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NDRG4 Polyclonal Antibody

Cat:E-AB-53285
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human; Mouse; Rat
  • Applications: WB;IHC
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Product Details
Verified Samples Verified Samples in WB:RAW264.7,Rat brain,Mouse brain
Verified Samples in IHC:Human liver cancer
Dilution

WB 1:500-1:2000, IHC 1:50-1:100

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthetic peptide of human NDRG4
Abbre NDRG4
Synonyms BDM1;Brain development-related molecule 1;KIAA1180;Ndrg4;NDRG4;Protein NDRG4;SMAP 8;SMAP-8;Vascular smooth muscle cell-associated protein 8
Swissprot
Calculated MW 38 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytosol,Endoplasmic reticulum,endoplasmic reticulum membrane,Mitochondrion,Plasma Membrane,basolateral plasma membrane,cell projection membrane,Other locations:cytoplasm
Concentration 1.26 mg/mL
Buffer PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Purification Method Antigen affinity purification
Research Areas Neuroscience; Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that is required for cell cycle progression and survival in primary astrocytes and may be involved in the regulation of mitogenic signalling in vascular smooth muscles cells. Alternative splicing results in multiple transcripts encoding different isoforms.