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Mouse PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit

Citations(10)Uniprot : Q05769

Cat.No.:E-EL-M0959
Reactivity: Mouse

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse PTGS2/COX-2 in samples. No significant cross-reactivity or interference between Mouse PTGS2/COX-2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse PTGS2/COX-2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PTGS2/COX-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PTGS2/COX-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PTGS2/COX-2. You can calculate the concentration of Mouse PTGS2/COX-2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse PTGS2/COX-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse PTGS2/COX-2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.50	0.90	3.42	0.52	0.82	3.72
Standard deviation	0.03	0.05	0.15	0.03	0.04	0.18
CV (%)	6.00	5.56	4.39	5.77	4.88	4.84

Recovery

The recovery of Mouse PTGS2/COX-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-108	100
EDTA plasma (n=8)	90-101	96
Cell culture media (n=8)	95-108	102

Linearity

Samples were spiked with high concentrations of Mouse PTGS2/COX-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q05769
Synonyms	COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2
Research Area	Cancer, Cardiovascular, Metabolism, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

ACS Applied Materials & Interfaces (2022) IF: 10.383
Inflammation-Regulated Nanodrug Sensitizes Hepatocellular Carcinoma to Checkpoint Blockade Therapy by Reprogramming the Tumor Microenvironment

DOI: 10.1021/acsami.2c14448

PMID: 36314479
Sample: Hepa 1-6 cells-xenograft
Theranostics (2019) IF: 8.0630
Temporal inhibition of mouse mammary gland cancer metastasis by CORM-A1 and DETA/NO combination therapy

DOI: 10.7150/thno.31461

Sample: mammary gland tumor tissue
JOURNAL OF MEDICINAL CHEMISTRY (2020) IF: 6.205
Small-Molecule Antagonist Targeting Exportin-1 via Rational Structure-Based Discovery

DOI: 10.1021/acs.jmedchem.9b01663

Sample: serum
MOLECULAR IMMUNOLOGY (2022) IF: 4.407
The therapeutic potential of chondroitin sulfate in Aspergillus fumigatus keratitis

DOI: 10.1016/j.molimm.2022.04.007

PMID: 35504058
Sample: corneas,RAW 264.7 cell
Oxidative Medicine and Cellular Longevity (2019) IF: 4.868
Reactive Oxygen Species Are Involved in the Development of Gastric Cancer and Gastric Cancer-Related Depression through ABL1-Mediated Inflammation Signaling Pathway

DOI: 10.1155/2019/5813985

Sample: blood
INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
Daurisoline alleviated experimental colitis in vivo and in vitro: Involvement of NF-κB and Wnt/β-Catenin pathway

DOI: 10.1016/j.intimp.2022.108714

PMID: 35366641
Sample: colon tissues
BIOORGANIC & MEDICINAL CHEMISTRY (2020) IF: 3.073
Design and synthesis of 1,3-benzothiazinone derivatives as potential anti-inflammatory agents

DOI: 10.1016/j.bmc.2020.115526

Sample: Cell culture supernatant
CHEMICAL RESEARCH IN TOXICOLOGY (2022) IF: 3.739
P38 MAPK, NF-κB, and JAK-STAT3 Signaling Pathways Involved in Capecitabine-Induced Hand-Foot Syndrome via Interleukin 6 or Interleukin 8 Abnormal Expression

DOI: 10.1021/acs.chemrestox.1c00317

PMID: 35147423
Sample: Paw
Iranian Journal of Basic Medical Sciences (2021) IF: 2.699
Corilagin potential in inhibiting oxidative and inflammatory stress in LPS-induced murine macrophage cell lines (RAW 264.7)

DOI: 10.22038/IJBMS.2021.59348.13174

Sample: RAW 264.7 cell
Asian Pacific Journal of Tropical Biomedicine (2017) IF: 1.545
Anti-inflammatory properties of oolong tea (Camellia sinensis) ethanol extract and epigallocatechin gallate in LPS-induced RAW 264.7 cells

DOI: 10.1016/j.apjtb.2017.10.002

Sample: RAW 264.7 cell

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