 10.1016/j.micpath.2020.104263

    PMID: 32442663

    		 Sample: Serum
  11. Inflammation (2018) IF: 2.884
    RNAi Silencing of HIF-1α Ameliorates Lupus Development in MRL/lpr Mice

    DOI: 10.1007/s10753-018-0815-6

    PMID: 30043119

    		 Sample: Serum
  12. PloS one (2018) IF: 2.766
    AT1-receptor blockade: Protective effects of irbesartan in cardiomyocytes under hypoxic stress

    DOI: 10.1371/journal.pone.0202297

    PMID: 30356256

    		 Sample: Cell culture supernatant
  13. Biochemical & Biophysical Research Communications (2015) IF: 2.466
    Ghrelin Ameliorates Intestinal Barrier Dysfunction In Experimental Colitis By Inhibiting The Activation Of Nuclear Factor-Kappa B.

    DOI: 10.1016/j.bbrc.2015.01.083

    PMID: 25634696

    		 Sample: Tissue homogenate
  14. Current Medical Science (2020) IF: 1.273
    Role of Interleukin 17A in Aortic Valve Inflammation in Apolipoprotein E-deficient Mice

    DOI: 10.1007/s11596-020-2230-0

    PMID: 32862384

    		 Sample: Plasma
  15. Journal of cosmetic dermatology (2018) IF: 1.529
    Correlation between serum IL‐17A level and SALT score in patients with alopecia areata before and after NB‐UVB therapy.

    DOI: 10.1111/jocd.12664

    PMID: 29707896

    		 Sample: Serum
  16. The FASEB Journal (2020)
    CaMK4-dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis

    DOI: 10.1096/fj.201902910RRR

    PMID: 32862457

    		 Sample: Cell culture supernatant,Tissue homogenate
  17. Iraqi Journal of Pharmaceutical Sciences (2018)
    Ameliorating Effect of Oral Guggulsterone Administration in Imiquimod-Induced Psoriasis in Mice
    Sample: Serum,Tissue homogenate
  18. Journal of Huazhong University Of Science And Technology-Medical Sciences (2016) IF: 0.964
    Peroxisome Proliferator-Activated Receptor-Γ Agonist Pioglitazone Fails To Attenuate Renal Fibrosis Caused By Unilateral Ureteral Obstruction In Mice.

    DOI: 10.1007/s11596-016-1539-1

    PMID: 26838738

    		 Sample: Tissue homogenate
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