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Cat.No.:E-EL-M1382
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 3.13-200 pg/ml |
Sensitivity | 1.88 pg/ml |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Mouse INS in samples. No significant cross-reactivity or interference between Mouse INS and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Mouse INS concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse INS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse INS were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/ml) | 9.36 | 24.03 | 91.53 | 8.46 | 24.39 | 87.80 |
Standard deviation | 0.52 | 1.17 | 4.07 | 0.42 | 1.20 | 4.79 |
CV (%) | 5.56 | 4.87 | 4.45 | 4.96 | 4.92 | 5.46 |
The recovery of Mouse INS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 85-98 | 91 |
EDTA plasma (n=8) | 85-95 | 90 |
Samples were spiked with high concentrations of Mouse INS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P01325 P01326
|
Synonyms | IDDM2, ILPR, IRDN, MODY10 |
Research Area | Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
PMID: 36572670 |
Sample: serum |
Sample: Serum |
PMID: 35511344 |
Sample: plasma |
Sample: serum |
Sample: serum,cortical |
Sample: serum |
Sample: serum |
DOI: 10.1039/D2FO03687H |
|
Sample: serum |
PMID: 35202915 |
Sample: blood |
DOI: 10.1155/2021/4706410 |
|
PMID: 34745416 |
Sample: serum |
DOI: 10.1155/2020/6538930 |
|
PMID: 32774682 |
Sample: Serum |
Sample: serum |
PMID: 33571514 |
Sample: Serum |
PMID: 33915130 |
Sample: Plasma |
PMID: 35751919 |
Sample: serum |
Sample: plasma |
PMID: 30177151 |
Sample: Plasma |
Sample: plasma |
Sample: serum |
Sample: Serum |
PMID: 30521378 |
Sample: Serum |
DOI: 10.1039/D1FO02714J |
|
Sample: blood |
DOI: 10.1038/srep16262 |
|
Sample: Serum |
Sample: serum |
DOI: 10.3390/v14122781 |
|
Sample: Pancreases,plasma |
Sample: serum |
DOI: 10.3390/ijms23074015 |
|
PMID: 35409375 |
Sample: serum |
Sample: serum |
PMID: 31485878 |
Sample: Serum |
PMID: 34689672 |
Sample: plasma |
Sample: plasma |
Sample: serum |
PMID: 32309947 |
Sample: Serum,Tissue homogenate |
Sample: serum |
PMID: 30605818 |
Sample: Serum |
PMID: 34537282 |
Sample: plasma |
DOI: 10.1002/ddr.21966 |
|
PMID: 35749612 |
Sample: serum |
PMID: 35921919 |
Sample: serum |
Sample: Serum |
Sample: serum |
PMID: 35779798 |
Sample: Serum |
Sample: serum |
Sample: plasma |
DOI: 10.2147/DMSO.S372192 |
|
PMID: 36060789 |
Sample: serum |
PMID: 33307381 |
Sample: serum |
DOI: 10.1039/C8FO00444G |
|
Sample: Plasma |
PMID: 32652086 |
Sample: Serum |
PMID: 31254565 |
Sample: Serum |
PMID: 35041127 |
Sample: serum |
Sample: serum |
Sample: Plasma |
PMID: 31545275 |
Sample: Serum,Urine |
PMID: 35461164 |
Sample: plasma |
Sample: plasma |
Sample: plasma |
DOI: 10.14670/hh-18-358 |
|
Sample: plasma |
Sample: Serum |
PMID: 32725591 |
Sample: Serum |
DOI: 10.1155/2021/9968463 |
|
PMID: 34987599 |
Sample: serum |
DOI: 10.1111/jfbc.14280 |
|
PMID: 35746862 |
Sample: serum |
Sample: Plasma |
Sample: serum |
Sample: Serum |
PMID: 33292347 |
Sample: serum |
Sample: Plasma |
PMID: 30673851 |
Sample: Serum,Tissue homogenate |
DOI: 10.1002/fsn3.2040 |
|
Sample: serum |
Sample: plasma |