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Cat.No.:E-EL-M2614
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Assay type | Sandwich-ELISA |
Format | 96T |
Assay time | 3.5h |
Detection range | 0.31-20 ng/mL |
Sensitivity | 0.19 ng/mL |
Sample type &Sample volume | cell culture supernatant and tissue homogenate; 100μL |
Specificity | This kit recognizes Mouse INS in samples. No significant cross-reactivity or interference between Mouse INS and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Mouse INS concentrations in cell culture supernatant and tissue homogenate. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse INS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse INS were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 1.00 | 2.30 | 8.50 | 1.00 | 2.30 | 8.70 |
Standard deviation | 0.10 | 0.10 | 0.40 | 0.10 | 0.10 | 0.30 |
CV (%) | 10.00 | 4.35 | 4.71 | 10.00 | 4.35 | 3.45 |
The recovery of Mouse INS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Cell culture media (n=8) | 95-110 | 101 |
Samples were spiked with high concentrations of Mouse INS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
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1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
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PMID: 34556631 |
Sample: Krt5+ cell |
Sample: serum,cortical |
PMID: 32309947 |
Sample: Serum,Tissue homogenate |
PMID: 30144531 |
Sample: Cell culture supernatant |
Sample: MIN6 cells |
DOI: 10.1155/2021/5514175 |
|
PMID: 34211563 |
Sample: hippocampu |
Sample: Cell culture supernatant |
Sample: MIN6 cells |
PMID: 35302175 |
Sample: MIN6 cell |
PMID: 30673851 |
Sample: Serum,Tissue homogenate |
PMID: 36404863 |
Sample: liver tissue |