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NA/NE(Noradrenaline/Norepinephrine) ELISA Kit

  • Cat.No.:E-EL-0047

  • Reactivity: Universal

To Purchase E-EL-0047

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes NA/NE in samples. No significant cross-reactivity or interference between NA/NE and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of NA/NE concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with NA/NE. During the reaction, NA/NE in the sample or standard competes with a fixed amount of NA/NE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to NA/NE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NA/NE in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level NA/NE were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level NA/NE were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.10 2.30 9.50 1.00 2.40 9.60
Standard deviation 0.10 0.10 0.30 0.10 0.10 0.40
CV (%) 9.09 4.35 3.16 10.00 4.17 4.17

Recovery

The recovery of NA/NE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-99 93
EDTA plasma(n=8) 94-110 102
Cell culture media(n=8) 89-102 97

Linearity

Samples were spiked with high concentrations of NA/NE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsNorepinephrine, NE, noradrenaline, NA, NAd, norad, 4, 5-β-trihydroxyphenethylamine

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of Experimental & Clinical Cancer Research (2020) IF: 7.068
    Norepinephrine-stimulated HSCs secrete sFRP1 to promote HCC progression following chronic stress via augmentation of a Wnt16B/β-catenin positive feedback loop

    DOI: 10.1186/s13046-020-01568-0

    PMID: 99999999

    Sample: Serum,Cell culture supernatant
  2. Cellular Physiology and Biochemistry (2018) IF: 5.104
    Renal Denervation Effects on Myocardial Fibrosis and Ventricular Arrhythmias in Rats with Ischemic Cardiomyopathy.

    DOI: 10.1159/000489653

    PMID: 29742489

  3. Cellular Physiology and Biochemistry (2016) IF: 5.104
    Renal Denervation Attenuates Multi-Organ Fibrosis And Improves Vascular Remodeling In Rats With Transverse Aortic Constriction Induced Cardiomyopathy..

    DOI: 10.1159/000452561

    PMID: 27889753

    Sample: Plasma,Tissue homogenate
  4. Journal of the American Heart Association (2017) IF: 4.644
    Atrial Fibrillation In Acute Obstructive Sleep Apnea: Autonomic Nervous Mechanism And Modulation.

    DOI: 10.1161/JAHA.117.006264

    PMID: 28903938

    Sample: Plasma
  5. Scientific Reports (2016) IF: 4.259
    FGF21 Ameliorates The Neurocontrol Of Blood Pressure In The High Fructose-Drinking Rats.

    DOI: 10.1038/srep29582

    PMID: 27387420

    Sample: Serum
  6. Journal of cellular physiology (2019) IF: 3.923
    Norepinephrine stimulation downregulates the β2-恆drenergic receptor-搉itric oxide pathway in human pulmonary artery endothelial cells

    DOI: 10.1002/jcp.27057

    PMID: 30132872

    Sample: Plasma
  7. Mediators Of Inflammation (2015) IF: 3.232
    Intranasal Dexmedetomidine On Stress Hormones, Inflammatory Markers, And Postoperative Analgesia After Functional Endoscopic Sinus Surgery.

    DOI: 10.1155/2015/939431

    PMID: 26199465

    Sample: Serum
  8. Frontiers in Neural Circuits (2017) IF: 3.005
    The Locus Coeruleus-Norepinephrine System Mediates Empathy For Pain Through Selective Up-Regulation Of P2X3 Receptor In Dorsal Root Ganglia In Rats.

    DOI: 10.3389/fncir.2017.00066

    PMID: 28979194

    Sample: Serum
  9. American Journal of Hypertension (2016) IF: 3.541
    A Novel Swine Model Of Spontaneous Hypertension With Sympathetic Hyperactivity Responds Well To Renal Denervation.

    DOI: 10.1093/ajh/hpv066

    PMID: 25958303

    Sample: Plasma,Urine
  10. Molecular Medicine Reports (2020) IF: 2.1
    Effects of botulinum toxin A on endometriosis-associated pain and its related mechanism

    DOI: 10.3892/mmr.2020.11501

    PMID: 33000241

    Sample: Cell culture supernatant,Tissue homogenate
  11. PloS one (2018) IF: 2.766
    Selective ablation of the ligament of Marshall reduces ischemia and reperfusion-induced ventricular arrhythmias

    DOI: 10.1371/journal.pone.0203083

    PMID: 30153281

    Sample: Serum
  12. Neuroscience Letters (2019) IF: 2.159
    Differentiation of human glioblastoma U87 cells into cholinergic neuron

    DOI: 10.1016/j.neulet.2019.03.049

    PMID: 30928478

    Sample: Cell culture supernatant
  13. Environmental toxicology (2019) IF: 2.491
    Oxidative stress and neuromodulatory effects of deltamethrin and its combination with insect repellents in rats

    DOI: 10.1002/tox.22741

    PMID: 30884102

    Sample: Serum
  14. Journal of cardiovascular electrophysiology (2018) IF: 2.873
    Selective ablation of the ligament of Marshall attenuates atrial electrical remodeling in a short‐term rapid atrial pacing canine model.

    DOI: 10.1111/jce.13658

    PMID: 29864240

    Sample: Serum
  15. Sleep and Breathing (2017) IF: 2.288
    The Role Of The Nox4-Derived ROS-Mediated RhoA/Rho Kinase Pathway In Rat Hypertension Induced By Chronic Intermittent Hypoxia.

    DOI: 10.1007/s11325-016-1449-2

    PMID: 28078487

    Sample: Serum,Tissue homogenate
  16. Turkish Journal of Science (2019)
    The Effect of Peripheral Adropin Application on Hypothalamic Pituitary Adrenal Axis in Rats

    PMID: 99999999

    Sample: Tissue homogenate
  17. IOP Conference Series: Materials Science and Engineering. IOP Publishing (2018)
    Research on the evaluation indexes of animal model of liver-fire hypertension syndrome

    DOI: 10.1088/1757-899X/397/1/012132

    PMID: 99999999

    Sample: Serum
  18. Journal of Pharmaceutical Sciences and Research (2019)
    Effect of chronic toxoplasmosis on levels of some neurotransmitters (Dopamine, Adrenaline, and Noradrenaline) in human Serum

    PMID: 99999999

    Sample: Serum
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