Nitric Oxide (NO) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K035-M

      Size:
      • 96T
      • 48T
      Qty:
      - +
      Price: $180

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(540-550 nm)

      Valid period: 6 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      NO is easily oxidized to form NO2- in vivo or in aqueous solution, and a reddish azo compound is formed with the color developing agent, and the concentration of the azo compound is linearly related to the concentration of NO. The concentration of NO can be calculated indirectly by measuring the OD value at 550 nm.

      Performance characteristics

      Synonyms NO
      Sample type serum,plasma,cells,cell culture supernatant,tissue
      Sensitivity 0.16 μmol/L
      Detection range 0.16-300 μmol/L
      Precision Average inter-assay CV: 3.7%Average intra-assay CV: 2.4%
      Other instruments required Micropipettor, Vortex mixer, Centrifuge
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months

      Dilution of sample


      Citations

      1. Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice.
        IF: 2.518
        Journal: Biomedical and Environmental Sciences(2018)
        DOI: 10.3967/bes2018.101
        PMID: 30423277
        Products: E-EL-M0049 E-EL-M0044 E-EL-M0077 E-EL-M0687 E-EL-M0048 E-EL-M2730 E-EL-M0400 E-EL-M0914 E-EL-0060 E-EL-M0419 E-EL-M0618 E-BC-K035-M E-BC-K020-M 
        Sample type: Plasma,Tissue homogenate
        Reactivity: Mouse
      2. GPR30 Attenuates Myocardial Fibrosis in Diabetic Ovariectomized Female Rats: Role of iNOS Signaling
        IF: 2.918
        Journal: DNA and Cell Biology(2018)
        DOI: 10.1089/dna.2018.4208
        PMID: 30227089
        Products: E-BC-K035-M 
        Sample type: Serum
        Reactivity: Rat
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