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Mouse PⅠNP(Procollagen Ⅰ N-Terminal Propeptide) ELISA Kit

  • Cat.No.:E-EL-M0233

  • Reactivity: Mouse

To Purchase E-EL-M0233

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 93.75-6000 pg/mL
Sensitivity 56.25 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse PⅠNP in samples. No significant cross-reactivity or interference between Mouse PⅠNP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse PⅠNP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PⅠNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PⅠNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PⅠNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PⅠNP. You can calculate the concentration of Mouse PⅠNP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse PⅠNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse PⅠNP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 324.49 557.38 2793.45 353.21 570.31 2784.16
Standard deviation 20.57 23.91 126.54 24.16 32.91 126.68
CV (%) 6.34 4.29 4.53 6.84 5.77 4.55

Recovery

The recovery of Mouse PⅠNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-99 91
EDTA plasma (n=8) 93-105 98
Cell culture media (n=8) 95-106 100

Linearity

Samples were spiked with high concentrations of Mouse PⅠNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsP1NP, N-Propeptide Of Type I Procollagen, Procollagen I Amino Terminal Propeptide
Research AreaSignal Transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Nature Communications (2022) IF: 14.919
    Mechanical force promotes dimethylarginine dimethylaminohydrolase 1-mediated hydrolysis of the metabolite asymmetric dimethylarginine to enhance bone formation

    DOI: 10.1038/s41467-021-27629-2

    PMID: 35013196

    Sample: Serum
  2. JOURNAL OF BONE AND MINERAL RESEARCH (2020) IF: 5.854
    Hymenialdisine: A Marine Natural Product That Acts on Both Osteoblasts and Osteoclasts and Prevents Estrogen-Dependent Bone Loss in Mice

    DOI: 10.1002/jbmr.4025

    Sample: Serum
  3. JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2022) IF: 6.117
    Maternal omega-3 LC-PUFA supplementation programs an improved bone mass in the offspring with a more pronounced effect in females than males at adulthood

    DOI: 10.1016/j.jnutbio.2022.109245

    Sample: Plasma
  4. International Journal of Biological Sciences (2022) IF: 6.582
    Myokine Irisin promotes osteogenesis by activating BMP/SMAD signaling via αV integrin and regulates bone mass in mice

    DOI: 10.7150/ijbs.63505

    PMID: 35002510

    Sample: Serum
  5. FASEB BioAdvances (2020)
    Low bone mineral density due to secondary hyperparathyroidism in the GlatmTg(CAG-A4GALT) mouse model of Fabry disease

    DOI: 10.1096/fba.2019-00080

    Sample: Plasma
  6. Nutrition Research and Practice (2018) IF: 1.635
    Effect of isoflavone-enriched whole soy milk powder supplementation on bone metabolism in ovariectomized mice

    DOI: 10.4162/nrp.2018.12.4.275

    Sample: Serum
  7. BIOELECTROMAGNETICS (2021) IF: 2.01
    Effects of Pulsed Electromagnetic Field Therapy at Different Frequencies on Bone Mass and Microarchitecture in Osteoporotic Mice

    DOI: 10.1002/bem.22344

    PMID: 34082467

    Sample: Serum
  8. JOURNAL OF MOLECULAR ENDOCRINOLOGY (2018) IF: 3.297
    Ovariectomy-induced bone loss in TNFα and IL6 gene knockout mice is regulated by different mechanisms

    DOI: 10.1530/JME-17-0218

    PMID: 29339399

    Sample: Serum
  9. ENDOCRINE (2015) IF: 3.878
    Preservation of high-fat diet-induced femoral trabecular bone loss through genetic target of TNF-α

    DOI: 10.1007/s12020-015-0554-5

    Sample: Serum
  10. CALCIFIED TISSUE INTERNATIONAL (2021) IF: 4.333
    Phenanthrenoid Coelogin Isolated from Coelogyne cristata Exerts Osteoprotective Effect Through MAPK-Mitogen-Activated Protein Kinase Signaling Pathway

    DOI: 10.1007/s00223-021-00818-3

    PMID: 33675370

    Sample: Serum
  11. JOURNAL OF ENDOCRINOLOGY (2019) IF: 4.381
    miR-487b-3p impairs osteoblastogenesis by targeting Notch-regulated ankyrin-repeat protein (Nrarp)

    DOI: 10.1530/JOE-19-0015

    PMID: 30978699

    Sample: Serum
  12. JOURNAL OF CELLULAR BIOCHEMISTRY (2022) IF: 4.48
    Maintenance of increased bone mass after PTH withdrawal by sequential medicarpin treatment via augmentation of cAMP-PKA pathway

    DOI: 10.1002/jcb.30313

    PMID: 35959633

    Sample: Serum
  13. JOURNAL OF CELLULAR PHYSIOLOGY (2019) IF: 4.522
    miR‐409‐5p negatively regulates Wnt/Beta catenin signaling pathway by targeting Lrp‐8

    DOI: 10.1002/jcp.28919

    Sample: Serum
  14. JOURNAL OF MOLECULAR MEDICINE-JMM (2018) IF: 4.938
    Identification of novel microRNA inhibiting actin cytoskeletal rearrangement thereby suppressing osteoblast differentiation

    DOI: 10.1007/s00109-018-1624-y

    Sample: Serum
  15. PHYTOMEDICINE (2022) IF: 5.34
    Activation of farnesoid X receptor signaling by geniposidic acid promotes osteogenesis

    DOI: 10.1016/j.phymed.2022.154258

    PMID: 35716540

    Sample: Serum
  16. Molecular Therapy-Nucleic Acids (2019) IF: 5.919
    MicroRNA-672-5p Identified during Weaning Reverses Osteopenia and Sarcopenia in Ovariectomized Mice

    DOI: 10.1016/j.omtn.2019.01.002

    Sample: Serum
  17. Cell Death & Disease (2020) IF: 6.304
    PKC-δ deficiency in B cells displays osteopenia accompanied with upregulation of RANKL expression and osteoclast–osteoblast uncoupling

    DOI: 10.1038/s41419-020-02947-3

    Sample: Serum
  18. JOURNAL OF CELLULAR PHYSIOLOGY (2021) IF: 6.384
    POLR2A blocks osteoclastic bone resorption and protects against osteoporosis by interacting with CREB1

    DOI: 10.1002/jcp.30220

    PMID: 33595106

    Sample: Serum
  19. CELLULAR AND MOLECULAR LIFE SCIENCES (2020) IF: 6.496
    Maternal high protein-diet programs impairment of offspring’s bone mass through miR-24-1-5p mediated targeting of SMAD5 in osteoblasts

    DOI: 10.1007/s00018-020-03608-6

    Sample: Serum
  20. Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
    Wheel-Running Exercise Protects Ovariectomized Mice from Bone Loss via IFN-γ-Mediated Suppression of the NF-κB and MAPK Pathways

    DOI: 10.1155/2022/2030818

    Sample: Serum
  21. Cancers (2022) IF: 6.575
    Novel Methods of Targeting IL-1 Signalling for the Treatment of Breast Cancer Bone Metastasis

    DOI: 10.3390/cancers14194816

    PMID: 36230739

    Sample: Serum
  22. Biomaterials Science (2021) IF: 6.843
    Irisin recouples osteogenesis and osteoclastogenesis to protect wear-particle-induced osteolysis by suppressing oxidative stress and RANKL production

    DOI: 10.1039/D1BM00563D

    PMID: 34323888

    Sample: Sera
  23. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
    Thiaplakortone B attenuates RANKL‐induced NF‐κB and MAPK signaling and dampens OVX‐induced bone loss in mice

    DOI: 10.1016/j.biopha.2022.113622

    Sample: Serum
  24. FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.101
    Chebulinic acid alleviates LPS-induced inflammatory bone loss by targeting the crosstalk between reactive oxygen species/NFκB signaling in osteoblast cells

    DOI: 10.1016/j.freeradbiomed.2022.11.026

    Sample: Serum
  25. FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.101
    A novel BMP2 secretagogue ameliorates glucocorticoid induced oxidative stress in osteoblasts by activating NRF2 dependent survival while promoting Wnt/β-catenin mediated osteogenesis

    DOI: 10.1016/j.freeradbiomed.2022.08.007

    PMID: 35963563

    Sample: Serum
  26. Cell Reports (2020) IF: 8.109
    SENP3 Suppresses Osteoclastogenesis by De-conjugating SUMO2/3 from IRF8 in Bone Marrow-Derived Monocytes

    DOI: 10.1016/j.celrep.2020.01.036

    Sample: Serum
  27. MOLECULAR THERAPY (2020) IF: 8.986
    CD39 Produced from Human GMSCs Regulates the Balance of Osteoclasts and Osteoblasts through the Wnt/β-Catenin Pathway in Osteoporosis

    DOI: 10.1016/j.ymthe.2020.04.003

    Sample: Serum,Plasma
  28. Redox Biology (2021) IF: 11.799
    CNC-bZIP protein NFE2L1 regulates osteoclast differentiation in antioxidant-dependent and independent manners

    DOI: 10.1016/j.redox.2021.102180

    PMID: 34763297

    Sample: Plasma
  29. EMBO Molecular Medicine (2022) IF: 14.26
    Sigma-1 receptor attenuates osteoclastogenesis by promoting ER-associated degradation of SERCA2

    DOI: 10.15252/emmm.202115373

    PMID: 35611810

    Sample: Serum
  30. Journal of the American Chemical Society (2020) IF: 14.612
    Smart Nanosacrificial Layer on the Bone Surface Prevents Osteoporosis through Acid–Base Neutralization Regulated Biocascade Effects

    DOI: 10.1021/jacs.0c07309

    PMID: 32960592

    Sample: Serum
  31. CELL DEATH AND DIFFERENTIATION (2021) IF: 15.828
    Site-1 protease controls osteoclastogenesis by mediating LC3 transcription

    DOI: 10.1038/s41418-020-00731-6

    PMID: 33469231

    Sample: Serum
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