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Nitric Oxide (NO) Colorimetric Assay Kit

Cat:E-BC-K035-M Citations (46)
Manual MSDS

Price: $ 150

Price: $ 120

Size:
96T 48T
Quantity:
  • Sample Type: serum;plasma;tissue;saliva
  • Detection Instrument: Microplate reader(540-550 nm)
  • Reasearch Areas: Oxidative stress
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Product Details
Detection Principle NO is easily oxidized to form NO2- in vivo or in aqueous solution, and a reddish azo compound is formed with the color developing agent, and the concentration of the azo compound is linearly related to the concentration of NO. The concentration of NO can be calculated indirectly by measuring the OD value at 550 nm.
Synonyms NO
Sample Type serum,plasma,tissue,saliva
Sensitivity 0.16 μmol/L
Detection Range 0.16-100 μmol/L
Detection Method Colorimetric method
Assay Time 55 min
Precision inter-assay CV: 3.7% | intra-assay CV: 2.4%
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Valid Period 12 months
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Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 1
Human plasma 1
Rat serum 1
Rat plasma 1
10% Mouse liver tissue homogenization 1
10% Epipremnum aureum tissue homogenization 1
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.