Nitric Oxide (NO) Colorimetric Assay Kit
Price: $ 150
Price: $ 120
- Sample Type: serum;plasma;tissue;saliva
- Detection Instrument: Microplate reader(540-550 nm)
- Reasearch Areas: Oxidative stress
For research use only. Order now, ship in 3 days
Detection Principle | NO is easily oxidized to form NO2- in vivo or in aqueous solution, and a reddish azo compound is formed with the color developing agent, and the concentration of the azo compound is linearly related to the concentration of NO. The concentration of NO can be calculated indirectly by measuring the OD value at 550 nm. | ||||||||||||||
Synonyms | NO | ||||||||||||||
Sample Type | serum,plasma,tissue,saliva | ||||||||||||||
Sensitivity | 0.16 μmol/L | ||||||||||||||
Detection Range | 0.16-100 μmol/L | ||||||||||||||
Detection Method | Colorimetric method | ||||||||||||||
Assay Time | 55 min | ||||||||||||||
Precision | inter-assay CV: 3.7% | intra-assay CV: 2.4% | ||||||||||||||
Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) | ||||||||||||||
Storage | This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. | ||||||||||||||
Valid Period | 12 months | ||||||||||||||
Images | |||||||||||||||
Dilution Of Sample |
The recommended dilution factor for different samples is as follows (for reference only):
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The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor. |
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