Click here to view cell apoptosis assay products

NA/NE(Noradrenaline/Norepinephrine) ELISA Kit

  • Cat.No.:E-EL-0047

  • Reactivity: Universal

To Purchase E-EL-0047

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes NA/NE in samples. No significant cross-reactivity or interference between NA/NE and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of NA/NE concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with NA/NE. During the reaction, NA/NE in the sample or standard competes with a fixed amount of NA/NE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to NA/NE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NA/NE in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level NA/NE were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level NA/NE were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.10 2.30 9.50 1.00 2.40 9.60
Standard deviation 0.10 0.10 0.30 0.10 0.10 0.40
CV (%) 9.09 4.35 3.16 10.00 4.17 4.17


The recovery of NA/NE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-99 93
EDTA plasma(n=8) 94-110 102
Cell culture media(n=8) 89-102 97


Samples were spiked with high concentrations of NA/NE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsNorepinephrine, NE, noradrenaline, NA, NAd, norad, 4, 5-β-trihydroxyphenethylamine
Research AreaNeuroscience, Cardiovascular

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Journal of Experimental & Clinical Cancer Research (2020) IF: 7.068
    Norepinephrine-stimulated HSCs secrete sFRP1 to promote HCC progression following chronic stress via augmentation of a Wnt16B/β-catenin positive feedback loop

    DOI: 10.1186/s13046-020-01568-0

    Sample: Serum,Cell culture supernatant
  2. Cellular Physiology and Biochemistry (2018) IF: 5.104
    Renal Denervation Effects on Myocardial Fibrosis and Ventricular Arrhythmias in Rats with Ischemic Cardiomyopathy.

    DOI: 10.1159/000489653

    PMID: 29742489

  3. Cellular Physiology and Biochemistry (2016) IF: 5.104
    Renal Denervation Attenuates Multi-Organ Fibrosis And Improves Vascular Remodeling In Rats With Transverse Aortic Constriction Induced Cardiomyopathy..

    DOI: 10.1159/000452561

    PMID: 27889753

    Sample: Plasma,Tissue homogenate
  4. Journal of the American Heart Association (2017) IF: 4.644
    Atrial Fibrillation In Acute Obstructive Sleep Apnea: Autonomic Nervous Mechanism And Modulation.

    DOI: 10.1161/JAHA.117.006264

    PMID: 28903938

    Sample: Plasma
  5. Scientific Reports (2016) IF: 4.259
    FGF21 Ameliorates The Neurocontrol Of Blood Pressure In The High Fructose-Drinking Rats.

    DOI: 10.1038/srep29582

    PMID: 27387420

    Sample: Serum
  6. Journal of cellular physiology (2019) IF: 3.923
    Norepinephrine stimulation downregulates the β2-恆drenergic receptor-搉itric oxide pathway in human pulmonary artery endothelial cells

    DOI: 10.1002/jcp.27057

    PMID: 30132872

    Sample: Plasma
  7. Mediators Of Inflammation (2015) IF: 3.232
    Intranasal Dexmedetomidine On Stress Hormones, Inflammatory Markers, And Postoperative Analgesia After Functional Endoscopic Sinus Surgery.

    DOI: 10.1155/2015/939431

    PMID: 26199465

    Sample: Serum
  8. Frontiers in Neural Circuits (2017) IF: 3.005
    The Locus Coeruleus-Norepinephrine System Mediates Empathy For Pain Through Selective Up-Regulation Of P2X3 Receptor In Dorsal Root Ganglia In Rats.

    DOI: 10.3389/fncir.2017.00066

    PMID: 28979194

    Sample: Serum
  9. American Journal of Hypertension (2016) IF: 3.541
    A Novel Swine Model Of Spontaneous Hypertension With Sympathetic Hyperactivity Responds Well To Renal Denervation.

    DOI: 10.1093/ajh/hpv066

    PMID: 25958303

    Sample: Plasma,Urine
  10. Molecular Medicine Reports (2020) IF: 2.1
    Effects of botulinum toxin A on endometriosis-associated pain and its related mechanism

    DOI: 10.3892/mmr.2020.11501

    PMID: 33000241

    Sample: Cell culture supernatant,Tissue homogenate
  11. PloS one (2018) IF: 2.766
    Selective ablation of the ligament of Marshall reduces ischemia and reperfusion-induced ventricular arrhythmias

    DOI: 10.1371/journal.pone.0203083

    PMID: 30153281

    Sample: Serum
  12. Neuroscience Letters (2019) IF: 2.159
    Differentiation of human glioblastoma U87 cells into cholinergic neuron

    DOI: 10.1016/j.neulet.2019.03.049

    PMID: 30928478

    Sample: Cell culture supernatant
  13. Environmental toxicology (2019) IF: 2.491
    Oxidative stress and neuromodulatory effects of deltamethrin and its combination with insect repellents in rats

    DOI: 10.1002/tox.22741

    PMID: 30884102

    Sample: Serum
  14. Journal of cardiovascular electrophysiology (2018) IF: 2.873
    Selective ablation of the ligament of Marshall attenuates atrial electrical remodeling in a short‐term rapid atrial pacing canine model.

    DOI: 10.1111/jce.13658

    PMID: 29864240

    Sample: Serum
  15. Sleep and Breathing (2017) IF: 2.288
    The Role Of The Nox4-Derived ROS-Mediated RhoA/Rho Kinase Pathway In Rat Hypertension Induced By Chronic Intermittent Hypoxia.

    DOI: 10.1007/s11325-016-1449-2

    PMID: 28078487

    Sample: Serum,Tissue homogenate
  16. Turkish Journal of Science (2019)
    The Effect of Peripheral Adropin Application on Hypothalamic Pituitary Adrenal Axis in Rats
    Sample: Tissue homogenate
  17. IOP Conference Series: Materials Science and Engineering. IOP Publishing (2018)
    Research on the evaluation indexes of animal model of liver-fire hypertension syndrome

    DOI: 10.1088/1757-899X/397/1/012132

    Sample: Serum
  18. Journal of Pharmaceutical Sciences and Research (2019)
    Effect of chronic toxoplasmosis on levels of some neurotransmitters (Dopamine, Adrenaline, and Noradrenaline) in human Serum
    Sample: Serum
Show AllShorten


  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for Free Shipping 24T ELISA Kit
*Product Name:
*Catalog Number:
*When will you use it?