Nitric Oxide (NO) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K035-M

      • 96T
      • 48T
      - +
      Price: $150
      Can detect 80 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(540-550 nm)

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Detection principle

      NO is easily oxidized to form NO2- in vivo or in aqueous solution, and a reddish azo compound is formed with the color developing agent, and the concentration of the azo compound is linearly related to the concentration of NO. The concentration of NO can be calculated indirectly by measuring the OD value at 550 nm.

      Performance characteristics

      Synonyms NO
      Sample type Serum, plasma, tissue, saliva
      Sensitivity 0.16 μmol/L
      Detection range 0.16-100 μmol/L
      Detection method Colorimetric method
      Assay type Quantitative
      Assay time 55 min
      Precision Average inter-assay CV: 3.7%Average intra-assay CV: 2.4%
      Other instruments required Micropipettor, Vortex mixer, Centrifuge
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months

      Dilution of sample


      1. GPR30 Attenuates Myocardial Fibrosis in Diabetic Ovariectomized Female Rats: Role of iNOS Signaling
        IF: 2.918
        Journal: DNA and Cell Biology(2018)
        DOI: 10.1089/dna.2018.4208
        PMID: 30227089
        Products: E-BC-K035-M 
        Sample type: Serum
        Reactivity: Rat
      2. Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice.
        IF: 2.518
        Journal: Biomedical and Environmental Sciences(2018)
        DOI: 10.3967/bes2018.101
        PMID: 30423277
        Products: E-BC-K035-M E-BC-K020-M 
        Sample type: Plasma,Tissue homogenate
        Reactivity: Mouse
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      • Q&A (1)

      K*****nSubmitted [ Dec 14 2020 ]

      Asked: Can the kit use for HUVEC Cell? How much does it ship to Thailand. Thanks.



      adminSubmitted [ Dec 14 2020 ]

      Answered: Hi Korawan, Sorry to tel you this kit can't test cell samples. Any questions, pls feel free to ask. Best regards,

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      MSDS for ELISA ISO 9001

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