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Myeloperoxidase (MPO) Peroxidation Activity Fluorometric Assay Kit

Cat:E-BC-F013 Citations (1)
Manual MSDS

Price: $ 240

Size:
96T
Quantity:
  • Sample Type: serum;plasma; animal tissue
  • Detection Instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
  • Reasearch Areas: Oxidative stress
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For research use only. Order now, ship in 3 days

Product Details
Detection Principle Under the catalysis of peroxidase, hydrogen peroxide can oxidize the non-fluorescent probe into the fluorescent substance, and its fluorescence intensity is proportional to the total peroxidase activity in the sample. This kit specifically inhibits the peroxidase activity of MPO in the sample through an MPO enzyme inhibitor, thus distinguishing the peroxidase activity of MPO in the sample from that of other peroxidases.
Synonyms MPO
Sample Type serum,plasma, animal tissue
Sensitivity 0.001 U/L
Detection Range 0.001 - 1.26 U/L
Detection Method Fluorescence method
Assay Time 20 min
Precision inter-assay CV: 5.4% | intra-assay CV: 1%
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Porcine serum 5-10
Rabbit serum 3-5
Rat serum 2-5
Mouse serum 10-20
Mouse plasma 30-50
Horse serum 2-5
10% Rat heart tissue homogenate 1
10% Rat lung tissue homogenate 1
The diluent is buffer solution. For the dilution of other sample types, please do pretest to confirm the dilution factor.