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Mouse IP-10/CXCL10(Interferon Gamma Induced Protein 10kDa) ELISA Kit

Citations(13)Uniprot : P17515

Cat.No.:E-EL-M0021
Reactivity: Mouse

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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse IP-10/CXCL10 in samples. No significant cross-reactivity or interference between Mouse IP-10/CXCL10 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse IP-10/CXCL10 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IP-10/CXCL10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IP-10/CXCL10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IP-10/CXCL10. You can calculate the concentration of Mouse IP-10/CXCL10 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IP-10/CXCL10 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IP-10/CXCL10 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	97.30	250.00	728.80	87.90	251.00	683.90
Standard deviation	5.30	14.30	36.40	5.50	12.00	31.50
CV (%)	5.45	5.72	4.99	6.26	4.78	4.61

Recovery

The recovery of Mouse IP-10/CXCL10 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	84-96	91
EDTA plasma (n=8)	86-98	93
Cell culture media (n=8)	89-103	96

Linearity

Samples were spiked with high concentrations of Mouse IP-10/CXCL10 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P17515
Synonyms	C7, IFI10, INP10, SCYB10, crg-2, gIP-10, mob-1
Research Area	Cancer, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Journal for ImmunoTherapy of Cancer (2022) IF: 12.469
Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis

DOI: 10.1136/jitc-2021-004381

PMID: 35738798
Sample: B16F10 cell-xenograft
Bioengineering & Translational Medicine (2022) IF: 10.711
Baicalein self-microemulsion based on drug–phospholipid complex for the alleviation of cytokine storm

DOI: 10.1002/btm2.10357

Sample: plasma
CARBOHYDRATE POLYMERS (2022) IF: 10.723
Aloe polymeric acemannan inhibits the cytokine storm in mouse pneumonia models by modulating macrophage metabolism

DOI: 10.1016/j.carbpol.2022.120032

PMID: 36184177
Sample: bronchoalveolar fluid,RAW264.7 cell
JOURNAL OF INVESTIGATIVE DERMATOLOGY (2021) IF: 8.551
Successful Treatment of Vitiligo with Cold Atmospheric Plasma‒Activated Hydrogel

DOI: 10.1016/j.jid.2021.04.019

PMID: 34029575
Sample: Serum,Tissue homogenate
Frontiers in Immunology (2021) IF: 7.561
Pathogenic Roles of CXCL10 in Experimental Autoimmune Prostatitis by Modulating Macrophage Chemotaxis and Cytokine Secretion

DOI: 10.3389/fimmu.2021.706027

PMID: 34659199
Sample: plasma,prostate tissue
BIOLOGICAL PROCEDURES ONLINE (2022) IF: 7.717
Angiotensin-converting enzyme 2 identifies immuno-hot tumors suggesting angiotensin-(1–7) as a sensitizer for chemotherapy and immunotherapy in breast cancer

DOI: 10.1186/s12575-022-00177-9

Sample: 4T1 cell-xenograft
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2022) IF: 5.31
Activation of the STING-IRF3 pathway involved in psoriasis with diabetes mellitus

DOI: 10.1111/jcmm.17236

Sample: serum
FEBS LETTERS (2022) IF: 4.124
The Microglial membrane receptor TREM2 mediates exosome secretion to promote phagocytosis of amyloid-β by microglia

DOI: 10.1002/1873-3468.14336

PMID: 35292963
Sample: Bv2 cell
INFLAMMATION RESEARCH (2021) IF: 4.575
Inhibition of fibroblast growth factor-inducible 14 attenuates experimental tubulointerstitial fibrosis and profibrotic factor expression of proximal tubular epithelial cells

DOI: 10.1007/s00011-021-01455-0

PMID: 33755760
Sample: Tissue homogenate
INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
The selective STING inhibitor H-151 preserves myocardial function and ameliorates cardiac fibrosis in murine myocardial infarction

DOI: 10.1016/j.intimp.2022.108658

PMID: 35278833
Sample: serum
INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 4.932
COX-2-PGE2 signaling pathway contributes to hippocampal neuronal injury and cognitive impairment in PTZ-kindled epilepsy mice

DOI: 10.1016/j.intimp.2020.106801

Sample: Tissue homogenate
ANTICANCER RESEARCH (2022) IF: 2.435
Inhibition of STAT3 Promotes Effector T Cell Infiltration But also Immunosuppression in the HCC Tumor Microenvironment

DOI: 10.21873/anticanres.16027

Sample: RIL-175 cell
RESEARCH IN VETERINARY SCIENCE (2019) IF: 1.751
Amelioration effects of Kaempferol on immune response following chronic intermittent cold-stress

DOI: 10.1016/j.rvsc.2019.08.012

PMID: 31412308
Sample: Serum,Tissue homogenate

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