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Mouse IL-6(Interleukin 6) ELISA Kit

Citations(136)Uniprot : P08505

Cat.No.:E-EL-M0044
Reactivity: Mouse

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse IL-6 in samples. No significant cross-reactivity or interference between Mouse IL-6 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse IL-6 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-6. You can calculate the concentration of Mouse IL-6 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-6 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-6 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	99.40	174.50	952.80	105.30	162.70	1040.70
Standard deviation	6.40	7.70	50.50	6.50	8.10	38.50
CV (%)	6.44	4.41	5.30	6.17	4.98	3.70

Recovery

The recovery of Mouse IL-6 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-100	93
EDTA plasma (n=8)	88-101	94
Cell culture media (n=8)	89-104	95

Linearity

Samples were spiked with high concentrations of Mouse IL-6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P08505
Synonyms	IL6, BSF2, HGF, HSF, IFNB2, CDF
Research Area	Cancer, Metabolism, Immunology, Neuroscience

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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DOI: 10.1016/j.bioorg.2018.10.073
PMID: 30448726	Sample: Cell culture medium

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DOI: 10.1016/j.fsi.2019.01.030
PMID: 30685466	Sample: Cell culture supernatant

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SIRT1 activation by butein attenuates sepsis-induced brain injury in mice subjected to cecal ligation and puncture via alleviating inflammatory and oxidative stress

DOI: 10.1016/j.taap.2018.10.013
PMID: 30336174	Sample: Serum,Tissue homogenate

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PMID: 30360404	Sample: Cell culture supernatant

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DOI: 10.3390/ijms20051103
PMID: 30836660	Sample: Serum

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4-Methylcoumarin-[5,6–g]-hesperetin attenuates inflammatory responses in alcoholic hepatitis through PPAR-γ activation

DOI: 10.1016/j.tox.2019.04.004
PMID: 30951781	Sample: Serum,Cell culture supernatant

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Pretreatment of exosomes derived from hUCMSCs with TNF-α ameliorates acute liver failure by inhibiting the activation of NLRP3 in macrophage

DOI: 10.1016/j.lfs.2020.117401
PMID: 32035931	Sample: Whole Blood,Tissue homogenate

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Ameliorative Effects of Suhuang Antitussive Capsule on LPS-Induced Sputum Obstruction in Mice through Promoting HGF Secretion via an Intestinal Endocrine-Dependent Pathway

DOI: 10.3389/fphar.2019.01422
	Sample: Serum,Tissue homogenate

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DOI: 10.3390/molecules24203663
PMID: 31614601	Sample: Cell culture supernatant

Biomedicine & Pharmacotherapy (2018) IF: 3.457

The protective effect of formononetin on cognitive impairment in streptozotocin (STZ)-induced diabetic mice.

DOI: 10.1016/j.biopha.2018.07.063
PMID: 30119194	Sample: Serum,Tissue homogenate

Neuroscience (2017) IF: 3.277

Blocking The CD38/cADPR Pathway Plays A Double-edged Role In LPS Stimulated Microglia.

DOI: 10.1016/j.neuroscience.2017.08.010
PMID: 28807785	Sample: Cell culture supernatant

Acta Pharmacologica Sinica (2017) IF: 3.223

Osthole Prevents Acetaminophen-Induced Liver Injury In Mice.

DOI: 10.1038/aps.2017.129
PMID: 29022574	Sample: Tissue homogenate

Innate Immunity (2021) IF: 2.68

Chrysosplenol D protects mice against LPS-induced acute lung injury by inhibiting oxidative stress, inflammation, and apoptosis via TLR4-MAPKs/NF-κB signaling pathways

DOI: 10.1177/17534259211051069
PMID: 34806444	Sample: Bronchoalveolar Lavage Fluid

Fitoterapia (2021) IF: 2.88

Structure elucidation and anti-inflammatory mechanism of difengpienol C, a new neolignan isolated from Illicium difengpi

DOI: 10.1016/j.fitote.2021.104949
PMID: 34087408	Sample: Cell culture supernatant

Journal of Neuroscience Methods (2020) IF: 2.214

Frequent exposure to varied home cage sizes alters pain sensitivity and some key inflammation-related biomarkers

DOI: 10.1016/j.jneumeth.2020.108890
PMID: 32768413	Sample: Tissue homogenate

Bioscience Reports (2020) IF: 2.942

Atractylon treatment prevents sleep-disordered breathing-induced cognitive dysfunction by suppression of chronic intermittent hypoxia-induced M1 microglial activation

DOI: 10.1042/BSR20192800
PMID: 32490526	Sample: Cell culture supernatant

Molecular Medicine Reports (2020) IF: 2.1

Osthole inhibits proliferation of kainic acid-activated BV-2 cells by modulating the Notch signaling pathway

DOI: 10.3892/mmr.2020.11455
PMID: 33000274	Sample: Cell culture supernatant

Molecular Medicine Reports (2020) IF: 2.1

Astaxanthin suppresses lipopolysaccharide-induced myocardial injury by regulating MAPK and PI3K/AKT/mTOR/GSK3β signaling

DOI: 10.3892/mmr.2020.11443
PMID: 32945516	Sample: Serum

Brain and behavior (2020) IF: 2.091

MicroRNA-345-5p regulates depression by targeting suppressor of cytokine signaling 1

DOI: 10.1002/brb3.1653
PMID: 32730696	Sample: Cell culture supernatant

Microbial Pathogenesis (2020) IF: 2.914

Curcumin ameliorates Staphylococcus aureus-induced mastitis injury through attenuating TLR2-mediated NF-κB activation

DOI: 10.1016/j.micpath.2020.104054
PMID: 32061917	Sample: Serum

Molecular Medicine Reports (2020) IF: 2.1

Coenzyme Q10 alleviates sevoflurane-induced neuroinflammation by regulating the levels of apolipoprotein E and phosphorylated tau protein in mouse hippocampal neurons

DOI: 10.3892/mmr.2020.11131
PMID: 32377738	Sample: Tissue homogenate

Poultry science (2019) IF: 2.659

Anti-inflammatory effect of preserved egg with simulated gastrointestinal digestion on LPS-stimulated RAW264.7 cells

DOI: 10.3382/ps/pez243
PMID: 31041437	Sample: Cell culture supernatant

Molecular medicine reports (2018) IF: 2.1

Dioscin prevents LPS-induced acute lung injury through inhibiting the TLR4/MyD88 signaling pathway via upregulation of HSP70

DOI: 10.3892/mmr.2018.8667
PMID: 29512786	Sample: Serum,Tissue homogenate

Medicine (2018) IF: 2.028

Effect of gap junctions on RAW264. 7 macrophages infected with H37Rv

DOI: 10.1097/MD.0000000000012125
PMID: 30170447	Sample: Cell culture supernatant

Inflammation (2018) IF: 2.884

miR-590-3p Attenuates Acute Kidney Injury by Inhibiting Tumor Necrosis Factor Receptor-Associated Factor 6 in Septic Mice

DOI: 10.1007/s10753-018-0921-5
PMID: 30392071	Sample: Serum

Bioscience reports (2018) IF: 2.899

Maggot protein ameliorates dextran sulphate sodium-induced ulcerative colitis in mice

DOI: 10.1042/BSR20181799
PMID: 30393231	Sample: Serum

Biochemical and biophysical research communications (2019) IF: 2.559

Eosinophils attenuate arthritis by inducing M2 macrophage polarization via inhibiting the IkB/P38 MAPK signaling pathway

DOI: 10.1016/j.bbrc.2018.12.010
PMID: 30528734	Sample: Tissue homogenate,Cell culture supernatant

Inflammation (2018) IF: 2.884

Resveratrol Protects Against Renal Damage via Attenuation of Inflammation and Oxidative Stress in High-Fat-Diet-Induced Obese Mice

DOI: 10.1007/s10753-018-0948-7
PMID: 30554371	Sample: Tissue homogenate

Bioscience reports (2018) IF: 2.899

Association of IL-6, IL-8, MMP-13 gene polymorphisms with knee osteoarthritis susceptibility in the Chinese Han population

DOI: 10.1042/BSR20181346
PMID: 30635366	Sample: Tissue homogenate

Steroids (2019) IF: 2.523

Synthesis and Evaluation of Novel D-ring Substituted Steroidal Pyrazolines as Potential Anti-inflammatory Agents

DOI: 10.1016/j.steroids.2019.03.012
PMID: 30951758	Sample: Cell culture supernatant

Journal of the Science of Food and Agriculture (2019) IF: 2.379

Effect and mechanism of citral against methicillin-resistant Staphylococcus aureus in vivo

DOI: 10.1002/jsfa.9677
PMID: 30891759	Sample: Serum

Journal of cellular biochemistry (2019) IF: 2.959

Attractylone Attenuates Sepsis-Associated Encephalopathy and Cognitive Dysfunction by Inhibiting Microglial Activation and Neuroinflammation

DOI: 10.1002/jcb.27983
PMID: 30672013	Sample: Cell culture supernatant,Tissue homogenate

Gene (2019) IF: 2.498

The inhibition of microRNA-203 on ischemic reperfusion injury after total knee arthroplasty via suppressing MYD88-mdiated toll-like receptor signaling pathway

DOI: 10.1016/j.gene.2019.02.030
PMID: 30772517	Sample: Tissue homogenate

Inflammation (2019) IF: 2.884

Sinomenine Attenuates Cartilage Degeneration by Regulating miR-223-3p/NLRP3 Inflammasome Signaling

DOI: 10.1007/s10753-019-00986-3
PMID: 30847744	Sample: Serum,Cell culture supernatant

Biomedical and Environmental Sciences (2018) IF: 2.518

Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice.

DOI: 10.3967/bes2018.101
PMID: 30423277	Sample: Plasma,Tissue homogenate

Plos One (2017) IF: 2.806

Therapeutic Effect Of imiquimod On Dextran Sulfate Sodium-induced Ulcerative Colitis In Mice.

DOI: 10.1371/journal.pone.0186138
PMID: 29049372	Sample: Serum

PloS one (2017) IF: 2.806

Chronic administration of mitochondrion-targeted peptide SS-31 prevents atherosclerotic development in ApoE knockout mice fed Western diet.

DOI: 10.1371/journal.pone.0185688
PMID: 28961281	Sample: Serum

International Immunopharmacology (2017) IF: 2.956

Wogonin Attenuates Inflammation By Activating PPAR-γ In Alcoholic Liver Disease.

DOI: 10.1016/j.intimp.2017.06.013
PMID: 28646664	Sample: Serum,Cell culture supernatant

Molecules (2017) IF: 2.861

Capparis Spinosa Fruit Ethanol Extracts Exert Different Effects On The Maturation Of Dendritic Cells.

DOI: 10.3390/molecules22010097
PMID: 28067853	Sample: Cell culture supernatant

Biochemical & Biophysical Research Communications (2015) IF: 2.466

Ghrelin Ameliorates Intestinal Barrier Dysfunction In Experimental Colitis By Inhibiting The Activation Of Nuclear Factor-Kappa B.

DOI: 10.1016/j.bbrc.2015.01.083
PMID: 25634696	Sample: Tissue homogenate

Food Science & Nutrition (2020) IF: 1.797

Subcutaneous administration of casein attenuates atherosclerotic progression in male apoE -/- mice fed with high-fat diet

DOI: 10.1002/fsn3.1638
PMID: 32724618	Sample: Plasma

Experimental and Therapeutic Medicine (2020) IF: 1.785

Clostridium butyricum protects intestinal barrier function via upregulation of tight junction proteins and activation of the Akt/mTOR signaling pathway in a mouse model of dextran sodium sulfate-induced colitis

DOI: 10.3892/etm.2020.9138
PMID: 32934675	Sample: Serum

Current Medical Science (2020) IF: 1.273

Role of Interleukin 17A in Aortic Valve Inflammation in Apolipoprotein E-deficient Mice

DOI: 10.1007/s11596-020-2230-0
PMID: 32862384	Sample: Plasma

Asian Pacific Journal of Tropical Medicine (2019) IF: 1.94

Alpinia officinarum Hance extract alleviates particulate matter-induced lung injury in mice

DOI: 10.4103/1995-7645.272487
	Sample: Serum,Bronchoalveolar Lavage Fluid,Tissue homogena

Medical Science Monitor: International Medical Journal of Experimental and Clinical Research (2020) IF: 1.918

The Effects of RKI-1447 in a Mouse Model of Nonalcoholic Fatty Liver Disease Induced by a High-Fat Diet and in HepG2 Human Hepatocellular Carcinoma Cells Treated with Oleic Acid

DOI: 10.12659/MSM.919220
PMID: 32026851	Sample: Serum,Cell culture supernatant,Tissue homogenate

Current medical science (2019) IF: 1.273

Berberine Attenuates Cigarette Smoke Extract-induced Airway Inflammation in Mice: Involvement of TGF-β1/Smads Signaling Pathway

DOI: 10.1007/s11596-019-2101-8
PMID: 31612392	Sample: Bronchoalveolar Lavage Fluid

Canadian journal of physiology and pharmacology (2019) IF: 1.946

Role of Brd4 in the production of inflammatory cytokines in mouse macrophages treated with titanium particles

DOI: 10.1139/cjpp-2019-0142
PMID: 31330113	Sample: Cell culture supernatant

Experimental and therapeutic medicine (2018) IF: 1.41

miR-148a inhibits pro-inflammatory cytokines released by intervertebral disc cells by regulating the p38/MAPK pathway

DOI: 10.3892/etm.2018.6516
PMID: 30186499	Sample: Cell culture supernatant

Medical molecular morphology (2019) IF: 1.217

The therapeutic effects of resveratrol on hepatic steatosis in high-fat diet-induced obese mice by improving oxidative stress, inflammation and lipid-related gene transcriptional expression

DOI: 10.1007/s00795-019-00216-7
PMID: 30673851	Sample: Serum,Tissue homogenate

Cellular & molecular biology letters (2019) IF: 1.291

Tetrahydroxy stilbene glucoside alleviates palmitic acid-induced inflammation and apoptosis in cardiomyocytes by regulating miR-129-3p/Smad3 signaling

DOI: 10.1186/s11658-018-0125-x
PMID: 30820195	Sample: Cell culture supernatant

Journal of biochemical and molecular toxicology (2019) IF: 1.837

Gastrodin attenuates neuroinflammation in DOI-induce Tourette syndrome in rats

DOI: 10.1002/jbt.22302
PMID: 30790395	Sample: Serum,Tissue homogenate

Cellular & molecular biology letters (2019) IF: 1.291

Honokiol alleviates sepsis-induced acute kidney injury in mice by targeting the miR-218-5p/heme oxygenase-1 signaling pathway

DOI: 10.1186/s11658-019-0142-4
PMID: 30833971	Sample: Serum

Reproduction, Fertility and Development (2019) IF: 1.723

Notch signalling regulates steroidogenesis in mouse ovarian granulosa cells

DOI: 10.1071/RD18281
PMID: 30827331	Sample: Cell culture medium

Oncology Letters (2018) IF: 1.39

The Association Between Inflammation, The Microbiome and Urethane-induced Pulmonary Adenocarcinoma.

DOI: 10.3892/ol.2018.8167
PMID: 29725395

Molecular Medicine Reports (2018) IF: 1.692

Oral supplementation with ursolic acid ameliorates sepsis-induced acute kidney injury in a mouse model by inhibiting oxidative stress and inflammatory responses.

DOI: 10.3892/mmr.2018.8767
PMID: 29568928

International Journal of Clinical and Experimental Pathology (2015) IF: 1.706

An Unusual Case Of Iron Deficiency Anemia Is Associated With Extremely Low Level Of Transferrin Receptor.


PMID: 26339443	Sample: Serum

International Journal of Clinical and Experimental Medicine (2015) IF: 1.069

Sulfated Modification Promotes The Immunomodulatory Bioactivities Of Lyciumbarbarum Polysaccharides In Vitro.


PMID: 26884954	Sample: Cell culture supernatant

Journal of Oleo Science (2015) IF: 1.076

A Comparative Study Of The Effects Upon LPS Induced Macrophage RAW264.7 Inflammation In Vitro Of The Lipids Of Hippocampus Trimaculatus Leach.

DOI: 10.5650/jos.ess15190
PMID: 26582156	Sample: Cell culture supernatant

Medical Science Monitor: International Medical Journal of Experimental and Clinical Research (2020)

Methamphetamine induced neuroinflammation in mouse brain and microglial cell line BV2: Roles of the TLR4/TRIF/Peli1 signaling axis

DOI: 10.1016/j.toxlet.2020.07.028
PMID: 32768653	Sample: Cell culture supernatant,Tissue homogenate

Advances in Pharmacological and Pharmaceutical Sciences (2020)

Antihypernociceptive, Anxiolytic, and Antidepressant Properties of Aqueous and Ethanol Extracts of Dissotis thollonii Cogn. (Melastomataceae) in Mice

DOI: 10.1155/2020/8886894
PMID: 33015629	Sample: Serum,Tissue homogenate

The FASEB Journal (2020)

CaMK4-dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis

DOI: 10.1096/fj.201902910RRR
PMID: 32862457	Sample: Cell culture supernatant,Tissue homogenate

Jurnal Kedokteran Brawijaya (2020)

The Effects of Thyme on IL-6 Level and the Bacterial Count in the Brain and Spleen of Mice Infected by Methicillin-Resistant Staphylococcus aureus (MRSA)

DOI: 10.21776/ub.jkb.2020.031.01.1
	Sample: Plasma

bioRxiv (2020)

Fecal microbiota transplantation reconstructs the gut microbiota of septic mice and protects the intestinal mucosal barrier

DOI: 10.1101/2020.06.22.164541
	Sample: Serum

SSRN (2020)

Salidroside Attenuates Liver Injury in Diabetes Mice

DOI: 10.2139/ssrn.3546025
	Sample: Serum,Cell culture supernatant,Tissue homogenate

Journal of Cellular and Molecular Medicine (2020)

Polysaccharides from Dicliptera chinensis ameliorate liver disturbance by regulating TLR-4/NF-κB and AMPK/Nrf2 signalling pathways

DOI: 10.1111/jcmm.15286
PMID: 32337831	Sample: Serum,Tissue homogenate

Open access Macedonian journal of medical sciences (2019)

Neuroprotective Activity of Evolvulus alsinoides & Centella asiatica Ethanolic Extracts in Scopolamine-Induced Amnesia in Swiss Albino Mice

DOI: 10.3889/oamjms.2019.247
PMID: 31049081	Sample: Tissue homogenate

Veterinary world (2019)

Golden sea cucumber extract revives glucose transporter-4 and interleukin-6 protein level in diabetic mouse muscle

DOI: 10.14202/vetworld.2019.684-688
PMID: 31327904	Sample: Tissue homogenate

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Q&A

R*********sSubmitted [ Jul 05 2021 ]

Asked: Hello, Is E-EL-M0044 kit suitable for tissue homogenates?

adminSubmitted [ Jul 05 2021 ]

Answered: The E-EL-M0044 kit is suitable for mouse tissue homogenates samples.

adminSubmitted [ Jul 05 2021 ]

Answered:

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