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Mouse INS(Insulin) ELISA Kit

  • Cat.No.:E-EL-M1382

  • Reactivity: Mouse

To Purchase E-EL-M1382

Size:
  • 96T
  • 24T
Price: $609
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 3.13-200 pg/ml
Sensitivity 1.88 pg/ml
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse INS in samples. No significant cross-reactivity or interference between Mouse INS and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse INS concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse INS. You can calculate the concentration of Mouse INS in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse INS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse INS were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/ml) 9.36 24.03 91.53 8.46 24.39 87.80
Standard deviation 0.52 1.17 4.07 0.42 1.20 4.79
CV (%) 5.56 4.87 4.45 4.96 4.92 5.46

Recovery

The recovery of Mouse INS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-98 91
EDTA plasma (n=8) 85-95 90
Cell culture media (n=8) 92-99 95

Linearity

Samples were spiked with high concentrations of Mouse INS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsIDDM2, ILPR, IRDN, MODY10
Research AreaCancer

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Bone research (2018) IF: 9.326000000
    Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines.

    DOI: 10.1038/s41413-018-0012-0

    PMID: 29619269

  2. Oxidative medicine and cellular longevity (2020) IF: 5.076
    Andrographolide Exerts Antihyperglycemic Effect through Strengthening Intestinal Barrier Function and Increasing Microbial Composition of Akkermansia muciniphila

    DOI: 10.1155/2020/6538930

    PMID: 32774682

    Sample: Serum
  3. Carbohydrate polymers (2018) IF: 5.158
    Polysaccharides extracted from Phellinus linteus ameliorate high-fat high-fructose diet induced insulin resistance in mice

    DOI: 10.1016/j.carbpol.2018.07.086

    PMID: 30177151

    Sample: Plasma
  4. Carbohydrate Polymers (2018) IF: 5.158
    Polysaccharides extracted from Phellinus linteus ameliorate high-fat high-fructose diet induced insulin resistance in mice.

    DOI: 10.1016/j.carbpol.2018.07.086

    PMID: 30177151

    Sample: Plasma
  5. Journal of Agricultural and Food Chemistry (2020) IF: 4.192
    Modulation of Gut Microbiota by Fucoxanthin During Alleviation of Obesity in High-Fat Diet-Fed Mice

    DOI: 10.1021/acs.jafc.0c01467

    PMID: 32309947

    Sample: Serum,Tissue homogenate
  6. Biomedicine & Pharmacotherapy (2019) IF: 4.545
    Therapeutic potential of NaoXinTong Capsule on the developed diabetic nephropathy in db/db mice

    DOI: 10.1016/j.biopha.2019.109389

    PMID: 31545275

    Sample: Serum,Urine
  7. Journal of Agricultural and Food Chemistry (2019) IF: 4.192
    Pelargonidin-3- O-glucoside Derived from Wild Raspberry Exerts Antihyperglycemic Effect by Inducing Autophagy and Modulating Gut Microbiota

    DOI: 10.1021/acs.jafc.9b03338

    PMID: 31322351

    Sample: Serum
  8. Pharmacological research (2018) IF: 4.897
    Baicalin regulates SirT1/STAT3 pathway and restrains excessive hepatic glucose production

    DOI: 10.1016/j.phrs.2018.08.018

    PMID: 30144531

    Sample: Cell culture supernatant
  9. Environmental Pollution (2019) IF: 4.358
    Effects of triphenyl phosphate exposure during fetal development on obesity and metabolic dysfunctions in adult mice: Impaired lipid metabolism and intestinal dysbiosis

    DOI: 10.1016/j.envpol.2018.12.053

    PMID: 30605818

    Sample: Serum
  10. Apoptosis (2019) IF: 4.021
    Rotenone Protects Against β-cell Apoptosis and Attenuates Type 1 Diabetes Mellitus

    DOI: 10.1007/s10495-019-01566-4

    PMID: 31485878

    Sample: Serum
  11. Pharmacological Research (2018) IF: 4.897
    Baicalin regulates SirT1/STAT3 pathway and restrains excessive hepatic glucose production.

    DOI: 10.1016/j.phrs.2018.08.018

    PMID: 30144531

  12. Frontiers in Pharmacology (2017) IF: 4.4
    Psoralea Corylifolia L. Attenuates Nonalcoholic Steatohepatitis In Juvenile Mouse.

    DOI: 10.3389/fphar.2017.00876

    PMID: 29249967

    Sample: Serum
  13. Scientific Reports (2015) IF: 4.259
    Mitochondria-Related miR-141-3p Contributes To Mitochondrial Dysfunction In HFD-Induced Obesity By Inhibiting PTEN.

    DOI: 10.1038/srep16262

    PMID: 26548909

    Sample: Serum
  14. Toxicology and Applied Pharmacology (2020) IF: 3.347
    Role of TRPV1/TRPV3 channels in olanzapine-induced metabolic alteration: Possible involvement in hypothalamic energy-sensing, appetite regulation, inflammation and mesolimbic pathway

    DOI: 10.1016/j.taap.2020.115124

    PMID: 32652086

    Sample: Serum
  15. Toxicology and applied pharmacology (2019) IF: 3.347
    Modeling of antipsychotic-induced metabolic alterations in mice: An experimental approach precluding psychosis as a predisposing factor

    DOI: 10.1016/j.taap.2019.114643

    PMID: 31254565

    Sample: Serum
  16. Food & function (2018) IF: 3.289
    Diet induced maternal obesity affects offspring gut microbiota, which persists into young adulthood.

    DOI: 10.1039/c8fo00444g

    PMID: 30040095

    Sample: Plasma
  17. Journal of Agricultural and Food Chemistry (2015) IF: 3.154
    Purified Betacyanins From Hylocereus Undatus Peel Ameliorate Obesity And Insulin Resistance In High-Fat-Diet-Fed Mice.

    DOI: 10.1021/acs.jafc.5b05177

    PMID: 26653843

    Sample: Serum
  18. Biochemical and Biophysical Research Communications (2020) IF: 2.985
    Lpl-C310R mutation is associated with impaired glucose tolerance and endoplasmic reticulum stress in skeletal muscle

    DOI: 10.1016/j.bbrc.2020.06.055

    PMID: 99999999

    Sample: Plasma
  19. Nutrition Research (2020) IF: 2.767
    A?aí seed extract prevents the renin-angiotensin system activation, oxidative stress and inflammation in white adipose tissue of high-fat diet-fed mice

    DOI: 10.1016/j.nutres.2020.05.006

    PMID: 32610256

    Sample: Plasma
  20. Nutrition Research (2016) IF: 2.737
    Mulberry Ethanol Extract Attenuates Hepatic Steatosis And Insulin Resistance In High-Fat Diet-Fed Mice.

    DOI: 10.1016/j.nutres.2016.01.011

    PMID: 27262537

    Sample: Serum
  21. Medical molecular morphology (2019) IF: 1.217
    The therapeutic effects of resveratrol on hepatic steatosis in high-fat diet-induced obese mice by improving oxidative stress, inflammation and lipid-related gene transcriptional expression

    DOI: 10.1007/s00795-019-00216-7

    PMID: 30673851

    Sample: Serum,Tissue homogenate
  22. Porto Biomedical Journal (2020)
    An albino mouse model of nonalcoholic fatty liver disease induced using high-fat liquid "Lieber-DeCarli" diet: a preliminary investigation

    DOI: 10.1097/j.pbj.0000000000000071

    PMID: 32734012

    Sample: Plasma,Tissue homogenate
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