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NCAM1 / CD56 Polyclonal Antibody

Cat:E-AB-62136
Manual MSDS
  • +1

Price: $ 530

Price: $ 320

Price: $ 200

Size:
200μL 120μL 60μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC;IF
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For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:Mouse brain,Mouse spinal cord,Rat brain
Verified Samples in IHC:Human stomach
Verified Samples in IF:C6
Dilution

WB 1:500-1:2000, IHC 1:100-1:200, IF 1:50-1:200

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant fusion protein of human NCAM1 / CD56 (NP_851996.2).
Abbre NCAM1 / CD56
Synonyms CD56;MSK39;NCAM;NCAM1;NCAM1 /CD56
Swissprot
Calculated MW 40kDa/73kDa/80kDa/83kDa/93kDa/94kDa
Observed MW 160kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Secreted and Cell membrane.
Concentration 1mg/mL
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method Affinity purification
Research Areas Cancer; Neuroscience; Stem cells
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants.