NEK2 Polyclonal Antibody
Price: $ 399
Price: $ 240
Price: $ 143
Price: $ 73
Size:
200μL
120μL
60μL
20μL
Quantity:
- Host: Rabbit
- Reactivity: Human;Mouse
- Applications: WB
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Product Details
Verified Samples |
Verified Samples in WB:LoVo |
Dilution |
WB 1:500-1:2000 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Recombinant protein of human NEK2 |
Abbre | NEK2 |
Synonyms | HSPK 21;HsPK21;NEK 2;NEK2;NEK2;NEK2A;Never in mitosis A-related kinase 2;NIMA (never in mitosis gene a) related kinase 2;NimA like protein kinase 1;NIMA related kinase 2;NimA related protein kinase 2;NimA-like protein kinase 1;NimA-related protein kinase 2;NLK 1;NLK1;Serine/threonine-protein kinase Nek2 |
Swissprot | |
Calculated MW | 52kDa |
Cellular Localization | Cytoplasm. Predominantly cytoplasmic; Nucleus>nucleolus. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end and Nucleus. Chromosome>centromere. Chromosome>centromere>kinetochore. Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. |
Concentration | 0.3 mg/mL |
Buffer | PBS with 0.05% sodium azide and 50% glycerol, PH7.4 |
Purification Method | Affinity purification |
Research Areas | Cancer; Cell Biology; Signal Transduction |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Biological ice pack at 4 ℃ |
background | This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. |
Experiment Operation Video
Protocols
• Western Blot Guide
• Immunohistochemistry Guide for Slide-mounted Paraffin Sections
• Immunofluorescence Guide
• Immunohistochemistry Troubleshooting Tips
• Western Blot Troubleshooting Tips
• Immunofluorescence Troubleshooting Tips
• Comparison of Protein Separation Linear Range
• ImmunoPrecipitation Protocol