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Mouse PⅠNP(Procollagen Ⅰ N-Terminal Propeptide) ELISA Kit

  • Cat.No.:E-EL-M0233

  • Reactivity: Mouse

To Purchase E-EL-M0233

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 93.75-6000 pg/mL
Sensitivity 56.25 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse PⅠNP in samples. No significant cross-reactivity or interference between Mouse PⅠNP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse PⅠNP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PⅠNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PⅠNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PⅠNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PⅠNP. You can calculate the concentration of Mouse PⅠNP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse PⅠNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse PⅠNP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 324.49 557.38 2793.45 353.21 570.31 2784.16
Standard deviation 20.57 23.91 126.54 24.16 32.91 126.68
CV (%) 6.34 4.29 4.53 6.84 5.77 4.55

Recovery

The recovery of Mouse PⅠNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-99 91
EDTA plasma (n=8) 93-105 98
Cell culture media (n=8) 95-106 100

Linearity

Samples were spiked with high concentrations of Mouse PⅠNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsP1NP, PINP, N-Propeptide Of Type I Procollagen, Procollagen I Amino Terminal Propeptide

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of the american chemical society (2020) IF: 14.612
    A smart nano-sacrificial layer on the bone surface prevents osteoporosis through acid-base neutralization regulated biocascade effects

    DOI: 10.1021/jacs.0c07309

    PMID: 32960592

    Sample: Serum
  2. Cell Death & Disease (2020) IF: 6.304
    PKC-δ deficiency in B cells displays osteopenia accompanied with upregulation of RANKL expression and osteoclast-osteoblast uncoupling

    DOI: 10.1038/s41419-020-02947-3

    PMID: 32938907

    Sample: Serum
  3. Cellular and Molecular Life Sciences (2020) IF: 6.496
    Maternal high protein-diet programs impairment of offspring's bone mass through miR-24-1-5p mediated targeting of SMAD5 in osteoblasts

    DOI: 10.1007/s00018-020-03608-6

    PMID: 32734584

    Sample: Serum
  4. Journal of Endocrinology (2019) IF: 4.041
    miR-487b-3p impairs osteoblastogenesis by targeting Notch-regulated ankyrin-repeat protein (Nrarp)

    DOI: 10.1530/JOE-19-0015

    PMID: 30978699

    Sample: Serum
  5. Journal of Molecular Medicine (2018) IF: 4.686
    Identification of novel microRNA inhibiting actin cytoskeletal rearrangement thereby suppressing osteoblast differentiation.

    DOI: 10.1007/s00109-018-1624-y

    PMID: 29523914

  6. Journal of molecular endocrinology (2018) IF: 3.562
    Ovariectomy-induced bone loss in TNFα and IL6 gene knockout mice is regulated by different mechanisms

    DOI: 10.1530/JME-17-0218

    PMID: 29339399

    Sample: Serum
  7. Endocrine (2015) IF: 3.131
    Preservation Of High-Fat Diet-Induced Femoral Trabecular Bone Loss Through Genetic Target Of Tnf-Α.

    DOI: 10.1007/s12020-015-0554-5

    PMID: 25700562

    Sample: Serum
  8. Nutrition Research and Practice (2018) IF: 1.635
    Effect of isoflavone-enriched whole soy milk powder supplementation on bone metabolism in ovariectomized mice.

    DOI: 10.4162/nrp.2018.12.4.275

    PMID: 30090164

    Sample: Serum
  9. AIP Conference Proceedings. AIP Publishing LLC (2019)
    The effect of Muntingia calabura L. leaves methanolic extract in increasing of collagen production

    DOI: 10.1063/1.5139830

    PMID: 99999999

    Sample: Cell culture supernatant
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