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Mouse IP-10/CXCL10(Interferon Gamma Induced Protein 10kDa) ELISA Kit

  • Cat.No.:E-EL-M0021

  • Reactivity: Mouse

To Purchase E-EL-M0021

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IP-10/CXCL10 in samples. No significant cross-reactivity or interference between Mouse IP-10/CXCL10 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IP-10/CXCL10 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IP-10/CXCL10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IP-10/CXCL10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IP-10/CXCL10. You can calculate the concentration of Mouse IP-10/CXCL10 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IP-10/CXCL10 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IP-10/CXCL10 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 97.30 250.00 728.80 87.90 251.00 683.90
Standard deviation 5.30 14.30 36.40 5.50 12.00 31.50
CV (%) 5.45 5.72 4.99 6.26 4.78 4.61

Recovery

The recovery of Mouse IP-10/CXCL10 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-96 91
EDTA plasma (n=8) 86-98 93
Cell culture media (n=8) 89-103 96

Linearity

Samples were spiked with high concentrations of Mouse IP-10/CXCL10 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P17515
SynonymsC7, IFI10, INP10, SCYB10, crg-2, gIP-10, mob-1
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. RESEARCH IN VETERINARY SCIENCE (2019) IF: 1.751
    Amelioration effects of Kaempferol on immune response following chronic intermittent cold-stress

    DOI: 10.1016/j.rvsc.2019.08.012

    PMID: 31412308

    Sample: Serum,Tissue Homogenate
  2. ANTICANCER RESEARCH (2022) IF: 2.435
    Inhibition of STAT3 Promotes Effector T Cell Infiltration But also Immunosuppression in the HCC Tumor Microenvironment

    DOI: 10.21873/anticanres.16027

    Sample: Ril-175 Cell
  3. FEBS LETTERS (2022) IF: 4.124
    The Microglial membrane receptor TREM2 mediates exosome secretion to promote phagocytosis of amyloid-β by microglia

    DOI: 10.1002/1873-3468.14336

    PMID: 35292963

    Sample: Bv2 Cell
  4. INFLAMMATION RESEARCH (2021) IF: 4.575
    Inhibition of fibroblast growth factor-inducible 14 attenuates experimental tubulointerstitial fibrosis and profibrotic factor expression of proximal tubular epithelial cells

    DOI: 10.1007/s00011-021-01455-0

    PMID: 33755760

    Sample: Tissue Homogenate
  5. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
    The selective STING inhibitor H-151 preserves myocardial function and ameliorates cardiac fibrosis in murine myocardial infarction

    DOI: 10.1016/j.intimp.2022.108658

    PMID: 35278833

    Sample: Serum
  6. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 4.932
    COX-2-PGE2 signaling pathway contributes to hippocampal neuronal injury and cognitive impairment in PTZ-kindled epilepsy mice

    DOI: 10.1016/j.intimp.2020.106801

    Sample: Tissue Homogenate
  7. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2022) IF: 5.31
    Activation of the STING-IRF3 pathway involved in psoriasis with diabetes mellitus

    DOI: 10.1111/jcmm.17236

    Sample: Serum
  8. Frontiers in Immunology (2021) IF: 7.561
    Pathogenic Roles of CXCL10 in Experimental Autoimmune Prostatitis by Modulating Macrophage Chemotaxis and Cytokine Secretion

    DOI: 10.3389/fimmu.2021.706027

    PMID: 34659199

    Sample: Plasma,Prostate Tissue
  9. BIOLOGICAL PROCEDURES ONLINE (2022) IF: 7.717
    Angiotensin-converting enzyme 2 identifies immuno-hot tumors suggesting angiotensin-(1–7) as a sensitizer for chemotherapy and immunotherapy in breast cancer

    DOI: 10.1186/s12575-022-00177-9

    Sample: 4T1 Cell-Xenograft
  10. JOURNAL OF INVESTIGATIVE DERMATOLOGY (2021) IF: 8.551
    Successful Treatment of Vitiligo with Cold Atmospheric Plasma‒Activated Hydrogel

    DOI: 10.1016/j.jid.2021.04.019

    PMID: 34029575

    Sample: Serum,Tissue Homogenate
  11. Bioengineering & Translational Medicine (2022) IF: 10.711
    Baicalein self-microemulsion based on drug–phospholipid complex for the alleviation of cytokine storm

    DOI: 10.1002/btm2.10357

    Sample: Plasma
  12. CARBOHYDRATE POLYMERS (2022) IF: 10.723
    Aloe polymeric acemannan inhibits the cytokine storm in mouse pneumonia models by modulating macrophage metabolism

    DOI: 10.1016/j.carbpol.2022.120032

    PMID: 36184177

    Sample: Bronchoalveolar Fluid,Raw264.7 Cell
  13. Journal for ImmunoTherapy of Cancer (2022) IF: 12.469
    Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis

    DOI: 10.1136/jitc-2021-004381

    PMID: 35738798

    Sample: B16F10 Cell-Xenograft
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