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NA/NE(Noradrenaline/Norepinephrine) ELISA Kit (E-EL-0047)

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Citations ({{ citationsPage.numTotal }}) Manual MSDS
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All Size Price Qty
96T $ 495.00
48T $ 396.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 0.19 ng/mL
Detection Range 0.31-20 ng/mL
Sample Volume 50 μL
Total Assay Time 2 h 30 min
Reacitivity Universal
Specificity This kit recognizes Universal NA/NE in samples.No significant cross-reactivity or interference between Universal NA/NE and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Competitive
Assay Type Competitive-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal NA/NE. During the reaction, Universal NA/NE in the sample or standard competes with a fixed amount of Universal NA/NE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal NA/NE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal NA/NE in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Norepinephrine (INN name: Norepinephrine, also known as Noradrenaline, abbreviated NE or NA), also known as norepinephrine, is a substance formed after the removal of N-methyl group of adrenaline, and also belongs to catecholamines in chemical structure. It is both a neurotransmitter, mainly synthesized and secreted by sympathetic postganglionic neurons and adrenergic nerve endings in the brain, which is the main transmitter released by the latter, and a hormone, synthesized and secreted by the adrenal medulla, but in smaller amounts.
Research Area Neuroscience , Cardiovascular
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